New tools for high-throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastoris

https://portalciencia.ull.es/documentos/5e3c3f5929995246bbf616bf

Detalhes bibliográficos
Autores: González Carracedo, Mario Andrés, Brito, Nélida, Hernández Bolaños, Eduardo, González, Celedonio
Formato: artículo
Fecha de publicación:2019
País:España
Recursos:Universidad de La Laguna (ULL)
Repositorio:RIULL. Repositorio Institucional de la Universidad de La Laguna
OAI Identifier:oai:riull.ull.es:915/41764
Acesso em linha:http://riull.ull.es/xmlui/handle/915/41764
Access Level:acceso abierto
Palavra-chave:fungal secretory proteins
Saccharomyces cerevisiae
Pichia pastoris
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spelling New tools for high-throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastorisGonzález Carracedo, Mario AndrésBrito, NélidaHernández Bolaños, EduardoGonzález, Celedoniofungal secretory proteinsSaccharomyces cerevisiaePichia pastorishttps://portalciencia.ull.es/documentos/5e3c3f5929995246bbf616bfHeterologous protein expression in yeast, mostly in Saccharomyces cerevisiae and Pichia pastoris, is a well-established and widely used technique. It typically requires the construction of an expression vector in Escherichia coli containing the foreign gene and its subsequent transformation into yeast. Although simple, this procedure has important limitations for the expression of large numbers of proteins, that is, for the generation of protein libraries. We describe here the development of a novel system for the easy and fast expression of heterologous proteins both in S. cerevisiae and in P. pastoris, under the control of the GAL1 and AOX1 promoters respectively. Expression in S. cerevisiae requires only the transformation of yeast cells with an unpurified PCR product carrying the gene to be expressed, and the expression of the same gene in P. pastoris requires only the isolation of the plasmid generated in S. cerevisiae and its transformation into this second yeast, thus making this system suitable for high-throughput projects. The system has been tested by the extracellular expression of 30 secretory fungal proteins.Bioquímica, Microbiología, Biología Celular y Genética202520252019info:eu-repo/semantics/articleapplication/pdfhttp://riull.ull.es/xmlui/handle/915/41764reponame:RIULL. Repositorio Institucional de la Universidad de La Lagunainstname:Universidad de La Laguna (ULL)InglésMicrobial Biotechnology (2019) 12(6), 1139–1153Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ESoai:riull.ull.es:915/417642026-06-22T13:13:57Z
dc.title.none.fl_str_mv New tools for high-throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastoris
title New tools for high-throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastoris
spellingShingle New tools for high-throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastoris
González Carracedo, Mario Andrés
fungal secretory proteins
Saccharomyces cerevisiae
Pichia pastoris
title_short New tools for high-throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastoris
title_full New tools for high-throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastoris
title_fullStr New tools for high-throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastoris
title_full_unstemmed New tools for high-throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastoris
title_sort New tools for high-throughput expression of fungal secretory proteins in Saccharomyces cerevisiae and Pichia pastoris
dc.creator.none.fl_str_mv González Carracedo, Mario Andrés
Brito, Nélida
Hernández Bolaños, Eduardo
González, Celedonio
author González Carracedo, Mario Andrés
author_facet González Carracedo, Mario Andrés
Brito, Nélida
Hernández Bolaños, Eduardo
González, Celedonio
author_role author
author2 Brito, Nélida
Hernández Bolaños, Eduardo
González, Celedonio
author2_role author
author
author
dc.contributor.none.fl_str_mv Bioquímica, Microbiología, Biología Celular y Genética
dc.subject.none.fl_str_mv fungal secretory proteins
Saccharomyces cerevisiae
Pichia pastoris
topic fungal secretory proteins
Saccharomyces cerevisiae
Pichia pastoris
description https://portalciencia.ull.es/documentos/5e3c3f5929995246bbf616bf
publishDate 2019
dc.date.none.fl_str_mv 2019
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://riull.ull.es/xmlui/handle/915/41764
url http://riull.ull.es/xmlui/handle/915/41764
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Microbial Biotechnology (2019) 12(6), 1139–1153
dc.rights.none.fl_str_mv Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ES
rights_invalid_str_mv Licencia Creative Commons (Reconocimiento-No comercial-Sin obras derivadas 4.0 Internacional)
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es_ES
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:RIULL. Repositorio Institucional de la Universidad de La Laguna
instname:Universidad de La Laguna (ULL)
instname_str Universidad de La Laguna (ULL)
reponame_str RIULL. Repositorio Institucional de la Universidad de La Laguna
collection RIULL. Repositorio Institucional de la Universidad de La Laguna
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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