Pioneering use of human intestinal enteroids to prevent foodborne transmission of human norovirus

Background: Human norovirus (HuNoV) is a leading cause of foodborne viral gastroenteritis and a significant public health concern. Its high transmission rates and ability to persist in diverse environments contribute to substantial morbidity and economic burden. Traditional cell culture methodologie...

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Autor: Randazzo, Walter
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/375239
Acceso en línea:http://hdl.handle.net/10261/375239
https://api.elsevier.com/content/abstract/scopus_id/85212542912
Access Level:acceso abierto
Palabra clave:Cell culture
Enteric virus
Food safety
Infectivity
Viral pathogen
food safety
viruses
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spelling Pioneering use of human intestinal enteroids to prevent foodborne transmission of human norovirusRandazzo, WalterCell cultureEnteric virusFood safetyInfectivityViral pathogenfood safetyvirusesBackground: Human norovirus (HuNoV) is a leading cause of foodborne viral gastroenteritis and a significant public health concern. Its high transmission rates and ability to persist in diverse environments contribute to substantial morbidity and economic burden. Traditional cell culture methodologies have proven inadequate for HuNoV research due to the inability of the virus to replicate in conventional cell lines. However, the recent development of human intestinal enteroid (HIE) cell cultures, which support HuNoV replication, has advanced our understanding of this challenging virus. Scope and approach: This review provides an updated analysis of research on the applications of the HIE model to control HuNoV across various food systems. It examines the challenges associated with HuNoV replication, and explores the potential of HIE models to help mitigate foodborne transmission of HuNoV. Finally, the review discusses the limitations of current HIE methodologies and outlines promising avenues of future research to improve preventive and control strategies, ultimately reducing the risk of HuNoV exposure for consumers. Key findings and conclusions: The HIE system offers a physiologically relevant model for studying HuNoV infectivity, survival, and inactivation, aiding the development of effective strategies to prevent and control foodborne transmission. While limited for routine detection, integrating HIE studies with RNA detection, surrogate research, data modeling, and risk assessment is recommended to comprehensively evaluate and enhance the reliability of strategies for real-world food safety applications.This work was supported by the PID 2019-105509RJ-I00 and PID 2023-149211OB-C32 projects funded by MICIU/AEI/10.13039/501100011033 and ERDF/EU. The Accreditation as Center of Excellence Severo Ochoa CEX 2021-001189-S funded by MCIU/AEI/10.13039/501100011033 is also fully acknowledged.Peer reviewedElsevierMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)Randazzo, Walter [0000-0002-7433-149X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_dcae04bcPublisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/375239https://api.elsevier.com/content/abstract/scopus_id/85212542912reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105509RJ-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-149211OB-C32Trends in Food Science and Technologyhttps://doi.org/10.1016/j.tifs.2024.104843Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3752392026-05-22T06:33:51Z
dc.title.none.fl_str_mv Pioneering use of human intestinal enteroids to prevent foodborne transmission of human norovirus
title Pioneering use of human intestinal enteroids to prevent foodborne transmission of human norovirus
spellingShingle Pioneering use of human intestinal enteroids to prevent foodborne transmission of human norovirus
Randazzo, Walter
Cell culture
Enteric virus
Food safety
Infectivity
Viral pathogen
food safety
viruses
title_short Pioneering use of human intestinal enteroids to prevent foodborne transmission of human norovirus
title_full Pioneering use of human intestinal enteroids to prevent foodborne transmission of human norovirus
title_fullStr Pioneering use of human intestinal enteroids to prevent foodborne transmission of human norovirus
title_full_unstemmed Pioneering use of human intestinal enteroids to prevent foodborne transmission of human norovirus
title_sort Pioneering use of human intestinal enteroids to prevent foodborne transmission of human norovirus
dc.creator.none.fl_str_mv Randazzo, Walter
author Randazzo, Walter
author_facet Randazzo, Walter
author_role author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
Randazzo, Walter [0000-0002-7433-149X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cell culture
Enteric virus
Food safety
Infectivity
Viral pathogen
food safety
viruses
topic Cell culture
Enteric virus
Food safety
Infectivity
Viral pathogen
food safety
viruses
description Background: Human norovirus (HuNoV) is a leading cause of foodborne viral gastroenteritis and a significant public health concern. Its high transmission rates and ability to persist in diverse environments contribute to substantial morbidity and economic burden. Traditional cell culture methodologies have proven inadequate for HuNoV research due to the inability of the virus to replicate in conventional cell lines. However, the recent development of human intestinal enteroid (HIE) cell cultures, which support HuNoV replication, has advanced our understanding of this challenging virus. Scope and approach: This review provides an updated analysis of research on the applications of the HIE model to control HuNoV across various food systems. It examines the challenges associated with HuNoV replication, and explores the potential of HIE models to help mitigate foodborne transmission of HuNoV. Finally, the review discusses the limitations of current HIE methodologies and outlines promising avenues of future research to improve preventive and control strategies, ultimately reducing the risk of HuNoV exposure for consumers. Key findings and conclusions: The HIE system offers a physiologically relevant model for studying HuNoV infectivity, survival, and inactivation, aiding the development of effective strategies to prevent and control foodborne transmission. While limited for routine detection, integrating HIE studies with RNA detection, surrogate research, data modeling, and risk assessment is recommended to comprehensively evaluate and enhance the reliability of strategies for real-world food safety applications.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_dcae04bc
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/375239
https://api.elsevier.com/content/abstract/scopus_id/85212542912
url http://hdl.handle.net/10261/375239
https://api.elsevier.com/content/abstract/scopus_id/85212542912
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105509RJ-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-149211OB-C32
Trends in Food Science and Technology
https://doi.org/10.1016/j.tifs.2024.104843
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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