Molecular characterization of Tunisian strains of Erwinia amylovora

The present study focused on the molecular characterization of a collection of Erwinia amylovora isolates recovered from different outbreaks in Tunisia between 2012 and 2014. Analysis of 54 isolates, including the reference type strain CFBP 1430, revealed that all Tunisian isolates produced the expe...

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Detalhes bibliográficos
Autores: Dardouri, Sana, Chehimi, Sonia, Murillo Martínez, Jesús, Hajlaoui, Mohamed Rabeh
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2017
País:España
Recursos:Universidad Pública de Navarra
Repositório:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/25081
Acesso em linha:https://hdl.handle.net/2454/25081
Access Level:Acceso aberto
Palavra-chave:Fire blight
Erwinia amylovora
PCR
CRIS- PR
VNTR primers
Fingerprinting
Epidemiology
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spelling Molecular characterization of Tunisian strains of Erwinia amylovoraDardouri, SanaChehimi, SoniaMurillo Martínez, JesúsHajlaoui, Mohamed RabehFire blightErwinia amylovoraPCRCRIS- PRVNTR primersFingerprintingEpidemiologyThe present study focused on the molecular characterization of a collection of Erwinia amylovora isolates recovered from different outbreaks in Tunisia between 2012 and 2014. Analysis of 54 isolates, including the reference type strain CFBP 1430, revealed that all Tunisian isolates produced the expected amplicons with diverse primer pairs routinely used for molecular diagnostics of E. amylovora. We also evaluated the genetic variability of these isolates by PCR fingerprinting, using specific primers for clustered regularly interspaced short palindromic repeats (CRISPRs) and for variable number of tandem repeats (VNTR) sequences. For the first method, our results revealed that all the primers used, except those for CRISPR3, which produced an identical amplicon for all isolates, showed some variability among Tunisian isolates. For the second method, forty-nine isolates showed the same fingerprint patterns as the reference type strain CFBP 1430 with all the primers used, whereas four of the isolates showed very divergent patterns. These results suggest that there has been a main introduction of European-type isolates in Tunisia, and possibly a few mutations or other independent introductions of the pathogen. Additionally, these results indicate that PCR fingerprinting using VNTR markers is a most useful tool for discriminating among E. amylovora strains and for their identification in epidemiological studies.Italian Phytopathological SocietyProducción AgrariaNekazaritza Ekoizpena2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/25081reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésCreative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0)https://creativecommons.org/licenses/by-sa/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/250812026-06-17T12:41:47Z
dc.title.none.fl_str_mv Molecular characterization of Tunisian strains of Erwinia amylovora
title Molecular characterization of Tunisian strains of Erwinia amylovora
spellingShingle Molecular characterization of Tunisian strains of Erwinia amylovora
Dardouri, Sana
Fire blight
Erwinia amylovora
PCR
CRIS- PR
VNTR primers
Fingerprinting
Epidemiology
title_short Molecular characterization of Tunisian strains of Erwinia amylovora
title_full Molecular characterization of Tunisian strains of Erwinia amylovora
title_fullStr Molecular characterization of Tunisian strains of Erwinia amylovora
title_full_unstemmed Molecular characterization of Tunisian strains of Erwinia amylovora
title_sort Molecular characterization of Tunisian strains of Erwinia amylovora
dc.creator.none.fl_str_mv Dardouri, Sana
Chehimi, Sonia
Murillo Martínez, Jesús
Hajlaoui, Mohamed Rabeh
author Dardouri, Sana
author_facet Dardouri, Sana
Chehimi, Sonia
Murillo Martínez, Jesús
Hajlaoui, Mohamed Rabeh
author_role author
author2 Chehimi, Sonia
Murillo Martínez, Jesús
Hajlaoui, Mohamed Rabeh
author2_role author
author
author
dc.contributor.none.fl_str_mv Producción Agraria
Nekazaritza Ekoizpena
dc.subject.none.fl_str_mv Fire blight
Erwinia amylovora
PCR
CRIS- PR
VNTR primers
Fingerprinting
Epidemiology
topic Fire blight
Erwinia amylovora
PCR
CRIS- PR
VNTR primers
Fingerprinting
Epidemiology
description The present study focused on the molecular characterization of a collection of Erwinia amylovora isolates recovered from different outbreaks in Tunisia between 2012 and 2014. Analysis of 54 isolates, including the reference type strain CFBP 1430, revealed that all Tunisian isolates produced the expected amplicons with diverse primer pairs routinely used for molecular diagnostics of E. amylovora. We also evaluated the genetic variability of these isolates by PCR fingerprinting, using specific primers for clustered regularly interspaced short palindromic repeats (CRISPRs) and for variable number of tandem repeats (VNTR) sequences. For the first method, our results revealed that all the primers used, except those for CRISPR3, which produced an identical amplicon for all isolates, showed some variability among Tunisian isolates. For the second method, forty-nine isolates showed the same fingerprint patterns as the reference type strain CFBP 1430 with all the primers used, whereas four of the isolates showed very divergent patterns. These results suggest that there has been a main introduction of European-type isolates in Tunisia, and possibly a few mutations or other independent introductions of the pathogen. Additionally, these results indicate that PCR fingerprinting using VNTR markers is a most useful tool for discriminating among E. amylovora strains and for their identification in epidemiological studies.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/25081
url https://hdl.handle.net/2454/25081
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0)
https://creativecommons.org/licenses/by-sa/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA 4.0)
https://creativecommons.org/licenses/by-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Italian Phytopathological Society
publisher.none.fl_str_mv Italian Phytopathological Society
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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