Inhibition of porcine viruses by different cell-targeted antiviral drugs

Antiviral compounds targeting cellular metabolism instead of virus components have become an interesting issue for preventing and controlling the spread of virus infection, either as sole treatment or as a complement of vaccination. Some of these compounds are involved in the control of lipid metabo...

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Detalhes bibliográficos
Autores: León, Patricia de, Bustos, Maria José, Torres, Elisa, Cañas-Arranz, Rodrigo, Sobrino Castelló, Francisco, Carrascosa, Ángel L.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/214916
Acesso em linha:http://hdl.handle.net/10261/214916
Access Level:acceso abierto
Palavra-chave:Virus
Porcine
Antiviral
Lauryl gallate
Valproic acid
Cerulenin
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spelling Inhibition of porcine viruses by different cell-targeted antiviral drugsLeón, Patricia deBustos, Maria JoséTorres, ElisaCañas-Arranz, RodrigoSobrino Castelló, FranciscoCarrascosa, Ángel L.VirusPorcineAntiviralLauryl gallateValproic acidCeruleninAntiviral compounds targeting cellular metabolism instead of virus components have become an interesting issue for preventing and controlling the spread of virus infection, either as sole treatment or as a complement of vaccination. Some of these compounds are involved in the control of lipid metabolism and/or membrane rearrangements. Here, we describe the effect of three of these cell-targeting antivirals: lauryl gallate (LG), valproic acid (VPA), and cerulenin (CRL) in the multiplication of viruses causing important porcine diseases. The results confirm the antiviral action in cultured cells of LG against African swine fever virus (ASFV), foot and mouth disease virus (FMDV), vesicular stomatitis virus (VSV), and swine vesicular disease virus (SVDV), as well as the inhibitory effect of VPA and CRL on ASFV infection. Other gallate esters have been also assayed for their inhibition of FMDV growth. The combined action of these antivirals has been also tested in ASFV infections, with some synergistic effects when LG and VPA were co-administered. Regarding the mode of action of the antivirals, experiments on the effect of the time of its addition in infected cell cultures indicated that the inhibition by VPA and CRL occurred at early times after ASFV infection, while LG inhibited a late step in FMDV infection. In all the cases, the presence of the antiviral reduced or abolished the induction of virus-specific proteins. Interestingly, LG also reduced mortality and FMDV load in a mouse model. The possible use of cell-targeted antivirals against porcine diseases is discussed.Spanish Ministerio de Ciencia e Innovación (2011-20E112), Ministerio de Economía y Competitividad (AGL2014-52395-C2-1-R and AGL2017-84097-C2-1-R), the European Community’s Seventh Framework Programme under grant agreement 311931-ASForce, Comunidad de Madrid co-financed with ECFEDER funds (S20149/ABI-2906-PLATESA; P2018/BAA-4370-PLATESA-CM), and by institutional grants from the Fundación Ramón Areces and Banco Santander UniversidadesMinisterio de Ciencia e Innovación (España)Ministerio de Economía y Competitividad (España)Comunidad de MadridFundación Ramón ArecesBanco SantanderConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/214916reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3389/fmicb.2019.01853Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2149162026-05-22T06:33:51Z
dc.title.none.fl_str_mv Inhibition of porcine viruses by different cell-targeted antiviral drugs
title Inhibition of porcine viruses by different cell-targeted antiviral drugs
spellingShingle Inhibition of porcine viruses by different cell-targeted antiviral drugs
León, Patricia de
Virus
Porcine
Antiviral
Lauryl gallate
Valproic acid
Cerulenin
title_short Inhibition of porcine viruses by different cell-targeted antiviral drugs
title_full Inhibition of porcine viruses by different cell-targeted antiviral drugs
title_fullStr Inhibition of porcine viruses by different cell-targeted antiviral drugs
title_full_unstemmed Inhibition of porcine viruses by different cell-targeted antiviral drugs
title_sort Inhibition of porcine viruses by different cell-targeted antiviral drugs
dc.creator.none.fl_str_mv León, Patricia de
Bustos, Maria José
Torres, Elisa
Cañas-Arranz, Rodrigo
Sobrino Castelló, Francisco
Carrascosa, Ángel L.
author León, Patricia de
author_facet León, Patricia de
Bustos, Maria José
Torres, Elisa
Cañas-Arranz, Rodrigo
Sobrino Castelló, Francisco
Carrascosa, Ángel L.
author_role author
author2 Bustos, Maria José
Torres, Elisa
Cañas-Arranz, Rodrigo
Sobrino Castelló, Francisco
Carrascosa, Ángel L.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Ministerio de Economía y Competitividad (España)
Comunidad de Madrid
Fundación Ramón Areces
Banco Santander
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Virus
Porcine
Antiviral
Lauryl gallate
Valproic acid
Cerulenin
topic Virus
Porcine
Antiviral
Lauryl gallate
Valproic acid
Cerulenin
description Antiviral compounds targeting cellular metabolism instead of virus components have become an interesting issue for preventing and controlling the spread of virus infection, either as sole treatment or as a complement of vaccination. Some of these compounds are involved in the control of lipid metabolism and/or membrane rearrangements. Here, we describe the effect of three of these cell-targeting antivirals: lauryl gallate (LG), valproic acid (VPA), and cerulenin (CRL) in the multiplication of viruses causing important porcine diseases. The results confirm the antiviral action in cultured cells of LG against African swine fever virus (ASFV), foot and mouth disease virus (FMDV), vesicular stomatitis virus (VSV), and swine vesicular disease virus (SVDV), as well as the inhibitory effect of VPA and CRL on ASFV infection. Other gallate esters have been also assayed for their inhibition of FMDV growth. The combined action of these antivirals has been also tested in ASFV infections, with some synergistic effects when LG and VPA were co-administered. Regarding the mode of action of the antivirals, experiments on the effect of the time of its addition in infected cell cultures indicated that the inhibition by VPA and CRL occurred at early times after ASFV infection, while LG inhibited a late step in FMDV infection. In all the cases, the presence of the antiviral reduced or abolished the induction of virus-specific proteins. Interestingly, LG also reduced mortality and FMDV load in a mouse model. The possible use of cell-targeted antivirals against porcine diseases is discussed.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/214916
url http://hdl.handle.net/10261/214916
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3389/fmicb.2019.01853
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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