Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)

Fuentes Cordero, Juan Luis; Huss, Volker A. R.; Montero Lobato, Zaida; Torronteras Santiago, Rafael; Cuaresma Franco, María; Garbayo Nores, Inés; Vílchez Lobato, Carlos

Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)

The genus Coccomyxa comprises green microalgae, which can be found worldwide in remarkably versatile aquatic and terrestrial ecosystems including symbiotic associations with a number of different hosts. In this study, we describe a new species, Coccomyxa onubensis, based on 18S and ITS ribosomal DNA...

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Autores:	Fuentes Cordero, Juan Luis, Huss, Volker A. R., Montero Lobato, Zaida, Torronteras Santiago, Rafael, Cuaresma Franco, María, Garbayo Nores, Inés, Vílchez Lobato, Carlos
Formato:	artículo
Fecha de publicación:	2016
País:	España
Recursos:	Universidad de Huelva (UHU)
Repositorio:	Arias Montano. Repositorio Institucional de la Universidad de Huelva
Idioma:	inglés
OAI Identifier:	oai:ariasmontano.uhu.es:10272/24958
Acesso em linha:	https://hdl.handle.net/10272/24958
Access Level:	acceso abierto
Palavra-chave:	Coccomyxa Green algae Phylogeny Acidotolerance Halotolerance Biotechnology 3302 Tecnología Bioquímica 2302 Bioquímica

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spelling	Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)Fuentes Cordero, Juan LuisHuss, Volker A. R.Montero Lobato, ZaidaTorronteras Santiago, RafaelCuaresma Franco, MaríaGarbayo Nores, InésVílchez Lobato, CarlosCoccomyxaGreen algaePhylogenyAcidotoleranceHalotoleranceBiotechnology3302 Tecnología Bioquímica2302 BioquímicaThe genus Coccomyxa comprises green microalgae, which can be found worldwide in remarkably versatile aquatic and terrestrial ecosystems including symbiotic associations with a number of different hosts. In this study, we describe a new species, Coccomyxa onubensis, based on 18S and ITS ribosomal DNA (rDNA) sequence data. Coccomyxa onubensis was isolated from acidic water, and its ability to adapt to a wide range of acidic and alkaline pH values and to high salinity was analyzed. The long-termadaptation capacity of the microalga to such extreme conditions was evaluated by performing continuous repeated batches at selected salt concentrations and pH values. Adapted cultures of C. onubensis were found to yield high biomass productivities from pH 2.5 to 9, with maximum yields at acidic pH between 2.5 and 4.5. Moreover, C. onubensis was also found to adapt to salinities as high as 0.5 M NaCl, reaching biomass productivities that were similar to those of control cultures. Ultrastructural analysis by transmission electron microscopy of C. onubensis cells adapted to high salinity showed a robust response to hyperosmotic shock. Thus, C. onubensis was found to be acidotolerant and halotolerant. High biomass productivity over a wide range of pH and salinities denotes C. onubensis as an interesting candidate for various biotechnological applications including outdoor biomass production.20162016-06-2120162016-06-21journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10272/24958reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelvainstname:Universidad de Huelva (UHU)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttp://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:ariasmontano.uhu.es:10272/249582026-06-02T14:58:11Z
dc.title.none.fl_str_mv	Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)
title	Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)
spellingShingle	Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae) Fuentes Cordero, Juan Luis Coccomyxa Green algae Phylogeny Acidotolerance Halotolerance Biotechnology 3302 Tecnología Bioquímica 2302 Bioquímica
title_short	Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)
title_full	Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)
title_fullStr	Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)
title_full_unstemmed	Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)
title_sort	Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)
dc.creator.none.fl_str_mv	Fuentes Cordero, Juan Luis Huss, Volker A. R. Montero Lobato, Zaida Torronteras Santiago, Rafael Cuaresma Franco, María Garbayo Nores, Inés Vílchez Lobato, Carlos
author	Fuentes Cordero, Juan Luis
author_facet	Fuentes Cordero, Juan Luis Huss, Volker A. R. Montero Lobato, Zaida Torronteras Santiago, Rafael Cuaresma Franco, María Garbayo Nores, Inés Vílchez Lobato, Carlos
author_role	author
author2	Huss, Volker A. R. Montero Lobato, Zaida Torronteras Santiago, Rafael Cuaresma Franco, María Garbayo Nores, Inés Vílchez Lobato, Carlos
author2_role	author author author author author author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv	Coccomyxa Green algae Phylogeny Acidotolerance Halotolerance Biotechnology 3302 Tecnología Bioquímica 2302 Bioquímica
topic	Coccomyxa Green algae Phylogeny Acidotolerance Halotolerance Biotechnology 3302 Tecnología Bioquímica 2302 Bioquímica
description	The genus Coccomyxa comprises green microalgae, which can be found worldwide in remarkably versatile aquatic and terrestrial ecosystems including symbiotic associations with a number of different hosts. In this study, we describe a new species, Coccomyxa onubensis, based on 18S and ITS ribosomal DNA (rDNA) sequence data. Coccomyxa onubensis was isolated from acidic water, and its ability to adapt to a wide range of acidic and alkaline pH values and to high salinity was analyzed. The long-termadaptation capacity of the microalga to such extreme conditions was evaluated by performing continuous repeated batches at selected salt concentrations and pH values. Adapted cultures of C. onubensis were found to yield high biomass productivities from pH 2.5 to 9, with maximum yields at acidic pH between 2.5 and 4.5. Moreover, C. onubensis was also found to adapt to salinities as high as 0.5 M NaCl, reaching biomass productivities that were similar to those of control cultures. Ultrastructural analysis by transmission electron microscopy of C. onubensis cells adapted to high salinity showed a robust response to hyperosmotic shock. Thus, C. onubensis was found to be acidotolerant and halotolerant. High biomass productivity over a wide range of pH and salinities denotes C. onubensis as an interesting candidate for various biotechnological applications including outdoor biomass production.
publishDate	2016
dc.date.none.fl_str_mv	2016 2016-06-21 2016 2016-06-21
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501 AM http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://hdl.handle.net/10272/24958
url	https://hdl.handle.net/10272/24958
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Arias Montano. Repositorio Institucional de la Universidad de Huelva instname:Universidad de Huelva (UHU)
instname_str	Universidad de Huelva (UHU)
reponame_str	Arias Montano. Repositorio Institucional de la Universidad de Huelva
collection	Arias Montano. Repositorio Institucional de la Universidad de Huelva
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15.811543

Phylogenetic characterization and morphological and physiological aspects of a novel acidotolerant and halotolerant microalga Coccomyxa onubensis sp. nov. (Chlorophyta, Trebouxiophyceae)

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