Effectiveness of direct laser interference patterning and peptide immobilization on endothelial cell migration for cardio-vascular applications: an in vitro study

Endothelial coverage of an exposed cardiovascular stent surface leads to the occurrence of restenosis and late-stent thrombosis several months after implantation. To overcome this difficulty, modification of stent surfaces with topographical or biochemical features may be performed to increase endot...

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Autores: Schieber, Romain, Mas Moruno, Carlos|||0000-0001-8337-0872, Lasserre, Federico, Roa Rovira, Joan Josep|||0000-0002-7440-0766, Ginebra Molins, Maria Pau|||0000-0002-4700-5621, Mücklich, Frank T., Pegueroles Neyra, Marta|||0000-0002-7895-8337
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/370825
Acceso en línea:https://hdl.handle.net/2117/370825
https://dx.doi.org/10.3390/nano12071217
Access Level:acceso abierto
Palabra clave:Nanotechnology
Direct laser interference patterning (DLIP)
Cobalt-chromium alloy
Biofunctionalization
Cell adhesive peptides
Endothelial cell migration
Nanotecnologia
Àrees temàtiques de la UPC::Enginyeria dels materials
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spelling Effectiveness of direct laser interference patterning and peptide immobilization on endothelial cell migration for cardio-vascular applications: an in vitro studySchieber, RomainMas Moruno, Carlos|||0000-0001-8337-0872Lasserre, FedericoRoa Rovira, Joan Josep|||0000-0002-7440-0766Ginebra Molins, Maria Pau|||0000-0002-4700-5621Mücklich, Frank T.Pegueroles Neyra, Marta|||0000-0002-7895-8337NanotechnologyDirect laser interference patterning (DLIP)Cobalt-chromium alloyBiofunctionalizationCell adhesive peptidesEndothelial cell migrationNanotecnologiaÀrees temàtiques de la UPC::Enginyeria dels materialsEndothelial coverage of an exposed cardiovascular stent surface leads to the occurrence of restenosis and late-stent thrombosis several months after implantation. To overcome this difficulty, modification of stent surfaces with topographical or biochemical features may be performed to increase endothelial cells’ (ECs) adhesion and/or migration. This work combines both strategies on cobalt-chromium (CoCr) alloy and studies the potential synergistic effect of linear patterned surfaces that are obtained by direct laser interference patterning (DLIP), coupled with the use of Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) peptides. An extensive characterization of the modified surfaces was performed by using AFM, XPS, surface charge, electrochemical analysis and fluorescent methods. The biological response was studied in terms of EC adhesion, migration and proliferation assays. CoCr surfaces were successfully patterned with a periodicity of 10 µm and two different depths, D (˜79 and 762 nm). RGD and YIGSR were immobilized on the surfaces by CPTES silanization. Early EC adhesion was increased on the peptide-functionalized surfaces, especially for YIGSR compared to RGD. High-depth patterns generated 80% of ECs’ alignment within the topographical lines and enhanced EC migration. It is noteworthy that the combined use of the two strategies synergistically accelerated the ECs’ migration and proliferation, proving the potential of this strategy to enhance stent endothelialization.Peer Reviewed20222022-04-0120222022-07-21journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/370825https://dx.doi.org/10.3390/nano12071217reponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3708252026-05-27T15:37:01Z
dc.title.none.fl_str_mv Effectiveness of direct laser interference patterning and peptide immobilization on endothelial cell migration for cardio-vascular applications: an in vitro study
title Effectiveness of direct laser interference patterning and peptide immobilization on endothelial cell migration for cardio-vascular applications: an in vitro study
spellingShingle Effectiveness of direct laser interference patterning and peptide immobilization on endothelial cell migration for cardio-vascular applications: an in vitro study
Schieber, Romain
Nanotechnology
Direct laser interference patterning (DLIP)
Cobalt-chromium alloy
Biofunctionalization
Cell adhesive peptides
Endothelial cell migration
Nanotecnologia
Àrees temàtiques de la UPC::Enginyeria dels materials
title_short Effectiveness of direct laser interference patterning and peptide immobilization on endothelial cell migration for cardio-vascular applications: an in vitro study
title_full Effectiveness of direct laser interference patterning and peptide immobilization on endothelial cell migration for cardio-vascular applications: an in vitro study
title_fullStr Effectiveness of direct laser interference patterning and peptide immobilization on endothelial cell migration for cardio-vascular applications: an in vitro study
title_full_unstemmed Effectiveness of direct laser interference patterning and peptide immobilization on endothelial cell migration for cardio-vascular applications: an in vitro study
title_sort Effectiveness of direct laser interference patterning and peptide immobilization on endothelial cell migration for cardio-vascular applications: an in vitro study
dc.creator.none.fl_str_mv Schieber, Romain
Mas Moruno, Carlos|||0000-0001-8337-0872
Lasserre, Federico
Roa Rovira, Joan Josep|||0000-0002-7440-0766
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Mücklich, Frank T.
Pegueroles Neyra, Marta|||0000-0002-7895-8337
author Schieber, Romain
author_facet Schieber, Romain
Mas Moruno, Carlos|||0000-0001-8337-0872
Lasserre, Federico
Roa Rovira, Joan Josep|||0000-0002-7440-0766
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Mücklich, Frank T.
Pegueroles Neyra, Marta|||0000-0002-7895-8337
author_role author
author2 Mas Moruno, Carlos|||0000-0001-8337-0872
Lasserre, Federico
Roa Rovira, Joan Josep|||0000-0002-7440-0766
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Mücklich, Frank T.
Pegueroles Neyra, Marta|||0000-0002-7895-8337
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Nanotechnology
Direct laser interference patterning (DLIP)
Cobalt-chromium alloy
Biofunctionalization
Cell adhesive peptides
Endothelial cell migration
Nanotecnologia
Àrees temàtiques de la UPC::Enginyeria dels materials
topic Nanotechnology
Direct laser interference patterning (DLIP)
Cobalt-chromium alloy
Biofunctionalization
Cell adhesive peptides
Endothelial cell migration
Nanotecnologia
Àrees temàtiques de la UPC::Enginyeria dels materials
description Endothelial coverage of an exposed cardiovascular stent surface leads to the occurrence of restenosis and late-stent thrombosis several months after implantation. To overcome this difficulty, modification of stent surfaces with topographical or biochemical features may be performed to increase endothelial cells’ (ECs) adhesion and/or migration. This work combines both strategies on cobalt-chromium (CoCr) alloy and studies the potential synergistic effect of linear patterned surfaces that are obtained by direct laser interference patterning (DLIP), coupled with the use of Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) peptides. An extensive characterization of the modified surfaces was performed by using AFM, XPS, surface charge, electrochemical analysis and fluorescent methods. The biological response was studied in terms of EC adhesion, migration and proliferation assays. CoCr surfaces were successfully patterned with a periodicity of 10 µm and two different depths, D (˜79 and 762 nm). RGD and YIGSR were immobilized on the surfaces by CPTES silanization. Early EC adhesion was increased on the peptide-functionalized surfaces, especially for YIGSR compared to RGD. High-depth patterns generated 80% of ECs’ alignment within the topographical lines and enhanced EC migration. It is noteworthy that the combined use of the two strategies synergistically accelerated the ECs’ migration and proliferation, proving the potential of this strategy to enhance stent endothelialization.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-04-01
2022
2022-07-21
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/370825
https://dx.doi.org/10.3390/nano12071217
url https://hdl.handle.net/2117/370825
https://dx.doi.org/10.3390/nano12071217
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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