MicroRNA-199b deregulation shows oncogenic properties and promising clinical value as circulating marker in locally advanced rectal cancer patients

The identification of robust prognostic markers still represents a need in locally advanced rectal cancer (LARC). MicroRNAs (miRs) have progressively emerged as promising circulating markers, overcoming some limitations that traditional biopsy comprises. Tissue miR-199b deregulation has been reporte...

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Detalhes bibliográficos
Autores: Santos, Andrea, Cristóbal, Ion, Rubio, Jaime, Caramés, Cristina, Luque, Melani, Sanz-Álvarez, Marta, Morales Gallego, Miriam, Madoz-Gúrpide, Juan, Rojo, Federico, García-Foncillas López, Jesús Miguel
Tipo de documento: artigo
Data de publicação:2022
País:España
Recursos:Universidad Autónoma de Madrid
Repositório:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglês
OAI Identifier:oai:repositorio.uam.es:10486/712456
Acesso em linha:http://hdl.handle.net/10486/712456
https://dx.doi.org/10.3390/ijms23042203
Access Level:Acceso aberto
Palavra-chave:MiR-199b
tumor suppressor
prognosis
LARC
Medicina
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spelling MicroRNA-199b deregulation shows oncogenic properties and promising clinical value as circulating marker in locally advanced rectal cancer patientsSantos, AndreaCristóbal, IonRubio, JaimeCaramés, CristinaLuque, MelaniSanz-Álvarez, MartaMorales Gallego, MiriamMadoz-Gúrpide, JuanRojo, FedericoGarcía-Foncillas López, Jesús MiguelMiR-199btumor suppressorprognosisLARCMedicinaThe identification of robust prognostic markers still represents a need in locally advanced rectal cancer (LARC). MicroRNAs (miRs) have progressively emerged as promising circulating markers, overcoming some limitations that traditional biopsy comprises. Tissue miR-199b deregulation has been reported to predict outcome and response to neoadjuvant chemoradiotherapy (nCRT) in LARC, and was also found to be associated with disease progression in colorectal cancer. However, its biological and clinical relevance remains to be fully clarified. Thus, we observed here that miR-199b regulates cell migration, aggressiveness, and cell growth, and inhibits colonosphere formation and induces caspase-dependent apoptosis. Moreover, miR-199b expression was quantified by real-time PCR in plasma samples from LARC patients and its downregulation was observed in 22.7% of cases. This alteration was found to be associated with higher tumor size (p = 0.002) and pathological stage (p = 0.020) after nCRT. Notably, we observed substantially lower global miR-199b expression associated with patient downstaging (p = 0.009), as well as in non-responders compared to those cases who responded to nCRT in both pre- (p = 0.003) and post-treatment samples (p = 0.038). In concordance, we found that miR-199b served as a predictor marker of response to neoadjuvant therapy in our cohort (p = 0.011). Altogether, our findings here demonstrate the functional relevance of miR-199b in this disease and its potential value as a novel circulating marker in LARCThis research was funded by PI18/00382 and PI16/01468 grants from “Instituto de Salud Carlos III FEDER”. M.S.-A. is supported by “Fundación Conchita Rábago de Jiménez Díaz”MDPIDepartamento de MedicinaFacultad de Medicina20222022-02-17research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/712456https://dx.doi.org/10.3390/ijms23042203reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7124562026-06-23T12:46:27Z
dc.title.none.fl_str_mv MicroRNA-199b deregulation shows oncogenic properties and promising clinical value as circulating marker in locally advanced rectal cancer patients
title MicroRNA-199b deregulation shows oncogenic properties and promising clinical value as circulating marker in locally advanced rectal cancer patients
spellingShingle MicroRNA-199b deregulation shows oncogenic properties and promising clinical value as circulating marker in locally advanced rectal cancer patients
Santos, Andrea
MiR-199b
tumor suppressor
prognosis
LARC
Medicina
title_short MicroRNA-199b deregulation shows oncogenic properties and promising clinical value as circulating marker in locally advanced rectal cancer patients
title_full MicroRNA-199b deregulation shows oncogenic properties and promising clinical value as circulating marker in locally advanced rectal cancer patients
title_fullStr MicroRNA-199b deregulation shows oncogenic properties and promising clinical value as circulating marker in locally advanced rectal cancer patients
title_full_unstemmed MicroRNA-199b deregulation shows oncogenic properties and promising clinical value as circulating marker in locally advanced rectal cancer patients
title_sort MicroRNA-199b deregulation shows oncogenic properties and promising clinical value as circulating marker in locally advanced rectal cancer patients
dc.creator.none.fl_str_mv Santos, Andrea
Cristóbal, Ion
Rubio, Jaime
Caramés, Cristina
Luque, Melani
Sanz-Álvarez, Marta
Morales Gallego, Miriam
Madoz-Gúrpide, Juan
Rojo, Federico
García-Foncillas López, Jesús Miguel
author Santos, Andrea
author_facet Santos, Andrea
Cristóbal, Ion
Rubio, Jaime
Caramés, Cristina
Luque, Melani
Sanz-Álvarez, Marta
Morales Gallego, Miriam
Madoz-Gúrpide, Juan
Rojo, Federico
García-Foncillas López, Jesús Miguel
author_role author
author2 Cristóbal, Ion
Rubio, Jaime
Caramés, Cristina
Luque, Melani
Sanz-Álvarez, Marta
Morales Gallego, Miriam
Madoz-Gúrpide, Juan
Rojo, Federico
García-Foncillas López, Jesús Miguel
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Medicina
Facultad de Medicina
dc.subject.none.fl_str_mv MiR-199b
tumor suppressor
prognosis
LARC
Medicina
topic MiR-199b
tumor suppressor
prognosis
LARC
Medicina
description The identification of robust prognostic markers still represents a need in locally advanced rectal cancer (LARC). MicroRNAs (miRs) have progressively emerged as promising circulating markers, overcoming some limitations that traditional biopsy comprises. Tissue miR-199b deregulation has been reported to predict outcome and response to neoadjuvant chemoradiotherapy (nCRT) in LARC, and was also found to be associated with disease progression in colorectal cancer. However, its biological and clinical relevance remains to be fully clarified. Thus, we observed here that miR-199b regulates cell migration, aggressiveness, and cell growth, and inhibits colonosphere formation and induces caspase-dependent apoptosis. Moreover, miR-199b expression was quantified by real-time PCR in plasma samples from LARC patients and its downregulation was observed in 22.7% of cases. This alteration was found to be associated with higher tumor size (p = 0.002) and pathological stage (p = 0.020) after nCRT. Notably, we observed substantially lower global miR-199b expression associated with patient downstaging (p = 0.009), as well as in non-responders compared to those cases who responded to nCRT in both pre- (p = 0.003) and post-treatment samples (p = 0.038). In concordance, we found that miR-199b served as a predictor marker of response to neoadjuvant therapy in our cohort (p = 0.011). Altogether, our findings here demonstrate the functional relevance of miR-199b in this disease and its potential value as a novel circulating marker in LARC
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-02-17
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/712456
https://dx.doi.org/10.3390/ijms23042203
url http://hdl.handle.net/10486/712456
https://dx.doi.org/10.3390/ijms23042203
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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