A label-free optical system with a nanohole array biosensor for discriminating live single cancer cells from normal cells
Developing a simple, fast, and label-free method for discrimination between live cancer cells and normal cells in biological samples still remains a challenge. Here, a system is described that fulfills these features to analyze individual living cells. The system consists of a gold nanohole array bi...
| Autores: | , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universidad de Cantabria (UC) |
| Repositorio: | UCrea Repositorio Abierto de la Universidad de Cantabria |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.unican.es:10902/24587 |
| Acceso en línea: | http://hdl.handle.net/10902/24587 |
| Access Level: | acceso abierto |
| Palabra clave: | Actin cortex Cancer cell Extraordinary optical transmission Plasmonics |
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oai:repositorio.unican.es:10902/24587 |
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A label-free optical system with a nanohole array biosensor for discriminating live single cancer cells from normal cellsFranco Pérez, Alfredo|||0000-0003-1803-6258Vidal Sánchez, VerónicaGómez Ruiz, MarcosGutiérrez Saiz, María OlgaMartino González, MaríaGonzález Fernández, Francisco|||0000-0002-2944-4903Moreno Gracia, Fernando|||0000-0003-3171-7285Fernández Luna, José LuisActin cortexCancer cellExtraordinary optical transmissionPlasmonicsDeveloping a simple, fast, and label-free method for discrimination between live cancer cells and normal cells in biological samples still remains a challenge. Here, a system is described that fulfills these features to analyze individual living cells. The system consists of a gold nanohole array biosensor plus a microscope optical design to isolate the spectral response of a single cell. It is demonstrated that differences in the spectral behavior between tumor (colorectal cancer cell lines and primary cells from colorectal cancer tissue) and non-tumor cells (peripheral blood mononuclear cells, skin fibroblasts and colon epithelial cells) are influenced by the actin cortex, which lies within the short penetration depth of the surface plasmon electromagnetic field. The efficacy of this system was assessed by the analysis of about one thousand single cells showing the highest discrimination capacity between normal colon epithelial cells and colorectal cancer cells from surgical specimens, with values of sensitivity and specificity ranging 80-100% and 87-100%, respectively. It is also demonstrated that cell discrimination capacity of the system is highly reduced by disrupting the formation of actin cortex. This plasmonic system may find wide applications in biomedicine and to study key cellular processes that involve the actin cortex, including proliferation, differentiation, and migration.This work was supported by the Instituto de Salud Carlos III through grant DTS18/00141, co-funded by European Regional Development Fund/ European Social Fund “A way to make Europe/Investing in your future” and Instituto de Investigación Valdecilla (IDIVAL) (APG/03).De GruyterUniversidad de Cantabria20222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articlehttp://hdl.handle.net/10902/24587Nanophotonics, 2021, 11(2), 315 - 328reponame:UCrea Repositorio Abierto de la Universidad de Cantabriainstname:Universidad de Cantabria (UC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nd/4.0/info:eu-repo/semantics/openAccessoai:repositorio.unican.es:10902/245872026-06-02T12:39:31Z |
| dc.title.none.fl_str_mv |
A label-free optical system with a nanohole array biosensor for discriminating live single cancer cells from normal cells |
| title |
A label-free optical system with a nanohole array biosensor for discriminating live single cancer cells from normal cells |
| spellingShingle |
A label-free optical system with a nanohole array biosensor for discriminating live single cancer cells from normal cells Franco Pérez, Alfredo|||0000-0003-1803-6258 Actin cortex Cancer cell Extraordinary optical transmission Plasmonics |
| title_short |
A label-free optical system with a nanohole array biosensor for discriminating live single cancer cells from normal cells |
| title_full |
A label-free optical system with a nanohole array biosensor for discriminating live single cancer cells from normal cells |
| title_fullStr |
A label-free optical system with a nanohole array biosensor for discriminating live single cancer cells from normal cells |
| title_full_unstemmed |
A label-free optical system with a nanohole array biosensor for discriminating live single cancer cells from normal cells |
| title_sort |
A label-free optical system with a nanohole array biosensor for discriminating live single cancer cells from normal cells |
| dc.creator.none.fl_str_mv |
Franco Pérez, Alfredo|||0000-0003-1803-6258 Vidal Sánchez, Verónica Gómez Ruiz, Marcos Gutiérrez Saiz, María Olga Martino González, María González Fernández, Francisco|||0000-0002-2944-4903 Moreno Gracia, Fernando|||0000-0003-3171-7285 Fernández Luna, José Luis |
| author |
Franco Pérez, Alfredo|||0000-0003-1803-6258 |
| author_facet |
Franco Pérez, Alfredo|||0000-0003-1803-6258 Vidal Sánchez, Verónica Gómez Ruiz, Marcos Gutiérrez Saiz, María Olga Martino González, María González Fernández, Francisco|||0000-0002-2944-4903 Moreno Gracia, Fernando|||0000-0003-3171-7285 Fernández Luna, José Luis |
| author_role |
author |
| author2 |
Vidal Sánchez, Verónica Gómez Ruiz, Marcos Gutiérrez Saiz, María Olga Martino González, María González Fernández, Francisco|||0000-0002-2944-4903 Moreno Gracia, Fernando|||0000-0003-3171-7285 Fernández Luna, José Luis |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidad de Cantabria |
| dc.subject.none.fl_str_mv |
Actin cortex Cancer cell Extraordinary optical transmission Plasmonics |
| topic |
Actin cortex Cancer cell Extraordinary optical transmission Plasmonics |
| description |
Developing a simple, fast, and label-free method for discrimination between live cancer cells and normal cells in biological samples still remains a challenge. Here, a system is described that fulfills these features to analyze individual living cells. The system consists of a gold nanohole array biosensor plus a microscope optical design to isolate the spectral response of a single cell. It is demonstrated that differences in the spectral behavior between tumor (colorectal cancer cell lines and primary cells from colorectal cancer tissue) and non-tumor cells (peripheral blood mononuclear cells, skin fibroblasts and colon epithelial cells) are influenced by the actin cortex, which lies within the short penetration depth of the surface plasmon electromagnetic field. The efficacy of this system was assessed by the analysis of about one thousand single cells showing the highest discrimination capacity between normal colon epithelial cells and colorectal cancer cells from surgical specimens, with values of sensitivity and specificity ranging 80-100% and 87-100%, respectively. It is also demonstrated that cell discrimination capacity of the system is highly reduced by disrupting the formation of actin cortex. This plasmonic system may find wide applications in biomedicine and to study key cellular processes that involve the actin cortex, including proliferation, differentiation, and migration. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022-01-01 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 NA http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10902/24587 |
| url |
http://hdl.handle.net/10902/24587 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nd/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
De Gruyter |
| publisher.none.fl_str_mv |
De Gruyter |
| dc.source.none.fl_str_mv |
Nanophotonics, 2021, 11(2), 315 - 328 reponame:UCrea Repositorio Abierto de la Universidad de Cantabria instname:Universidad de Cantabria (UC) |
| instname_str |
Universidad de Cantabria (UC) |
| reponame_str |
UCrea Repositorio Abierto de la Universidad de Cantabria |
| collection |
UCrea Repositorio Abierto de la Universidad de Cantabria |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
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1869404137100148736 |
| score |
15.301603 |