Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize

Coumarate 3-hydroxylase (C3H) catalyzes a key step of the synthesis of the two main lignin subunits, guaiacyl (G) and syringyl (S) in dicotyledonous species. As no functional data are available in regards to this enzyme in monocotyledonous species, we generated C3H1 knock-down maize plants. The resu...

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Autores: Fornalé, Silvia, Rencoret, Jorge, García-Calvo, Laura, Capellades, Montserrat, Encina, Antonio, Santiago Carabelos, Rogelio, Rigau, Joan, Gutiérrez Suárez, Ana, Río Andrade, José Carlos del, Caparrós Ruiz, David
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/116388
Acceso en línea:http://hdl.handle.net/10261/116388
Access Level:acceso abierto
Palabra clave:Degradability
Flavonoids
Lignin
Maize
Cell wall polysaccharides
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spelling Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maizeFornalé, SilviaRencoret, JorgeGarcía-Calvo, LauraCapellades, MontserratEncina, AntonioSantiago Carabelos, RogelioRigau, JoanGutiérrez Suárez, AnaRío Andrade, José Carlos delCaparrós Ruiz, DavidDegradabilityFlavonoidsLigninMaizeCell wall polysaccharidesCoumarate 3-hydroxylase (C3H) catalyzes a key step of the synthesis of the two main lignin subunits, guaiacyl (G) and syringyl (S) in dicotyledonous species. As no functional data are available in regards to this enzyme in monocotyledonous species, we generated C3H1 knock-down maize plants. The results obtained indicate that C3H1 participates in lignin biosynthesis as its down-regulation redirects the phenylpropanoid flux: as a result, increased amounts of p-hydroxyphenyl (H) units, lignin-associated ferulates and the flavone tricin were detected in transgenic stems cell walls. Altogether, these changes make stem cell walls more degradable in the most C3H1-repressed plants, despite their unaltered polysaccharide content. The increase in H monomers is moderate compared to C3H deficient Arabidopsis and alfalfa plants. This could be due to the existence of a second maize C3H protein (C3H2) that can compensate the reduced levels of C3H1 in these C3H1-RNAi maize plants. The reduced expression of C3H1 alters the macroscopic phenotype of the plants, whose growth is inhibited proportionally to the extent of C3H1 repression. Finally, the down-regulation of C3H1 also increases the synthesis of flavonoids, leading to the accumulation of anthocyanins in transgenic leaves.Funding: This work was supported by the Spanish “Ministerio de Economía y Competitividad” [AGL2011-30545-C02-01 to D.C.-R., AGL2011-30545-C02-02 to A.E., and AGL2011-25379 (co-financed by FEDER funds) to J.C.R.] and received financial support from the CONSOLIDER-INGENIO program [CSD2007-00036] from the Spanish Ministerio de Ciencia e Innovación. This work was carried out within the framework of the “Xarxa de Referència de Biotecnologia” (XarBa) from the Autonomous Government of Catalonia. R.S. was financed by the postdoctoral contracts “Isidro Parga Pondal” supported by the Autonomous Government of Galicia and the European Social Fund and “Ramón y Cajal”, supported by the Spanish Ministry of Economy and Competitiveness and the University of Vigo. J.R. thanks CSIC for a JAE-DOC contract of the program “Junta para la Ampliación de Estudios” co-financed by the European Social Fund.Peer ReviewedElsevier2015201520152015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/116388reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.plantsci.2015.04.007info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1163882026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize
title Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize
spellingShingle Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize
Fornalé, Silvia
Degradability
Flavonoids
Lignin
Maize
Cell wall polysaccharides
title_short Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize
title_full Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize
title_fullStr Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize
title_full_unstemmed Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize
title_sort Cell wall modifications triggered by the down-regulation of Coumarate 3-hydroxylase-1 in maize
dc.creator.none.fl_str_mv Fornalé, Silvia
Rencoret, Jorge
García-Calvo, Laura
Capellades, Montserrat
Encina, Antonio
Santiago Carabelos, Rogelio
Rigau, Joan
Gutiérrez Suárez, Ana
Río Andrade, José Carlos del
Caparrós Ruiz, David
author Fornalé, Silvia
author_facet Fornalé, Silvia
Rencoret, Jorge
García-Calvo, Laura
Capellades, Montserrat
Encina, Antonio
Santiago Carabelos, Rogelio
Rigau, Joan
Gutiérrez Suárez, Ana
Río Andrade, José Carlos del
Caparrós Ruiz, David
author_role author
author2 Rencoret, Jorge
García-Calvo, Laura
Capellades, Montserrat
Encina, Antonio
Santiago Carabelos, Rogelio
Rigau, Joan
Gutiérrez Suárez, Ana
Río Andrade, José Carlos del
Caparrós Ruiz, David
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Degradability
Flavonoids
Lignin
Maize
Cell wall polysaccharides
topic Degradability
Flavonoids
Lignin
Maize
Cell wall polysaccharides
description Coumarate 3-hydroxylase (C3H) catalyzes a key step of the synthesis of the two main lignin subunits, guaiacyl (G) and syringyl (S) in dicotyledonous species. As no functional data are available in regards to this enzyme in monocotyledonous species, we generated C3H1 knock-down maize plants. The results obtained indicate that C3H1 participates in lignin biosynthesis as its down-regulation redirects the phenylpropanoid flux: as a result, increased amounts of p-hydroxyphenyl (H) units, lignin-associated ferulates and the flavone tricin were detected in transgenic stems cell walls. Altogether, these changes make stem cell walls more degradable in the most C3H1-repressed plants, despite their unaltered polysaccharide content. The increase in H monomers is moderate compared to C3H deficient Arabidopsis and alfalfa plants. This could be due to the existence of a second maize C3H protein (C3H2) that can compensate the reduced levels of C3H1 in these C3H1-RNAi maize plants. The reduced expression of C3H1 alters the macroscopic phenotype of the plants, whose growth is inhibited proportionally to the extent of C3H1 repression. Finally, the down-regulation of C3H1 also increases the synthesis of flavonoids, leading to the accumulation of anthocyanins in transgenic leaves.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/116388
url http://hdl.handle.net/10261/116388
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.plantsci.2015.04.007
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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