Stability of Biomimetically Functionalised Alginate Microspheres as 3D Support in Cell Cultures
Alginate hydrogels can be used to develop a three-dimensional environment in which various cell types can be grown. Cross-linking the alginate chains using reversible ionic bonds opens up great possibilities for the encapsulation and subsequent release of cells or drugs. However, al-ginate also has...
| Autores: | , , , , , , |
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| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/187455 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/187455 |
| Access Level: | acceso abierto |
| Palabra clave: | Alginate Microspheres Stability Layer by layer Multiple myeloma CIENCIA DE LOS MATERIALES E INGENIERIA METALURGICA |
| Sumario: | Alginate hydrogels can be used to develop a three-dimensional environment in which various cell types can be grown. Cross-linking the alginate chains using reversible ionic bonds opens up great possibilities for the encapsulation and subsequent release of cells or drugs. However, al-ginate also has a drawback in that its structure is not very stable in a culture medium with cellu-lar activity. This work explored the stability of alginate microspheres functionalised by grafting specific biomolecules onto their surface to form microgels in which biomimetic microspheres surrounded the cells in the culture, reproducing the natural microenvironment. A study was made of the stability of the microgel in different typical culture media and the formation of polyelectrolyte multilayers containing polylysine and heparin. Multiple myeloma cell prolifer-ation in the culture was tested in a bioreactor under gentle agitation. |
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