| Sumario: | Environmental/experimental conditions: Arabidopsis (A. thaliana) WT, ecotype Columbia-0, T-DNA insertion mutants, and transgenic lines (Supplementary Table S4) were routinely grown on soil in growth chambers under long-day (LD) (16 h light/8 h darkness) or SD (8 h light/16 h darkness) photoperiod at 22 and 20 °C during light and dark periods, respectively, and a light intensity of 125 µE m−2 s−1 unless otherwise stated. For HL conditions, 6-wk-old SD grown plants were transferred to continuous HL (930 µE m−2 s−1) for 7 d. T-DNA insertion mutants 2cpab (Ojeda et al. 2018), Δ2cp (Pulido et al. 2010), ntrc (Serrato et al. 2004), prxQ (Lamkemeyer et al. 2006), prxIIE (Romero-Puertas et al. 2007), and fad7 and fad8 (Román et al. 2015) were previously reported. Transgenic lines overexpressing 2CPA (2CPA-OE), NTRC (NTRC-OE) or FAD8 (WT/FAD8–YFP–HA) under the control of the CaMV 35S promoter in the Col-0 background, were previously described by Pérez-Ruiz et al. (2017), Ojeda et al. (2018), and Román et al. (2015), respectively. Transgenic lines 2cpab/2CPA-OE, 2cpab/2CPA-CP-S-OE, 2cpab/FAD8–YFP–HA, WT/FAD8–CFP–HA, and 2cpab/FAD8–CFP–HA were generated in this study as described below. Escherichia coli and Agrobacterium tumefaciens (strain GV3101) were grown in liquid Miller nutrient at 37 and 28 °C, respectively, with the appropriate antibiotics.
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