Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cells

19 p.-6 fig.

Detalhes bibliográficos
Autores: Brieño-Enríquez, Miguel A., García-López, Jesús, Cárdenas, David B., Sylvain, Guibert, Elouan, Cleroux, Lukas, Děd, Hourcade, Juan D., Pěknicová, Jana, Weber, Michael, Del Mazo, Jesús
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/114176
Acesso em linha:http://hdl.handle.net/10261/114176
Access Level:acceso abierto
Palavra-chave:Venclozolin
MicroRNAs
Germ Cells
Transgenerational effects
Epigenetics
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spelling Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cellsVinclozolin Deregulates MicroRNAs in Germ CellsBrieño-Enríquez, Miguel A.García-López, JesúsCárdenas, David B.Sylvain, GuibertElouan, ClerouxLukas, DědHourcade, Juan D.Pěknicová, JanaWeber, MichaelDel Mazo, JesúsVenclozolinMicroRNAsGerm CellsTransgenerational effectsEpigenetics19 p.-6 fig.In mammals, germ cell differentiation is initiated in the Primordial Germ Cells (PGCs) during fetal development. Prenatal exposure to environmental toxicants such as endocrine disruptors may alter PGC differentiation, development of the male germline and induce transgenerational epigenetic disorders. The anti-androgenic compound vinclozolin represents a paradigmatic example of molecule causing transgenerational effects on germ cells. We performed prenatal exposure to vinclozolin in mice and analyzed the phenotypic and molecular changes in three successive generations. A reduction in the number of embryonic PGCs and increased rate of apoptotic cells along with decrease of fertility rate in adult males were observed in F1 to F3 generations. Blimp1 is a crucial regulator of PGC differentiation. We show that prenatal exposure to vinclozolin deregulates specific microRNAs in PGCs, such as miR-23b and miR-21, inducing disequilibrium in the Lin28/let-7/Blimp1 pathway in three successive generations of males. As determined by global maps of cytosine methylation, we found no evidence for prominent changes in DNA methylation in PGCs or mature sperm. Our data suggest that embryonic exposure to environmental endocrine disruptors induces transgenerational epigenetic deregulation of expression of microRNAs affecting key regulatory pathways of germ cells differentiation.We thank M. Quesada, O. Barcón and M. Moreno for caring for the animals. We also acknowledged the support from "EpiGeneSys European Network of Excellence".Peer reviewedPublic Library of ScienceConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201520152015info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/114176reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1371/journal.pone.0124296Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1141762026-05-22T06:33:51Z
dc.title.none.fl_str_mv Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cells
Vinclozolin Deregulates MicroRNAs in Germ Cells
title Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cells
spellingShingle Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cells
Brieño-Enríquez, Miguel A.
Venclozolin
MicroRNAs
Germ Cells
Transgenerational effects
Epigenetics
title_short Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cells
title_full Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cells
title_fullStr Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cells
title_full_unstemmed Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cells
title_sort Exposure to endocrine disruptor induces transgenerational epigenetic deregulation of microRNAs in primordial germ cells
dc.creator.none.fl_str_mv Brieño-Enríquez, Miguel A.
García-López, Jesús
Cárdenas, David B.
Sylvain, Guibert
Elouan, Cleroux
Lukas, Děd
Hourcade, Juan D.
Pěknicová, Jana
Weber, Michael
Del Mazo, Jesús
author Brieño-Enríquez, Miguel A.
author_facet Brieño-Enríquez, Miguel A.
García-López, Jesús
Cárdenas, David B.
Sylvain, Guibert
Elouan, Cleroux
Lukas, Děd
Hourcade, Juan D.
Pěknicová, Jana
Weber, Michael
Del Mazo, Jesús
author_role author
author2 García-López, Jesús
Cárdenas, David B.
Sylvain, Guibert
Elouan, Cleroux
Lukas, Děd
Hourcade, Juan D.
Pěknicová, Jana
Weber, Michael
Del Mazo, Jesús
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Venclozolin
MicroRNAs
Germ Cells
Transgenerational effects
Epigenetics
topic Venclozolin
MicroRNAs
Germ Cells
Transgenerational effects
Epigenetics
description 19 p.-6 fig.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/114176
url http://hdl.handle.net/10261/114176
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1371/journal.pone.0124296
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404142551695360
score 15,81155

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