The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L
Endocrine-disrupting chemicals include natural and synthetic estrogens, such as 17α- ethynilestradiol (EE2 ), which can affect reproduction, growth and immunity. Estrogen signalling is mediated by nuclear or membrane estrogen receptors, such as the new G-protein-coupled estrogen receptor 1 (GPER1)....
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2021 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/313437 |
| Acceso en línea: | http://hdl.handle.net/10261/313437 |
| Access Level: | acceso abierto |
| Palabra clave: | Acuicultura EE2 Centro Oceanográfico de Murcia GPER1 body growth Muscle cellularity muscle growth regulator genes fish histology Animal growth Muscle fibers Endocrine disruptors |
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The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata LAyala, María DoloresGómez, VictoriaCabas, IsabelGarcía Hernández, María PilarChaves-Pozo, ElenaArizcun, MartaGarcía de la Serrana, DanielGil, FranciscoGarcía-Ayala, AlfonsaAcuiculturaEE2Centro Oceanográfico de MurciaGPER1body growthMuscle cellularitymuscle growth regulator genesfishhistologyAnimal growthMuscle fibersEndocrine disruptorsEndocrine-disrupting chemicals include natural and synthetic estrogens, such as 17α- ethynilestradiol (EE2 ), which can affect reproduction, growth and immunity. Estrogen signalling is mediated by nuclear or membrane estrogen receptors, such as the new G-protein-coupled estrogen receptor 1 (GPER1). The present work studies the effect of EE2 and G1 (an agonist of GPER1) on body and muscle parameters and growth-related genes of 54 two-year-old seabreams. The fish were fed a diet containing EE2 (EE2 group) and G1 (G1 group) for 45 days and then a diet without EE2 or G1 for 122 days. An untreated control group was also studied. At 45 days, the shortest body length was observed in the G1 group, while 79 and 122 days after the cessation of treatments, the shortest body growth was observed in the EE2 group. Hypertrophy of white fibers was higher in the EE2 and G1 groups than it was in the control group, whereas the opposite was the case with respect to hy- perplasia. Textural hardness showed a negative correlation with the size of white fibers. At the end of the experiment, all fish analyzed in the EE2 group showed a predominance of the gonadal ovarian area. In addition, the highest expression of the mafbx gene (upregulated in catabolic signals) and mstn2 (myogenesis negative regulator) was found in EE2 -exposed fish.SI202320232021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/313437reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#AGL2017-85978-C2-1-RCentro Oceanográfico de Murciawww.mdpi.com/1422-0067/22/23/13118/htminfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3134372026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L |
| title |
The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L |
| spellingShingle |
The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L Ayala, María Dolores Acuicultura EE2 Centro Oceanográfico de Murcia GPER1 body growth Muscle cellularity muscle growth regulator genes fish histology Animal growth Muscle fibers Endocrine disruptors |
| title_short |
The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L |
| title_full |
The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L |
| title_fullStr |
The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L |
| title_full_unstemmed |
The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L |
| title_sort |
The Effect of 17α-Ethynilestradiol and GPER1 Activation on Body and Muscle Growth, Muscle Composition and Growth-Related Gene Expression of Gilthead Seabream, Sparus aurata L |
| dc.creator.none.fl_str_mv |
Ayala, María Dolores Gómez, Victoria Cabas, Isabel García Hernández, María Pilar Chaves-Pozo, Elena Arizcun, Marta García de la Serrana, Daniel Gil, Francisco García-Ayala, Alfonsa |
| author |
Ayala, María Dolores |
| author_facet |
Ayala, María Dolores Gómez, Victoria Cabas, Isabel García Hernández, María Pilar Chaves-Pozo, Elena Arizcun, Marta García de la Serrana, Daniel Gil, Francisco García-Ayala, Alfonsa |
| author_role |
author |
| author2 |
Gómez, Victoria Cabas, Isabel García Hernández, María Pilar Chaves-Pozo, Elena Arizcun, Marta García de la Serrana, Daniel Gil, Francisco García-Ayala, Alfonsa |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Acuicultura EE2 Centro Oceanográfico de Murcia GPER1 body growth Muscle cellularity muscle growth regulator genes fish histology Animal growth Muscle fibers Endocrine disruptors |
| topic |
Acuicultura EE2 Centro Oceanográfico de Murcia GPER1 body growth Muscle cellularity muscle growth regulator genes fish histology Animal growth Muscle fibers Endocrine disruptors |
| description |
Endocrine-disrupting chemicals include natural and synthetic estrogens, such as 17α- ethynilestradiol (EE2 ), which can affect reproduction, growth and immunity. Estrogen signalling is mediated by nuclear or membrane estrogen receptors, such as the new G-protein-coupled estrogen receptor 1 (GPER1). The present work studies the effect of EE2 and G1 (an agonist of GPER1) on body and muscle parameters and growth-related genes of 54 two-year-old seabreams. The fish were fed a diet containing EE2 (EE2 group) and G1 (G1 group) for 45 days and then a diet without EE2 or G1 for 122 days. An untreated control group was also studied. At 45 days, the shortest body length was observed in the G1 group, while 79 and 122 days after the cessation of treatments, the shortest body growth was observed in the EE2 group. Hypertrophy of white fibers was higher in the EE2 and G1 groups than it was in the control group, whereas the opposite was the case with respect to hy- perplasia. Textural hardness showed a negative correlation with the size of white fibers. At the end of the experiment, all fish analyzed in the EE2 group showed a predominance of the gonadal ovarian area. In addition, the highest expression of the mafbx gene (upregulated in catabolic signals) and mstn2 (myogenesis negative regulator) was found in EE2 -exposed fish. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021 2023 2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/313437 |
| url |
http://hdl.handle.net/10261/313437 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# AGL2017-85978-C2-1-R Centro Oceanográfico de Murcia www.mdpi.com/1422-0067/22/23/13118/htm |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
| reponame_str |
DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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1869404286332436480 |
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15,811543 |