Functionalization of 3D printed polymeric bioresorbable stents with a dual cell-adhesive peptidic platform combining RGDS and YIGSR sequences

Biomimetic surface modification with cell-adhesive peptides is a promising approach to improve endothelialization of current bioresorbable stents (BRS). Among them, RGDS and YIGSR sequences have been reported to mediate adhesion and migration of endothelial cells (ECs) while preventing platelet acti...

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Autores: Chausse Calbet, Victor|||0000-0002-2644-6305, Mas Moruno, Carlos|||0000-0001-8337-0872, Martín Gómez, Helena|||0000-0001-8956-116X, Pino Curto, Marc, Díaz Ricart, Maribel, Escolar Albaladejo, Ginés, Ginebra Molins, Maria Pau|||0000-0002-4700-5621, Pegueroles Neyra, Marta|||0000-0002-7895-8337
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Politècnica de Catalunya (UPC)
Repositorio:UPCommons. Portal del coneixement obert de la UPC
Idioma:inglés
OAI Identifier:oai:upcommons.upc.edu:2117/397428
Acceso en línea:https://hdl.handle.net/2117/397428
https://dx.doi.org/10.1039/d3bm00458a
Access Level:acceso abierto
Palabra clave:Biomedical materials
Materials biomèdics
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
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spelling Functionalization of 3D printed polymeric bioresorbable stents with a dual cell-adhesive peptidic platform combining RGDS and YIGSR sequencesChausse Calbet, Victor|||0000-0002-2644-6305Mas Moruno, Carlos|||0000-0001-8337-0872Martín Gómez, Helena|||0000-0001-8956-116XPino Curto, MarcDíaz Ricart, MaribelEscolar Albaladejo, GinésGinebra Molins, Maria Pau|||0000-0002-4700-5621Pegueroles Neyra, Marta|||0000-0002-7895-8337Biomedical materialsMaterials biomèdicsÀrees temàtiques de la UPC::Enginyeria biomèdica::BiomaterialsBiomimetic surface modification with cell-adhesive peptides is a promising approach to improve endothelialization of current bioresorbable stents (BRS). Among them, RGDS and YIGSR sequences have been reported to mediate adhesion and migration of endothelial cells (ECs) while preventing platelet activation. This work presents the functionalization of novel 3D-printed poly-L-lactic acid (PLLA) and poly(L-lactic-co-e-caprolactone) (PLCL) BRS with linear RGDS and YIGSR sequences, as well as a dual platform (PF) containing both motifs within a single biomolecule. Functionalized surfaces were characterized in terms of static contact angle, biomolecule distribution under confocal fluorescence microscopy and peptide quantification via detachment from the surface, showing a biomolecule density in the range of 0.5 to 3.5 nmol cm-2. Biological evaluation comprised a cell adhesion test on functionalized films with ECs and a blood perfusion assay on functionalized stents to assess ECs response and device hemocompatibility, respectively. Cell adhesion assays evidenced significantly increased cell number and spreading onto functionalized films with respect to control samples. Regarding stents’ hemocompatibility, platelet adhesion onto PLCL stents was severely decreased with respect to PLLA. In addition, functionalization with RGDS, YIGSR and the PF rendered BRS stents displaying even further reduced platelet adhesion. In conclusion, the combination of intrinsically less prothrombogenic materials such as PLCL and its functionalization with EC-discriminating adhesive biomolecules paves the way for a new generation of BRS based on accelerated re-endothelialization approaches.Peer ReviewedRoyal Society of Chemistry (RSC)20232023-05-0820232023-11-30journal articlehttp://purl.org/coar/resource_type/c_6501AMhttp://purl.org/coar/version/c_ab4af688f83e57aainfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/2117/397428https://dx.doi.org/10.1039/d3bm00458areponame:UPCommons. Portal del coneixement obert de la UPCinstname:Universitat Politècnica de Catalunya (UPC)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:upcommons.upc.edu:2117/3974282026-05-27T15:37:01Z
dc.title.none.fl_str_mv Functionalization of 3D printed polymeric bioresorbable stents with a dual cell-adhesive peptidic platform combining RGDS and YIGSR sequences
title Functionalization of 3D printed polymeric bioresorbable stents with a dual cell-adhesive peptidic platform combining RGDS and YIGSR sequences
spellingShingle Functionalization of 3D printed polymeric bioresorbable stents with a dual cell-adhesive peptidic platform combining RGDS and YIGSR sequences
Chausse Calbet, Victor|||0000-0002-2644-6305
Biomedical materials
Materials biomèdics
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
title_short Functionalization of 3D printed polymeric bioresorbable stents with a dual cell-adhesive peptidic platform combining RGDS and YIGSR sequences
title_full Functionalization of 3D printed polymeric bioresorbable stents with a dual cell-adhesive peptidic platform combining RGDS and YIGSR sequences
title_fullStr Functionalization of 3D printed polymeric bioresorbable stents with a dual cell-adhesive peptidic platform combining RGDS and YIGSR sequences
title_full_unstemmed Functionalization of 3D printed polymeric bioresorbable stents with a dual cell-adhesive peptidic platform combining RGDS and YIGSR sequences
title_sort Functionalization of 3D printed polymeric bioresorbable stents with a dual cell-adhesive peptidic platform combining RGDS and YIGSR sequences
dc.creator.none.fl_str_mv Chausse Calbet, Victor|||0000-0002-2644-6305
Mas Moruno, Carlos|||0000-0001-8337-0872
Martín Gómez, Helena|||0000-0001-8956-116X
Pino Curto, Marc
Díaz Ricart, Maribel
Escolar Albaladejo, Ginés
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Pegueroles Neyra, Marta|||0000-0002-7895-8337
author Chausse Calbet, Victor|||0000-0002-2644-6305
author_facet Chausse Calbet, Victor|||0000-0002-2644-6305
Mas Moruno, Carlos|||0000-0001-8337-0872
Martín Gómez, Helena|||0000-0001-8956-116X
Pino Curto, Marc
Díaz Ricart, Maribel
Escolar Albaladejo, Ginés
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Pegueroles Neyra, Marta|||0000-0002-7895-8337
author_role author
author2 Mas Moruno, Carlos|||0000-0001-8337-0872
Martín Gómez, Helena|||0000-0001-8956-116X
Pino Curto, Marc
Díaz Ricart, Maribel
Escolar Albaladejo, Ginés
Ginebra Molins, Maria Pau|||0000-0002-4700-5621
Pegueroles Neyra, Marta|||0000-0002-7895-8337
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biomedical materials
Materials biomèdics
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
topic Biomedical materials
Materials biomèdics
Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomaterials
description Biomimetic surface modification with cell-adhesive peptides is a promising approach to improve endothelialization of current bioresorbable stents (BRS). Among them, RGDS and YIGSR sequences have been reported to mediate adhesion and migration of endothelial cells (ECs) while preventing platelet activation. This work presents the functionalization of novel 3D-printed poly-L-lactic acid (PLLA) and poly(L-lactic-co-e-caprolactone) (PLCL) BRS with linear RGDS and YIGSR sequences, as well as a dual platform (PF) containing both motifs within a single biomolecule. Functionalized surfaces were characterized in terms of static contact angle, biomolecule distribution under confocal fluorescence microscopy and peptide quantification via detachment from the surface, showing a biomolecule density in the range of 0.5 to 3.5 nmol cm-2. Biological evaluation comprised a cell adhesion test on functionalized films with ECs and a blood perfusion assay on functionalized stents to assess ECs response and device hemocompatibility, respectively. Cell adhesion assays evidenced significantly increased cell number and spreading onto functionalized films with respect to control samples. Regarding stents’ hemocompatibility, platelet adhesion onto PLCL stents was severely decreased with respect to PLLA. In addition, functionalization with RGDS, YIGSR and the PF rendered BRS stents displaying even further reduced platelet adhesion. In conclusion, the combination of intrinsically less prothrombogenic materials such as PLCL and its functionalization with EC-discriminating adhesive biomolecules paves the way for a new generation of BRS based on accelerated re-endothelialization approaches.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-05-08
2023
2023-11-30
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
AM
http://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/2117/397428
https://dx.doi.org/10.1039/d3bm00458a
url https://hdl.handle.net/2117/397428
https://dx.doi.org/10.1039/d3bm00458a
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry (RSC)
publisher.none.fl_str_mv Royal Society of Chemistry (RSC)
dc.source.none.fl_str_mv reponame:UPCommons. Portal del coneixement obert de la UPC
instname:Universitat Politècnica de Catalunya (UPC)
instname_str Universitat Politècnica de Catalunya (UPC)
reponame_str UPCommons. Portal del coneixement obert de la UPC
collection UPCommons. Portal del coneixement obert de la UPC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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