Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4

The spatiotemporal control of meristem identity is critical for determining inflorescence architecture, and thus yield, of cereal plants. However, the precise mechanisms underlying inflorescence and spikelet meristem determinacy in cereals are still largely unclear. We have generated loss-of-functio...

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Autores: Zhu, Wanwan, Yang, Liu, Wu, Di, Meng, Qingcai, Deng, Xiao, Huang, Guoqiang, Zhang, Jiao, Chen, Xiaofei, Ferrándiz, Cristina, Liang, Wanqi, Dreni, Ludovico, Zhang, Dabing
Tipo de documento: artigo
Data de publicação:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/304740
Acesso em linha:http://hdl.handle.net/10261/304740
https://api.elsevier.com/content/abstract/scopus_id/85120158606
Access Level:Acceso aberto
Palavra-chave:OsMADS34
OsMADS5
RCN4
TERMINAL FLOWER1
MADS-box
inflorescence architecture and branching
meristem
rice (Oryza sativa)
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oai_identifier_str oai:digital.csic.es:10261/304740
network_acronym_str ES
network_name_str España
repository_id_str
spelling Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4Zhu, WanwanYang, LiuWu, DiMeng, QingcaiDeng, XiaoHuang, GuoqiangZhang, JiaoChen, XiaofeiFerrándiz, CristinaLiang, WanqiDreni, LudovicoZhang, DabingOsMADS34OsMADS5RCN4TERMINAL FLOWER1MADS-boxinflorescence architecture and branchingmeristemrice (Oryza sativa)The spatiotemporal control of meristem identity is critical for determining inflorescence architecture, and thus yield, of cereal plants. However, the precise mechanisms underlying inflorescence and spikelet meristem determinacy in cereals are still largely unclear. We have generated loss-of-function and overexpression mutants of the paralogous OsMADS5 and OsMADS34 genes in rice (Oryza sativa), and analysed their panicle phenotypes. Using chromatin immunoprecipitation, electrophoretic mobility-shift and dual-luciferase assays, we have also identified RICE CENTRORADIALIS 4 (RCN4), a TFL1-like gene, as a direct downstream target of both OsMADS proteins, and have analysed RCN4 mutants. The osmads5 osmads34 mutant lines had significantly enhanced panicle branching with increased secondary, and even tertiary and quaternary, branches, compared to wild-type (WT) and osmads34 plants. The osmads34 mutant phenotype could largely be rescued by also knocking out RCN4. Moreover, transgenic panicles overexpressing RCN4 had significantly increased branching, and initiated development of c. 7× more spikelets than WT. Our results reveal a role for OsMADS5 in panicle development, and show that OsMADS5 and OsMADS34 play similar functions in limiting branching and promoting the transition to spikelet meristem identity, in part by repressing RCN4 expression. These findings provide new insights to better understand the molecular regulation of rice inflorescence architecture.Peer reviewed0000-0002-3545-00900000-0003-4402-22710000-0003-1101-11700000-0002-6103-57040000-0002-9106-21560000-0002-2460-10680000-0002-9938-57930000-0002-2059-84200000-0003-3181-9812Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/304740https://api.elsevier.com/content/abstract/scopus_id/85120158606reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe New phytologistSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3047402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4
title Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4
spellingShingle Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4
Zhu, Wanwan
OsMADS34
OsMADS5
RCN4
TERMINAL FLOWER1
MADS-box
inflorescence architecture and branching
meristem
rice (Oryza sativa)
title_short Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4
title_full Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4
title_fullStr Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4
title_full_unstemmed Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4
title_sort Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4
dc.creator.none.fl_str_mv Zhu, Wanwan
Yang, Liu
Wu, Di
Meng, Qingcai
Deng, Xiao
Huang, Guoqiang
Zhang, Jiao
Chen, Xiaofei
Ferrándiz, Cristina
Liang, Wanqi
Dreni, Ludovico
Zhang, Dabing
author Zhu, Wanwan
author_facet Zhu, Wanwan
Yang, Liu
Wu, Di
Meng, Qingcai
Deng, Xiao
Huang, Guoqiang
Zhang, Jiao
Chen, Xiaofei
Ferrándiz, Cristina
Liang, Wanqi
Dreni, Ludovico
Zhang, Dabing
author_role author
author2 Yang, Liu
Wu, Di
Meng, Qingcai
Deng, Xiao
Huang, Guoqiang
Zhang, Jiao
Chen, Xiaofei
Ferrándiz, Cristina
Liang, Wanqi
Dreni, Ludovico
Zhang, Dabing
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv 0000-0002-3545-0090
0000-0003-4402-2271
0000-0003-1101-1170
0000-0002-6103-5704
0000-0002-9106-2156
0000-0002-2460-1068
0000-0002-9938-5793
0000-0002-2059-8420
0000-0003-3181-9812
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv OsMADS34
OsMADS5
RCN4
TERMINAL FLOWER1
MADS-box
inflorescence architecture and branching
meristem
rice (Oryza sativa)
topic OsMADS34
OsMADS5
RCN4
TERMINAL FLOWER1
MADS-box
inflorescence architecture and branching
meristem
rice (Oryza sativa)
description The spatiotemporal control of meristem identity is critical for determining inflorescence architecture, and thus yield, of cereal plants. However, the precise mechanisms underlying inflorescence and spikelet meristem determinacy in cereals are still largely unclear. We have generated loss-of-function and overexpression mutants of the paralogous OsMADS5 and OsMADS34 genes in rice (Oryza sativa), and analysed their panicle phenotypes. Using chromatin immunoprecipitation, electrophoretic mobility-shift and dual-luciferase assays, we have also identified RICE CENTRORADIALIS 4 (RCN4), a TFL1-like gene, as a direct downstream target of both OsMADS proteins, and have analysed RCN4 mutants. The osmads5 osmads34 mutant lines had significantly enhanced panicle branching with increased secondary, and even tertiary and quaternary, branches, compared to wild-type (WT) and osmads34 plants. The osmads34 mutant phenotype could largely be rescued by also knocking out RCN4. Moreover, transgenic panicles overexpressing RCN4 had significantly increased branching, and initiated development of c. 7× more spikelets than WT. Our results reveal a role for OsMADS5 in panicle development, and show that OsMADS5 and OsMADS34 play similar functions in limiting branching and promoting the transition to spikelet meristem identity, in part by repressing RCN4 expression. These findings provide new insights to better understand the molecular regulation of rice inflorescence architecture.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/304740
https://api.elsevier.com/content/abstract/scopus_id/85120158606
url http://hdl.handle.net/10261/304740
https://api.elsevier.com/content/abstract/scopus_id/85120158606
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The New phytologist
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404250056949760
score 15,811543

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