An Engineered extraplastidial pathway for carotenoid biofortification of leaves

Carotenoids are lipophilic plastidial isoprenoids highly valued as nutrients and natural pigments. A correct balance of chlorophylls and carotenoids is required for photosynthesis and therefore highly regulated, making carotenoid enrichment of green tissues challenging. Here we show that leaf carote...

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Autores: Andersen, Trine B., Llorente, Briardo, Morelli, Luca, Torres-Montilla, Salvador, Bordanaba-Florit, Guillermo, Espinosa, Fausto A., Rodríguez-Goberna, Maria Rosa, Campos Martínez, Narciso, Olmedilla-Alonso, Begoña, Llansola-Portoles, Manuel J., Pascal, Andrew A., Rodríguez-Concepción, Manuel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/175562
Acceso en línea:https://hdl.handle.net/2445/175562
Access Level:acceso abierto
Palabra clave:Carotenoides
Antioxidants
Biosíntesi
Fulles
Carotenoids
Biosynthesis
Leaves
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spelling An Engineered extraplastidial pathway for carotenoid biofortification of leavesAndersen, Trine B.Llorente, BriardoMorelli, LucaTorres-Montilla, SalvadorBordanaba-Florit, GuillermoEspinosa, Fausto A.Rodríguez-Goberna, Maria RosaCampos Martínez, NarcisoOlmedilla-Alonso, BegoñaLlansola-Portoles, Manuel J.Pascal, Andrew A.Rodríguez-Concepción, ManuelCarotenoidesAntioxidantsBiosíntesiFullesCarotenoidsAntioxidantsBiosynthesisLeavesCarotenoids are lipophilic plastidial isoprenoids highly valued as nutrients and natural pigments. A correct balance of chlorophylls and carotenoids is required for photosynthesis and therefore highly regulated, making carotenoid enrichment of green tissues challenging. Here we show that leaf carotenoid levels can be boosted through engineering their biosynthesis outside the chloroplast. Transient expression experiments in Nicotiana benthamiana leaves indicated that high extraplastidial production of carotenoids requires an enhanced supply of their isoprenoid precursors in the cytosol, which was achieved using a deregulated form of the main ratedetermining enzyme of the mevalonic acid (MVA) pathway. Constructs encoding bacterial enzymes were used to convert these MVA-derived precursors into carotenoid biosynthetic intermediates that do not normally accumulate in leaves, such as phytoene and lycopene. Cytosolic versions of these enzymes produced extraplastidial carotenoids at levels similar to those of total endogenous (i.e. chloroplast) carotenoids. Strategies to enhance the development of endomembrane structures and lipid bodies as potential extraplastidial carotenoid storage systems were not successful to further increase carotenoid contents. Phytoene was found to be more bioaccessible when accumulated outside plastids, whereas lycopene formed cytosolic crystalloids very similar to those found in the chromoplasts of ripe tomatoes. This extraplastidial production of phytoene and lycopene led to an increased antioxidant capacity of leaves. Finally, we demonstrate that our system can be adapted for the biofortification of leafy vegetables such as lettuce.John Wiley & Sons2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/175562Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1111/pbi.13526Plant Biotechnology Journal, 2020, vol. 19, p. 1-14https://doi.org/10.1111/pbi.13526info:eu-repo/grantAgreement/EC/H2020/713673cc-by (c) Andersen, Trine B. et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1755622026-05-27T06:46:51Z
dc.title.none.fl_str_mv An Engineered extraplastidial pathway for carotenoid biofortification of leaves
title An Engineered extraplastidial pathway for carotenoid biofortification of leaves
spellingShingle An Engineered extraplastidial pathway for carotenoid biofortification of leaves
Andersen, Trine B.
Carotenoides
Antioxidants
Biosíntesi
Fulles
Carotenoids
Antioxidants
Biosynthesis
Leaves
title_short An Engineered extraplastidial pathway for carotenoid biofortification of leaves
title_full An Engineered extraplastidial pathway for carotenoid biofortification of leaves
title_fullStr An Engineered extraplastidial pathway for carotenoid biofortification of leaves
title_full_unstemmed An Engineered extraplastidial pathway for carotenoid biofortification of leaves
title_sort An Engineered extraplastidial pathway for carotenoid biofortification of leaves
dc.creator.none.fl_str_mv Andersen, Trine B.
Llorente, Briardo
Morelli, Luca
Torres-Montilla, Salvador
Bordanaba-Florit, Guillermo
Espinosa, Fausto A.
Rodríguez-Goberna, Maria Rosa
Campos Martínez, Narciso
Olmedilla-Alonso, Begoña
Llansola-Portoles, Manuel J.
Pascal, Andrew A.
Rodríguez-Concepción, Manuel
author Andersen, Trine B.
author_facet Andersen, Trine B.
Llorente, Briardo
Morelli, Luca
Torres-Montilla, Salvador
Bordanaba-Florit, Guillermo
Espinosa, Fausto A.
Rodríguez-Goberna, Maria Rosa
Campos Martínez, Narciso
Olmedilla-Alonso, Begoña
Llansola-Portoles, Manuel J.
Pascal, Andrew A.
Rodríguez-Concepción, Manuel
author_role author
author2 Llorente, Briardo
Morelli, Luca
Torres-Montilla, Salvador
Bordanaba-Florit, Guillermo
Espinosa, Fausto A.
Rodríguez-Goberna, Maria Rosa
Campos Martínez, Narciso
Olmedilla-Alonso, Begoña
Llansola-Portoles, Manuel J.
Pascal, Andrew A.
Rodríguez-Concepción, Manuel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Carotenoides
Antioxidants
Biosíntesi
Fulles
Carotenoids
Antioxidants
Biosynthesis
Leaves
topic Carotenoides
Antioxidants
Biosíntesi
Fulles
Carotenoids
Antioxidants
Biosynthesis
Leaves
description Carotenoids are lipophilic plastidial isoprenoids highly valued as nutrients and natural pigments. A correct balance of chlorophylls and carotenoids is required for photosynthesis and therefore highly regulated, making carotenoid enrichment of green tissues challenging. Here we show that leaf carotenoid levels can be boosted through engineering their biosynthesis outside the chloroplast. Transient expression experiments in Nicotiana benthamiana leaves indicated that high extraplastidial production of carotenoids requires an enhanced supply of their isoprenoid precursors in the cytosol, which was achieved using a deregulated form of the main ratedetermining enzyme of the mevalonic acid (MVA) pathway. Constructs encoding bacterial enzymes were used to convert these MVA-derived precursors into carotenoid biosynthetic intermediates that do not normally accumulate in leaves, such as phytoene and lycopene. Cytosolic versions of these enzymes produced extraplastidial carotenoids at levels similar to those of total endogenous (i.e. chloroplast) carotenoids. Strategies to enhance the development of endomembrane structures and lipid bodies as potential extraplastidial carotenoid storage systems were not successful to further increase carotenoid contents. Phytoene was found to be more bioaccessible when accumulated outside plastids, whereas lycopene formed cytosolic crystalloids very similar to those found in the chromoplasts of ripe tomatoes. This extraplastidial production of phytoene and lycopene led to an increased antioxidant capacity of leaves. Finally, we demonstrate that our system can be adapted for the biofortification of leafy vegetables such as lettuce.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/175562
url https://hdl.handle.net/2445/175562
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1111/pbi.13526
Plant Biotechnology Journal, 2020, vol. 19, p. 1-14
https://doi.org/10.1111/pbi.13526
info:eu-repo/grantAgreement/EC/H2020/713673
dc.rights.none.fl_str_mv cc-by (c) Andersen, Trine B. et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Andersen, Trine B. et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404269815267328
score 15.300719

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