A multiplexed immunochemical microarray for the determination of cardiovascular disease biomarkers
A fluorescence antibody microarray has been developed for the determination of relevant cardiovascular disease biomarkers for the analysis of human plasma samples. Recording characteristic protein molecular fingerprints to assess individual's states of health could allow diagnosis to go beyond...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:301971 |
| Acceso en línea: | https://ddd.uab.cat/record/301971 https://dx.doi.org/urn:doi:10.1007/s00604-023-06119-w |
| Access Level: | acceso abierto |
| Palabra clave: | Microarray Fluorescence detection Multiplexation Heart failure Acute myocardial infarction Infammation fuorescence Immunoassay |
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A multiplexed immunochemical microarray for the determination of cardiovascular disease biomarkersColom, GloriaHernandez-Albors, AlejandroBarallat, Jaume|||0000-0003-3493-5958Galan, AmparoBayés-Genís, Antoni|||0000-0002-3044-197XSalvador, Juan PabloMarco, María Pilar|||0000-0002-4064-1668MicroarrayFluorescence detectionMultiplexationHeart failureAcute myocardial infarctionInfammation fuorescenceImmunoassayA fluorescence antibody microarray has been developed for the determination of relevant cardiovascular disease biomarkers for the analysis of human plasma samples. Recording characteristic protein molecular fingerprints to assess individual's states of health could allow diagnosis to go beyond the simple identification of the disease, providing information on its stage or prognosis. Precisely, cardiovascular diseases (CVDs) are complex disorders which involve different degenerative processes encompassing a collection of biomarkers related to disease progression or stage. The novel approach that we propose is a fluorescent microarray chip has been developed accomplishing simultaneous determination of the most significant cardiac biomarkers in plasma aiming to determine the CVD status stage of the patient. As proof of concept, we have chosen five relevant biomarkers, C-reactive protein (CRP) as biomarker of inflammation, cystatin C (CysC) as biomarker of renal failure that is directly related with heart failure, cardiac troponin I (cTnI) as already established biomarker for cardiac damage, heart fatty acid binding protein as biomarker of ischemia (H-FABP), and finally, NT-proBNP (N-terminal pro-brain natriuretic peptide), a well-established heart failure biomarker. After the optimization of the multiplexed microarray, the assay allowed the simultaneous determination of 5 biomarkers in a buffer solution reaching LODs of 15 ± 5, 3 ± 1, 24 ± 3, 25 ± 3, and 3 ± 1 ng mL, for CRP, CysC, H-FABP, cTnI, and NT-proBNP, respectively. After solving the matrix effect, and demonstrating the accuracy for each biomarker, the chip was able to determine 24 samples per microarray chip. Then, the microarray has been used on a small pilot clinical study with 29 plasma samples from clinical patients which suffered different CVD and other related disorders. Results show the superior capability of the chip to provide clinical information related to the disease in terms of turnaround time (1 h 30 min total assay and measurement) and amount of information delivered in respect to reference technologies used in hospital laboratories (clinical analyzers). Despite the failure to detect c-TnI at the reported threshold, the microarray technology could be a powerful approach to diagnose the cardiovascular disease at early stage, monitor its progress, and eventually providing information about an eminent potential risk of suffering a myocardial infarction. The microarray chip here reported could be the starting point for achieving powerful multiplexed diagnostic technologies for the diagnosis of CVDs or any other pathology for which biomarkers have been identified at different stages of the disease. Graphical Abstract: [Figure not available: see fulltext.]. 22024-01-0120242024-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/301971https://dx.doi.org/urn:doi:10.1007/s00604-023-06119-wreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00408open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:3019712026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
A multiplexed immunochemical microarray for the determination of cardiovascular disease biomarkers |
| title |
A multiplexed immunochemical microarray for the determination of cardiovascular disease biomarkers |
| spellingShingle |
A multiplexed immunochemical microarray for the determination of cardiovascular disease biomarkers Colom, Gloria Microarray Fluorescence detection Multiplexation Heart failure Acute myocardial infarction Infammation fuorescence Immunoassay |
| title_short |
A multiplexed immunochemical microarray for the determination of cardiovascular disease biomarkers |
| title_full |
A multiplexed immunochemical microarray for the determination of cardiovascular disease biomarkers |
| title_fullStr |
A multiplexed immunochemical microarray for the determination of cardiovascular disease biomarkers |
| title_full_unstemmed |
A multiplexed immunochemical microarray for the determination of cardiovascular disease biomarkers |
| title_sort |
A multiplexed immunochemical microarray for the determination of cardiovascular disease biomarkers |
| dc.creator.none.fl_str_mv |
Colom, Gloria Hernandez-Albors, Alejandro Barallat, Jaume|||0000-0003-3493-5958 Galan, Amparo Bayés-Genís, Antoni|||0000-0002-3044-197X Salvador, Juan Pablo Marco, María Pilar|||0000-0002-4064-1668 |
| author |
Colom, Gloria |
| author_facet |
Colom, Gloria Hernandez-Albors, Alejandro Barallat, Jaume|||0000-0003-3493-5958 Galan, Amparo Bayés-Genís, Antoni|||0000-0002-3044-197X Salvador, Juan Pablo Marco, María Pilar|||0000-0002-4064-1668 |
| author_role |
author |
| author2 |
Hernandez-Albors, Alejandro Barallat, Jaume|||0000-0003-3493-5958 Galan, Amparo Bayés-Genís, Antoni|||0000-0002-3044-197X Salvador, Juan Pablo Marco, María Pilar|||0000-0002-4064-1668 |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Microarray Fluorescence detection Multiplexation Heart failure Acute myocardial infarction Infammation fuorescence Immunoassay |
| topic |
Microarray Fluorescence detection Multiplexation Heart failure Acute myocardial infarction Infammation fuorescence Immunoassay |
| description |
A fluorescence antibody microarray has been developed for the determination of relevant cardiovascular disease biomarkers for the analysis of human plasma samples. Recording characteristic protein molecular fingerprints to assess individual's states of health could allow diagnosis to go beyond the simple identification of the disease, providing information on its stage or prognosis. Precisely, cardiovascular diseases (CVDs) are complex disorders which involve different degenerative processes encompassing a collection of biomarkers related to disease progression or stage. The novel approach that we propose is a fluorescent microarray chip has been developed accomplishing simultaneous determination of the most significant cardiac biomarkers in plasma aiming to determine the CVD status stage of the patient. As proof of concept, we have chosen five relevant biomarkers, C-reactive protein (CRP) as biomarker of inflammation, cystatin C (CysC) as biomarker of renal failure that is directly related with heart failure, cardiac troponin I (cTnI) as already established biomarker for cardiac damage, heart fatty acid binding protein as biomarker of ischemia (H-FABP), and finally, NT-proBNP (N-terminal pro-brain natriuretic peptide), a well-established heart failure biomarker. After the optimization of the multiplexed microarray, the assay allowed the simultaneous determination of 5 biomarkers in a buffer solution reaching LODs of 15 ± 5, 3 ± 1, 24 ± 3, 25 ± 3, and 3 ± 1 ng mL, for CRP, CysC, H-FABP, cTnI, and NT-proBNP, respectively. After solving the matrix effect, and demonstrating the accuracy for each biomarker, the chip was able to determine 24 samples per microarray chip. Then, the microarray has been used on a small pilot clinical study with 29 plasma samples from clinical patients which suffered different CVD and other related disorders. Results show the superior capability of the chip to provide clinical information related to the disease in terms of turnaround time (1 h 30 min total assay and measurement) and amount of information delivered in respect to reference technologies used in hospital laboratories (clinical analyzers). Despite the failure to detect c-TnI at the reported threshold, the microarray technology could be a powerful approach to diagnose the cardiovascular disease at early stage, monitor its progress, and eventually providing information about an eminent potential risk of suffering a myocardial infarction. The microarray chip here reported could be the starting point for achieving powerful multiplexed diagnostic technologies for the diagnosis of CVDs or any other pathology for which biomarkers have been identified at different stages of the disease. Graphical Abstract: [Figure not available: see fulltext.]. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2 2024-01-01 2024 2024-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/301971 https://dx.doi.org/urn:doi:10.1007/s00604-023-06119-w |
| url |
https://ddd.uab.cat/record/301971 https://dx.doi.org/urn:doi:10.1007/s00604-023-06119-w |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2021/SGR-00408 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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Universitat Autònoma de Barcelona |
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