Characterization of the Protective Cellular Immune Response in Pigs Immunized Intradermally with the Live Attenuated African Swine Fever Virus (ASFV) Lv17/WB/Rie1

24 Pág.

Detalles Bibliográficos
Autores: Pedrera, Miriam, Soler, Alejandro, Simón, Alicia, Casado, Nadia, Pérez, Covadonga, García-Casado, M.A., Fernández-Pacheco, Paloma, Sánchez-Cordón, Pedro J., Arias, Marisa, Gallardo, Carmina
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/353615
Acceso en línea:http://hdl.handle.net/10261/353615
Access Level:acceso abierto
Palabra clave:African swine fever
Live-attenuated virus
Vaccine
Protective cellular response
T-cell
Cytokines
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spelling Characterization of the Protective Cellular Immune Response in Pigs Immunized Intradermally with the Live Attenuated African Swine Fever Virus (ASFV) Lv17/WB/Rie1Pedrera, MiriamSoler, AlejandroSimón, AliciaCasado, NadiaPérez, CovadongaGarcía-Casado, M.A.Fernández-Pacheco, PalomaSánchez-Cordón, Pedro J.Arias, MarisaGallardo, CarminaAfrican swine feverLive-attenuated virusVaccineProtective cellular responseT-cellCytokines24 Pág.Candidate vaccines against African swine fever virus (ASFV) based on naturally attenuated or genetically modified viruses have the potential to generate protective immune responses, although there is no consensus on what defines a protective immune response against ASFV. Studies, especially in sensible host species, focused on unravelling protective mechanisms will contribute to the development of safer and more effective vaccines. The present study provides a detailed analysis of phenotypic and functional data on cellular responses induced by intradermal immunization and subsequent boosting of domestic pigs with the naturally attenuated field strain Lv17/WB/Rie1, as well as the mechanisms underlying protection against intramuscular challenge with the virulent genotype II Armenia/07 strain. The transient mild to moderate increase of IL-8 and IL-10 in serum observed after immunization might be directly correlated with survival. Protection was also associated with a robust ASFV-specific polyfunctional memory T cell response, where CD4CD8 and CD8 T cells were identified as the main cellular sources of virus-specific IFNγ and TNFα. In parallel to cytokine response, these T-cell subsets also showed specific cytotoxic activity as evidenced by the increased expression of the CD107a degranulation marker. Along with virus specific multifunctional CD4CD8 and CD8 T responses, the increased levels of antigen experienced cytotoxic CD4 T cells observed after challenge in immunized pigs might also contribute to control virulent infection by killing mechanisms targeting infected antigen-presenting cells. Future studies should elucidate whether the memory T-cell responses evidenced in the present study persist and provide long-term protection against further ASFV infections.The research project “VACDIVA” was funded by the European Union’s Horizon 2020 Research and Innovation Program under the grant agreement no. 862874.Peer reviewedMultidisciplinary Digital Publishing InstituteEuropean CommissionPedrera, Miriam [0000-0003-4868-6630]Simón, Alicia [0009-0000-4743-7363]Casado, Nadia [0000-0002-8373-4928]Pérez, Covadonga [0000-0002-5295-6480]Sánchez-Cordón, P. J. [0000-0002-2407-6259]Gallardo, Carmina [0000-0003-3293-306X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/353615reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/Centro de Investigación en Sanidad Animal (CISA)https://doi.org/10.20944/preprints202403.0438.v1Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3536152026-05-22T06:33:51Z
dc.title.none.fl_str_mv Characterization of the Protective Cellular Immune Response in Pigs Immunized Intradermally with the Live Attenuated African Swine Fever Virus (ASFV) Lv17/WB/Rie1
title Characterization of the Protective Cellular Immune Response in Pigs Immunized Intradermally with the Live Attenuated African Swine Fever Virus (ASFV) Lv17/WB/Rie1
spellingShingle Characterization of the Protective Cellular Immune Response in Pigs Immunized Intradermally with the Live Attenuated African Swine Fever Virus (ASFV) Lv17/WB/Rie1
Pedrera, Miriam
African swine fever
Live-attenuated virus
Vaccine
Protective cellular response
T-cell
Cytokines
title_short Characterization of the Protective Cellular Immune Response in Pigs Immunized Intradermally with the Live Attenuated African Swine Fever Virus (ASFV) Lv17/WB/Rie1
title_full Characterization of the Protective Cellular Immune Response in Pigs Immunized Intradermally with the Live Attenuated African Swine Fever Virus (ASFV) Lv17/WB/Rie1
title_fullStr Characterization of the Protective Cellular Immune Response in Pigs Immunized Intradermally with the Live Attenuated African Swine Fever Virus (ASFV) Lv17/WB/Rie1
title_full_unstemmed Characterization of the Protective Cellular Immune Response in Pigs Immunized Intradermally with the Live Attenuated African Swine Fever Virus (ASFV) Lv17/WB/Rie1
title_sort Characterization of the Protective Cellular Immune Response in Pigs Immunized Intradermally with the Live Attenuated African Swine Fever Virus (ASFV) Lv17/WB/Rie1
dc.creator.none.fl_str_mv Pedrera, Miriam
Soler, Alejandro
Simón, Alicia
Casado, Nadia
Pérez, Covadonga
García-Casado, M.A.
Fernández-Pacheco, Paloma
Sánchez-Cordón, Pedro J.
Arias, Marisa
Gallardo, Carmina
author Pedrera, Miriam
author_facet Pedrera, Miriam
Soler, Alejandro
Simón, Alicia
Casado, Nadia
Pérez, Covadonga
García-Casado, M.A.
Fernández-Pacheco, Paloma
Sánchez-Cordón, Pedro J.
Arias, Marisa
Gallardo, Carmina
author_role author
author2 Soler, Alejandro
Simón, Alicia
Casado, Nadia
Pérez, Covadonga
García-Casado, M.A.
Fernández-Pacheco, Paloma
Sánchez-Cordón, Pedro J.
Arias, Marisa
Gallardo, Carmina
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Pedrera, Miriam [0000-0003-4868-6630]
Simón, Alicia [0009-0000-4743-7363]
Casado, Nadia [0000-0002-8373-4928]
Pérez, Covadonga [0000-0002-5295-6480]
Sánchez-Cordón, P. J. [0000-0002-2407-6259]
Gallardo, Carmina [0000-0003-3293-306X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv African swine fever
Live-attenuated virus
Vaccine
Protective cellular response
T-cell
Cytokines
topic African swine fever
Live-attenuated virus
Vaccine
Protective cellular response
T-cell
Cytokines
description 24 Pág.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/353615
url http://hdl.handle.net/10261/353615
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/
Centro de Investigación en Sanidad Animal (CISA)
https://doi.org/10.20944/preprints202403.0438.v1
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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