Molecular and Serological Study of Rickettsial Infection in Humans, and in Wild and Farm Animals, in the Province of Burgos, Spain

Limited information is available on the presence of rickettsial infection in humans and animal reservoirs in Spain. Exposure to spotted fever group rickettsia in healthy humans and in farm and wild animals in the Province of Burgos, Spain, was examined by serological methods. Rickettsial DNA was als...

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Detalhes bibliográficos
Autores: Lledó García, María Lourdes|||0000-0001-5387-6792, Domínguez Peñafiel, Fermín Gerardo, Giménez Pardo, Consuelo|||0000-0002-8206-1952, Gegúndez Cámara, María Isabel|||0000-0003-4986-1605, González, Rosario, Saz Pérez, José Vicente
Formato: artículo
Fecha de publicación:2014
País:España
Recursos:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/61223
Acesso em linha:http://hdl.handle.net/10017/61223
https://dx.doi.org/10.1089/vbz.2013.1513
Access Level:acceso abierto
Palavra-chave:Epidemiology
Rickettsia
Vector-borne
Reservoir host
Medicina
Medicine
Descrição
Resumo:Limited information is available on the presence of rickettsial infection in humans and animal reservoirs in Spain. Exposure to spotted fever group rickettsia in healthy humans and in farm and wild animals in the Province of Burgos, Spain, was examined by serological methods. Rickettsial DNA was also sought by PCR in animal samples. Of 102 human serum samples examined by indirect immunofluorescence assays (IFA), 5.88% were positive for antibodies against Rickettsia conorii (titers 1/128-1/512). Significant differences were detected in human seroprevalence with respect to age. In further IFAs, 102 out of 375 (27.2%) serum samples from the wild animals reacted with R. conorii antigens (titers 1/64-1/1024); 32 out of 281 (11.38%) samples from farm animals were also positive for R. conorii (titers 1/64-1/2048). The prevalence detected among total wild animals was significantly higher than among total farm animals. No rickettsial DNA was found by PCR in any farm or wild animal sample.