Pancreatic changes in somatostatin content and receptor-effector system after intrapancreatic injection of 5,7-dihydroxytryptamine

To date, it is unknown whether intrapancreatic serotonergic nerves can influence pancreatic somatostatin (SS) content and the SS receptor/effector system in the exocrine pancreas. In this study, the intrapancreatic serotonergic nerves were chemically ablated by injecting a specific serotonin (5-HT)...

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Detalles Bibliográficos
Autores: Muñoz Acedo, Gema, Álvaro Alonso, Itziar, Arilla Ferreiro, Eduardo
Tipo de recurso: artículo
Fecha de publicación:1995
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/3216
Acceso en línea:http://hdl.handle.net/10017/3216
https://dx.doi.org/10.1677/joe.0.1450227
Access Level:acceso abierto
Palabra clave:Acinar cells
Guinea pig
Adenylate cyclase
Amylase secretion
Exocrine pancreas
5-HT1P receptors
Rat brain
Binding
Identification
Forskolin
Bioquímica
Ciencia
Biochemistry
Science
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spelling Pancreatic changes in somatostatin content and receptor-effector system after intrapancreatic injection of 5,7-dihydroxytryptamineMuñoz Acedo, GemaÁlvaro Alonso, ItziarArilla Ferreiro, EduardoAcinar cellsGuinea pigAdenylate cyclaseAmylase secretionExocrine pancreas5-HT1P receptorsRat brainBindingIdentificationForskolinBioquímicaCienciaBiochemistryScienceTo date, it is unknown whether intrapancreatic serotonergic nerves can influence pancreatic somatostatin (SS) content and the SS receptor/effector system in the exocrine pancreas. In this study, the intrapancreatic serotonergic nerves were chemically ablated by injecting a specific serotonin (5-HT) neurotoxin, 5,7-dihydroxytryptamine (5,7-DHT), into the substance of the gland. Three days after the injection, the 5-HT-like immunoreactive levels in the pancreas were reduced by more than 85% whereas somatostatin-like immunoreactive levels had increased (86%). The number of SS receptors in the pancreatic acinar cell membranes of the 5,7-DHT-treated rats was also increased (72%). No significant differences were seen in basal or forskolin-stimulated adenylate cyclase (AC) enzyme activities in the control and the 5,7-DHT-treated groups. In spite of the increase in the number of SS receptors in the pancreatic acinar cell membranes of 5,7-DHT-treated rats, SS caused a significantly lower inhibition of AC activity in these membranes. This finding is related to the observed decrease of a 41 kD pertussis toxin-sensitive substrate, presumably the αi subunit of the guanine nucleotide inhibitory protein, in pancreatic acinar cell membranes 3 days after intrapancreatic 5,7-DHT administration when compared with the corresponding controls. The functions of pancreatic serotonergic nerves seem to be associated with enteropancreatic communication. These data together with the present results suggest that pancreatic SS content and the SS receptor/effector system in the exocrine pancreas may be regulated by enteropancreatic serotonergic nerve fibers and may participate in enteropancreatic reflexes.Dirección General de Investigación Científica y Técnica19951995-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/3216https://dx.doi.org/10.1677/joe.0.1450227reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/32162026-06-18T11:13:07Z
dc.title.none.fl_str_mv Pancreatic changes in somatostatin content and receptor-effector system after intrapancreatic injection of 5,7-dihydroxytryptamine
title Pancreatic changes in somatostatin content and receptor-effector system after intrapancreatic injection of 5,7-dihydroxytryptamine
spellingShingle Pancreatic changes in somatostatin content and receptor-effector system after intrapancreatic injection of 5,7-dihydroxytryptamine
Muñoz Acedo, Gema
Acinar cells
Guinea pig
Adenylate cyclase
Amylase secretion
Exocrine pancreas
5-HT1P receptors
Rat brain
Binding
Identification
Forskolin
Bioquímica
Ciencia
Biochemistry
Science
title_short Pancreatic changes in somatostatin content and receptor-effector system after intrapancreatic injection of 5,7-dihydroxytryptamine
title_full Pancreatic changes in somatostatin content and receptor-effector system after intrapancreatic injection of 5,7-dihydroxytryptamine
title_fullStr Pancreatic changes in somatostatin content and receptor-effector system after intrapancreatic injection of 5,7-dihydroxytryptamine
title_full_unstemmed Pancreatic changes in somatostatin content and receptor-effector system after intrapancreatic injection of 5,7-dihydroxytryptamine
title_sort Pancreatic changes in somatostatin content and receptor-effector system after intrapancreatic injection of 5,7-dihydroxytryptamine
dc.creator.none.fl_str_mv Muñoz Acedo, Gema
Álvaro Alonso, Itziar
Arilla Ferreiro, Eduardo
author Muñoz Acedo, Gema
author_facet Muñoz Acedo, Gema
Álvaro Alonso, Itziar
Arilla Ferreiro, Eduardo
author_role author
author2 Álvaro Alonso, Itziar
Arilla Ferreiro, Eduardo
author2_role author
author
dc.subject.none.fl_str_mv Acinar cells
Guinea pig
Adenylate cyclase
Amylase secretion
Exocrine pancreas
5-HT1P receptors
Rat brain
Binding
Identification
Forskolin
Bioquímica
Ciencia
Biochemistry
Science
topic Acinar cells
Guinea pig
Adenylate cyclase
Amylase secretion
Exocrine pancreas
5-HT1P receptors
Rat brain
Binding
Identification
Forskolin
Bioquímica
Ciencia
Biochemistry
Science
description To date, it is unknown whether intrapancreatic serotonergic nerves can influence pancreatic somatostatin (SS) content and the SS receptor/effector system in the exocrine pancreas. In this study, the intrapancreatic serotonergic nerves were chemically ablated by injecting a specific serotonin (5-HT) neurotoxin, 5,7-dihydroxytryptamine (5,7-DHT), into the substance of the gland. Three days after the injection, the 5-HT-like immunoreactive levels in the pancreas were reduced by more than 85% whereas somatostatin-like immunoreactive levels had increased (86%). The number of SS receptors in the pancreatic acinar cell membranes of the 5,7-DHT-treated rats was also increased (72%). No significant differences were seen in basal or forskolin-stimulated adenylate cyclase (AC) enzyme activities in the control and the 5,7-DHT-treated groups. In spite of the increase in the number of SS receptors in the pancreatic acinar cell membranes of 5,7-DHT-treated rats, SS caused a significantly lower inhibition of AC activity in these membranes. This finding is related to the observed decrease of a 41 kD pertussis toxin-sensitive substrate, presumably the αi subunit of the guanine nucleotide inhibitory protein, in pancreatic acinar cell membranes 3 days after intrapancreatic 5,7-DHT administration when compared with the corresponding controls. The functions of pancreatic serotonergic nerves seem to be associated with enteropancreatic communication. These data together with the present results suggest that pancreatic SS content and the SS receptor/effector system in the exocrine pancreas may be regulated by enteropancreatic serotonergic nerve fibers and may participate in enteropancreatic reflexes.
publishDate 1995
dc.date.none.fl_str_mv 1995
1995-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/3216
https://dx.doi.org/10.1677/joe.0.1450227
url http://hdl.handle.net/10017/3216
https://dx.doi.org/10.1677/joe.0.1450227
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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