A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymes

The human genome contains nearly 100 deubiquitinating enzymes (DUBs) responsible for removing ubiquitin moieties from a large variety of substrates. Which DUBs are responsible for targeting which substrates remain mostly unknown. Here we implement the bioUb approach to identify DUB substrates in a s...

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Detalhes bibliográficos
Autores: Ramirez, Juanma, Prieto, Gorka, Olazabal-Herrero, Anne, Borràs, Eva, Fernandez-Vigo, Elvira, Alduntzin, Unai, Osinalde, Nerea, Beaskoetxea, Javier, Lectez, Benoit, Aloria, Kerman, Rodríguez, José Antonio, Paradela, Alberto, Sabidó Aguadé, Eduard, 1981-, Muñoz, Javier, Corrales, Fernando, Arizmendi, Jesús M., Mayor, Ugo
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/49053
Acesso em linha:http://hdl.handle.net/10230/49053
http://dx.doi.org/10.3390/ijms22094851
Access Level:acceso abierto
Palavra-chave:DUBase
Deubiquitinating enzyme
Quantitative proteomics
Ubiquitination
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spelling A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymesRamirez, JuanmaPrieto, GorkaOlazabal-Herrero, AnneBorràs, EvaFernandez-Vigo, ElviraAlduntzin, UnaiOsinalde, NereaBeaskoetxea, JavierLectez, BenoitAloria, KermanRodríguez, José AntonioParadela, AlbertoSabidó Aguadé, Eduard, 1981-Muñoz, JavierCorrales, FernandoArizmendi, Jesús M.Mayor, UgoDUBaseDeubiquitinating enzymeQuantitative proteomicsUbiquitinationThe human genome contains nearly 100 deubiquitinating enzymes (DUBs) responsible for removing ubiquitin moieties from a large variety of substrates. Which DUBs are responsible for targeting which substrates remain mostly unknown. Here we implement the bioUb approach to identify DUB substrates in a systematic manner, combining gene silencing and proteomics analyses. Silencing of individual DUB enzymes is used to reduce their ubiquitin deconjugating activity, leading to an increase of the ubiquitination of their substrates, which can then be isolated and identified. We report here quantitative proteomic data of the putative substrates of 5 human DUBs. Furthermore, we have built a novel interactive database of DUB substrates to provide easy access to our data and collect DUB proteome data from other groups as a reference resource in the DUB substrates research field.Authors of this paper are members of Proteored, PRB3 and are supported by grant PT17/0019 of the PE I+D+i 2013-2016, funded by Instituto de Salud Carlos III (ISCIII) and European Regional Development Fund (ERDF). This research was also funded by the Spanish Ministry of Economy, grant number SAF2016-76898-P and the University of the Basque Country (UPV/EHU), grant number US19/05. J.R was supported with a postdoctoral research fellowship from the UPV/EHU.MDPI202120212021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/49053http://dx.doi.org/10.3390/ijms22094851reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésInt J Mol Sci. 2021;22(9):4851info:eu-repo/grantAgreement/ES/1PE/SAF2016-76898-P© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/490532026-05-29T05:05:01Z
dc.title.none.fl_str_mv A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymes
title A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymes
spellingShingle A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymes
Ramirez, Juanma
DUBase
Deubiquitinating enzyme
Quantitative proteomics
Ubiquitination
title_short A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymes
title_full A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymes
title_fullStr A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymes
title_full_unstemmed A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymes
title_sort A proteomic approach for systematic mapping of substrates of human deubiquitinating enzymes
dc.creator.none.fl_str_mv Ramirez, Juanma
Prieto, Gorka
Olazabal-Herrero, Anne
Borràs, Eva
Fernandez-Vigo, Elvira
Alduntzin, Unai
Osinalde, Nerea
Beaskoetxea, Javier
Lectez, Benoit
Aloria, Kerman
Rodríguez, José Antonio
Paradela, Alberto
Sabidó Aguadé, Eduard, 1981-
Muñoz, Javier
Corrales, Fernando
Arizmendi, Jesús M.
Mayor, Ugo
author Ramirez, Juanma
author_facet Ramirez, Juanma
Prieto, Gorka
Olazabal-Herrero, Anne
Borràs, Eva
Fernandez-Vigo, Elvira
Alduntzin, Unai
Osinalde, Nerea
Beaskoetxea, Javier
Lectez, Benoit
Aloria, Kerman
Rodríguez, José Antonio
Paradela, Alberto
Sabidó Aguadé, Eduard, 1981-
Muñoz, Javier
Corrales, Fernando
Arizmendi, Jesús M.
Mayor, Ugo
author_role author
author2 Prieto, Gorka
Olazabal-Herrero, Anne
Borràs, Eva
Fernandez-Vigo, Elvira
Alduntzin, Unai
Osinalde, Nerea
Beaskoetxea, Javier
Lectez, Benoit
Aloria, Kerman
Rodríguez, José Antonio
Paradela, Alberto
Sabidó Aguadé, Eduard, 1981-
Muñoz, Javier
Corrales, Fernando
Arizmendi, Jesús M.
Mayor, Ugo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DUBase
Deubiquitinating enzyme
Quantitative proteomics
Ubiquitination
topic DUBase
Deubiquitinating enzyme
Quantitative proteomics
Ubiquitination
description The human genome contains nearly 100 deubiquitinating enzymes (DUBs) responsible for removing ubiquitin moieties from a large variety of substrates. Which DUBs are responsible for targeting which substrates remain mostly unknown. Here we implement the bioUb approach to identify DUB substrates in a systematic manner, combining gene silencing and proteomics analyses. Silencing of individual DUB enzymes is used to reduce their ubiquitin deconjugating activity, leading to an increase of the ubiquitination of their substrates, which can then be isolated and identified. We report here quantitative proteomic data of the putative substrates of 5 human DUBs. Furthermore, we have built a novel interactive database of DUB substrates to provide easy access to our data and collect DUB proteome data from other groups as a reference resource in the DUB substrates research field.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/49053
http://dx.doi.org/10.3390/ijms22094851
url http://hdl.handle.net/10230/49053
http://dx.doi.org/10.3390/ijms22094851
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Int J Mol Sci. 2021;22(9):4851
info:eu-repo/grantAgreement/ES/1PE/SAF2016-76898-P
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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