Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related Genes

[EN] Several Trichoderma spp. are well known for their ability to: (i) act as important biocontrol agents against phytopathogenic fungi; (ii) function as biofertilizers; (iii) increase the tolerance of plants to biotic and abiotic stresses; and (iv) induce plant defense responses via the production...

Full description

Bibliographic Details
Authors: Gomes, Eriston V., Ulhoa, Cirano J., Cardoza, Rosa E., Silva, Roberto N., Gutiérrez , Santiago 1965-
Format: article
Status:Published version
Publication Date:2017
Country:España
Institution:Universidad de León
Repository:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/19105
Online Access:https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.00880/full
https://hdl.handle.net/10612/19105
Access Level:Open access
Keyword:Biología
Bioquímica
Trichoderma harzianum
Epl-1 protein
Botrytis cinerea
Fungus–plant interaction
Mycoparasitism
id ES_1da8b44f0b92087bc2e7d29bbf1c87f0
oai_identifier_str oai:buleria.unileon.es:10612/19105
network_acronym_str ES
network_name_str España
repository_id_str
spelling Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related GenesGomes, Eriston V.Ulhoa, Cirano J.Cardoza, Rosa E.Silva, Roberto N.Gutiérrez , Santiago 1965-BiologíaBioquímicaTrichoderma harzianumEpl-1 proteinBotrytis cinereaFungus–plant interactionMycoparasitism[EN] Several Trichoderma spp. are well known for their ability to: (i) act as important biocontrol agents against phytopathogenic fungi; (ii) function as biofertilizers; (iii) increase the tolerance of plants to biotic and abiotic stresses; and (iv) induce plant defense responses via the production and secretion of elicitor molecules. In this study, we analyzed the gene-regulation effects of Trichoderma harzianum Epl-1 protein during the interactions of mutant ∆epl-1 or wild-type T. harzianum strains with: (a) the phytopathogen Botrytis cinerea and (b) with tomato plants, on short (24 h hydroponic cultures) and long periods (4-weeks old plants) after Trichoderma inoculation. Our results indicate that T. harzianum Epl-1 protein affects the in vitro expression of B. cinerea virulence genes, especially those involved in the botrydial biosynthesis (BcBOT genes), during the mycoparasitism interaction. The tomato defense-related genes were also affected, indicating that Epl-1 is involved in the elicitation of the salicylic acid pathway. Moreover, Epl-1 also regulates the priming effect in host tomato plants and contributes to enhance the interaction with the host tomato plant during the early stage of root colonizationSIThis work was supported by the State of São Paulo Research Foundation (FAPESP) (Proc. 2012/16895-4, Proc. 2013/24452-8, and Proc. 2016/04274-6). Funding was also obtained from the Junta de Castilla y León, Spain (LE228U14) and the Spanish Government Grants from Ministerio de Economía y Competitividad (AGL2012-40041-C02-02 and AGL2015-70671-C2-2-R)Frontiers MediaMicrobiologiaEscuela de Ingeniería Agraria y Forestal2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.00880/fullhttps://hdl.handle.net/10612/19105reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónIngléshttp://creativecommons.org/licenses/by-nd/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/191052026-06-24T12:43:27Z
dc.title.none.fl_str_mv Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related Genes
title Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related Genes
spellingShingle Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related Genes
Gomes, Eriston V.
Biología
Bioquímica
Trichoderma harzianum
Epl-1 protein
Botrytis cinerea
Fungus–plant interaction
Mycoparasitism
title_short Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related Genes
title_full Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related Genes
title_fullStr Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related Genes
title_full_unstemmed Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related Genes
title_sort Involvement of Trichoderma harzianum Epl-1 Protein in the Regulation of Botrytis Virulence- and Tomato Defense-Related Genes
dc.creator.none.fl_str_mv Gomes, Eriston V.
Ulhoa, Cirano J.
Cardoza, Rosa E.
Silva, Roberto N.
Gutiérrez , Santiago 1965-
author Gomes, Eriston V.
author_facet Gomes, Eriston V.
Ulhoa, Cirano J.
Cardoza, Rosa E.
Silva, Roberto N.
Gutiérrez , Santiago 1965-
author_role author
author2 Ulhoa, Cirano J.
Cardoza, Rosa E.
Silva, Roberto N.
Gutiérrez , Santiago 1965-
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Microbiologia
Escuela de Ingeniería Agraria y Forestal
dc.subject.none.fl_str_mv Biología
Bioquímica
Trichoderma harzianum
Epl-1 protein
Botrytis cinerea
Fungus–plant interaction
Mycoparasitism
topic Biología
Bioquímica
Trichoderma harzianum
Epl-1 protein
Botrytis cinerea
Fungus–plant interaction
Mycoparasitism
description [EN] Several Trichoderma spp. are well known for their ability to: (i) act as important biocontrol agents against phytopathogenic fungi; (ii) function as biofertilizers; (iii) increase the tolerance of plants to biotic and abiotic stresses; and (iv) induce plant defense responses via the production and secretion of elicitor molecules. In this study, we analyzed the gene-regulation effects of Trichoderma harzianum Epl-1 protein during the interactions of mutant ∆epl-1 or wild-type T. harzianum strains with: (a) the phytopathogen Botrytis cinerea and (b) with tomato plants, on short (24 h hydroponic cultures) and long periods (4-weeks old plants) after Trichoderma inoculation. Our results indicate that T. harzianum Epl-1 protein affects the in vitro expression of B. cinerea virulence genes, especially those involved in the botrydial biosynthesis (BcBOT genes), during the mycoparasitism interaction. The tomato defense-related genes were also affected, indicating that Epl-1 is involved in the elicitation of the salicylic acid pathway. Moreover, Epl-1 also regulates the priming effect in host tomato plants and contributes to enhance the interaction with the host tomato plant during the early stage of root colonization
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.00880/full
https://hdl.handle.net/10612/19105
url https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2017.00880/full
https://hdl.handle.net/10612/19105
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nd/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404293194317824
score 15,300719

Similar Items