A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values

Pepsin is a protease used in many different applications, and in many instances, it is utilized in an immobilized form to prevent contamination of the reaction product. This enzyme has two peculiarities that make its immobilization complex. The first one is related to the poor presence of primary am...

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Autores: Morellon-Sterling, Roberto, Tavano, Olga, Bolívar Bolívar, Juan Manuel, Berenguer-Murcia, Ángel, Vela-Gutiérrez, Gilber, Sabir, Jamal, Tacias-Pascacio, Veymar, Fernandez-Lafuente, Roberto
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/101578
Acceso en línea:https://hdl.handle.net/20.500.14352/101578
Access Level:acceso abierto
Palabra clave:66.0
620
Multi-point covalent immobilization
Ion exchange
Glutaraldehyde versatility
Ingeniería química
Química industrial
Bioquímica (Química)
2302 Bioquímica
3302 Tecnología Bioquímica
3303 Ingeniería y Tecnología Químicas
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network_acronym_str ES
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repository_id_str
spelling A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH valuesMorellon-Sterling, RobertoTavano, OlgaBolívar Bolívar, Juan ManuelBerenguer-Murcia, ÁngelVela-Gutiérrez, GilberSabir, JamalTacias-Pascacio, VeymarFernandez-Lafuente, Roberto66.0620Multi-point covalent immobilizationIon exchangeGlutaraldehyde versatilityIngeniería químicaQuímica industrialBioquímica (Química)2302 Bioquímica3302 Tecnología Bioquímica3303 Ingeniería y Tecnología QuímicasPepsin is a protease used in many different applications, and in many instances, it is utilized in an immobilized form to prevent contamination of the reaction product. This enzyme has two peculiarities that make its immobilization complex. The first one is related to the poor presence of primary amino groups on its surface (just one Lys and the terminal amino group). The second one is its poor stability at alkaline pH values. Both features make the immobilization of this enzyme to be considered a complicated goal, as most of the immobilization protocols utilize primary amino groups for immobilization. This review presents some of the attempts to get immobilized pepsin biocatalyst and their applications. The high density of anionic groups (Asp and Glu) make the anion exchange of the enzyme simpler, but this makes many of the strategies utilized to immobilize the enzyme (e.g., amino-glutaraldehyde supports) more related to a mixed ion exchange/hydrophobic adsorption than to real covalent immobilization. Finally, we propose some possibilities that can permit not only the covalent immobilization of this enzyme, but also their stabilization via multipoint covalent attachment.ElsevierUniversidad Complutense de Madrid20222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/101578reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 CTQ2017-86170-R DISEÑO DE ESTRATEGIAS PARA LA PRODUCCION DE CATALIZADORES CON ENZIMAS COINMOVILIZADAS Y SU EMPLEO EN REACCIONES EN CASCADAAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-095291-B-I00 APROVECHAMIENTO MAS EFICIENTE DE BIOMASA LIGNOCELULOSICA PARA LA OBTENCION DE MATERIALES CARBONOSOS DE ALTAS PRESTACIONES Y PRODUCTOS QUIMICOSopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1015782026-06-02T12:44:21Z
dc.title.none.fl_str_mv A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values
title A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values
spellingShingle A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values
Morellon-Sterling, Roberto
66.0
620
Multi-point covalent immobilization
Ion exchange
Glutaraldehyde versatility
Ingeniería química
Química industrial
Bioquímica (Química)
2302 Bioquímica
3302 Tecnología Bioquímica
3303 Ingeniería y Tecnología Químicas
title_short A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values
title_full A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values
title_fullStr A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values
title_full_unstemmed A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values
title_sort A review on the immobilization of pepsin: A Lys-poor enzyme that is unstable at alkaline pH values
dc.creator.none.fl_str_mv Morellon-Sterling, Roberto
Tavano, Olga
Bolívar Bolívar, Juan Manuel
Berenguer-Murcia, Ángel
Vela-Gutiérrez, Gilber
Sabir, Jamal
Tacias-Pascacio, Veymar
Fernandez-Lafuente, Roberto
author Morellon-Sterling, Roberto
author_facet Morellon-Sterling, Roberto
Tavano, Olga
Bolívar Bolívar, Juan Manuel
Berenguer-Murcia, Ángel
Vela-Gutiérrez, Gilber
Sabir, Jamal
Tacias-Pascacio, Veymar
Fernandez-Lafuente, Roberto
author_role author
author2 Tavano, Olga
Bolívar Bolívar, Juan Manuel
Berenguer-Murcia, Ángel
Vela-Gutiérrez, Gilber
Sabir, Jamal
Tacias-Pascacio, Veymar
Fernandez-Lafuente, Roberto
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 66.0
620
Multi-point covalent immobilization
Ion exchange
Glutaraldehyde versatility
Ingeniería química
Química industrial
Bioquímica (Química)
2302 Bioquímica
3302 Tecnología Bioquímica
3303 Ingeniería y Tecnología Químicas
topic 66.0
620
Multi-point covalent immobilization
Ion exchange
Glutaraldehyde versatility
Ingeniería química
Química industrial
Bioquímica (Química)
2302 Bioquímica
3302 Tecnología Bioquímica
3303 Ingeniería y Tecnología Químicas
description Pepsin is a protease used in many different applications, and in many instances, it is utilized in an immobilized form to prevent contamination of the reaction product. This enzyme has two peculiarities that make its immobilization complex. The first one is related to the poor presence of primary amino groups on its surface (just one Lys and the terminal amino group). The second one is its poor stability at alkaline pH values. Both features make the immobilization of this enzyme to be considered a complicated goal, as most of the immobilization protocols utilize primary amino groups for immobilization. This review presents some of the attempts to get immobilized pepsin biocatalyst and their applications. The high density of anionic groups (Asp and Glu) make the anion exchange of the enzyme simpler, but this makes many of the strategies utilized to immobilize the enzyme (e.g., amino-glutaraldehyde supports) more related to a mixed ion exchange/hydrophobic adsorption than to real covalent immobilization. Finally, we propose some possibilities that can permit not only the covalent immobilization of this enzyme, but also their stabilization via multipoint covalent attachment.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/101578
url https://hdl.handle.net/20.500.14352/101578
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 CTQ2017-86170-R DISEÑO DE ESTRATEGIAS PARA LA PRODUCCION DE CATALIZADORES CON ENZIMAS COINMOVILIZADAS Y SU EMPLEO EN REACCIONES EN CASCADA
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 RTI2018-095291-B-I00 APROVECHAMIENTO MAS EFICIENTE DE BIOMASA LIGNOCELULOSICA PARA LA OBTENCION DE MATERIALES CARBONOSOS DE ALTAS PRESTACIONES Y PRODUCTOS QUIMICOS
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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