Epididymal sperm maturation in mouse species with differing levels of sperm competition

The data underlying this article will be shared on reasonable request to the corresponding authors.

Detalhes bibliográficos
Autores: Agudo-Ríos, Clara, Sánchez-Rodríguez, Ana, Idrovo, Ingrid I. D., Laborda-Gomariz, Juan Ángel, Soler, Ana J., Valverde, Anthony, Teves, Maria E., Roldán, Eduardo R. S.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/422598
Acesso em linha:http://hdl.handle.net/10261/422598
Access Level:acceso abierto
Palavra-chave:Epididymis
DNA fragmentation
Sperm
Protamination
Acrosome
Nucleus
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dc.title.none.fl_str_mv Epididymal sperm maturation in mouse species with differing levels of sperm competition
title Epididymal sperm maturation in mouse species with differing levels of sperm competition
spellingShingle Epididymal sperm maturation in mouse species with differing levels of sperm competition
Agudo-Ríos, Clara
Epididymis
DNA fragmentation
Sperm
Protamination
Acrosome
Nucleus
title_short Epididymal sperm maturation in mouse species with differing levels of sperm competition
title_full Epididymal sperm maturation in mouse species with differing levels of sperm competition
title_fullStr Epididymal sperm maturation in mouse species with differing levels of sperm competition
title_full_unstemmed Epididymal sperm maturation in mouse species with differing levels of sperm competition
title_sort Epididymal sperm maturation in mouse species with differing levels of sperm competition
dc.creator.none.fl_str_mv Agudo-Ríos, Clara
Sánchez-Rodríguez, Ana
Idrovo, Ingrid I. D.
Laborda-Gomariz, Juan Ángel
Soler, Ana J.
Valverde, Anthony
Teves, Maria E.
Roldán, Eduardo R. S.
author Agudo-Ríos, Clara
author_facet Agudo-Ríos, Clara
Sánchez-Rodríguez, Ana
Idrovo, Ingrid I. D.
Laborda-Gomariz, Juan Ángel
Soler, Ana J.
Valverde, Anthony
Teves, Maria E.
Roldán, Eduardo R. S.
author_role author
author2 Sánchez-Rodríguez, Ana
Idrovo, Ingrid I. D.
Laborda-Gomariz, Juan Ángel
Soler, Ana J.
Valverde, Anthony
Teves, Maria E.
Roldán, Eduardo R. S.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas (España)
National Institutes of Health (US)
European Commission
Ministerio de Ciencia e Innovación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Epididymis
DNA fragmentation
Sperm
Protamination
Acrosome
Nucleus
topic Epididymis
DNA fragmentation
Sperm
Protamination
Acrosome
Nucleus
description The data underlying this article will be shared on reasonable request to the corresponding authors.
publishDate 2025
dc.date.none.fl_str_mv 2025
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
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dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/422598
url http://hdl.handle.net/10261/422598
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108649GB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2024-2027/PID2024-160374NB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126988OB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PRE2020-095265
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1093/biolre/ioaf269
https://doi.org/10.1093/biolre/ioaf269
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dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Epididymal sperm maturation in mouse species with differing levels of sperm competitionAgudo-Ríos, ClaraSánchez-Rodríguez, AnaIdrovo, Ingrid I. D.Laborda-Gomariz, Juan ÁngelSoler, Ana J.Valverde, AnthonyTeves, Maria E.Roldán, Eduardo R. S.EpididymisDNA fragmentationSpermProtaminationAcrosomeNucleusThe data underlying this article will be shared on reasonable request to the corresponding authors.During epididymal transit, spermatozoa undergo crucial morphological, biochemical, and molecular modifications that enable the acquisition of motility, acrosomal remodeling, membrane reorganization, and chromatin stabilization, culminating in full maturation. This study investigated sperm motility, sperm head and acrosome remodeling, chromatin compaction (protamination), DNA integrity, and nuclear morphology in sperm collected from different epididymal regions (caput, corpus, cauda) and the vas deferens in three closely related mouse species exhibiting varying levels of sperm competition. Using microscopy, fluorescent staining (Hoechst 33342, chromomycin A3), sperm chromatin structure assay (SCSA), and nuclear morphology software, we characterized these parameters. Results demonstrated a gradual acquisition of total and progressive motility from the caput onwards and a shift toward a more compacted acrosome, especially between corpus and cauda. Minor species-specific variations in nuclear shape and dimensions were observed. Chromatin compaction and DNA integrity significantly increased, evidenced by decreased chromomycin A3 positive cells and reduced DNA fragmentation indices (tDFI and HDS). A correlation between protamine status and DNA fragmentation appeared when data from all species were pooled. M. musculus showed lower overall motility and a faster decline in loose acrosomes. M. spicilegus exhibited the most rapid sperm head compaction, whereas M. spretus had the highest sperm nuclear width and the lowest tDFI values. These findings reveal distinct changes in sperm maturation markers along the epididymis and suggest associations between these changes and sperm competition levels of these species. This enhances our understanding of sperm maturation mechanisms and may inform advances in reproductive technologies.This work was supported by the Spanish Ministry of Science and Innovation grants PID2019-108649GB-I00 and PID2024-160374NB-I00 and the Spanish Higher Council for Scientific Research grant 2023AEP112, both to E.R.S.R., PID2021-126988OB-100 to A.J.S. and the National Institutes of Health grant R03HD101762 to M.E.T. C.A.-R. was supported by a predoctoral studentship (FPI) from the Spanish Agencia Estatal de Investigación, cofunded by the European Social Fund (PRE2020–095265), A.S.-R. was funded by a “Juan de la Cierva” postdoctoral fellowship and J.A.L.-G. was funded by a “Personal Técnico de Apoyo” scholarship, all from the Ministry of Science and Innovation, co-funded by the European Social Fund.Peer reviewedOxford University PressAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas (España)National Institutes of Health (US)European CommissionMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/422598reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108649GB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2024-2027/PID2024-160374NB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126988OB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PRE2020-095265The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.1093/biolre/ioaf269https://doi.org/10.1093/biolre/ioaf269Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4225982026-05-22T06:33:51Z
score 15,811543

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