Tomato Systemin induces resistance against Plectosphaerella cucumerina in Arabidopsis through the induction of phenolic compounds and priming of tryptophan derivatives

Phytocytokines are endogenous danger peptides that are actively released after a pest or pathogen attack, triggering an amplification of plant immune responses. Here, we found that Systemin, a peptide from tomato, has a substantial impact at the molecular level in Arabidopsis plants that leads to in...

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Autores: Pastor-Fernández, Julia, Sánchez-Bel, P., Gamir, Jordi, Pastor, Victoria, Sanmartín, N., Cerezo García, Miguel, Andrés-Moreno, S., Flors, Victor
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/279511
Acesso em linha:http://hdl.handle.net/10261/279511
Access Level:Acceso aberto
Palavra-chave:Arabidopsis
Indolic compounds
Induced resistance
Phytocytokines
Plectosphaerella cucumerina
Systemin
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spelling Tomato Systemin induces resistance against Plectosphaerella cucumerina in Arabidopsis through the induction of phenolic compounds and priming of tryptophan derivativesPastor-Fernández, JuliaSánchez-Bel, P.Gamir, JordiPastor, VictoriaSanmartín, N.Cerezo García, MiguelAndrés-Moreno, S.Flors, VictorArabidopsisIndolic compoundsInduced resistancePhytocytokinesPlectosphaerella cucumerinaSysteminPhytocytokines are endogenous danger peptides that are actively released after a pest or pathogen attack, triggering an amplification of plant immune responses. Here, we found that Systemin, a peptide from tomato, has a substantial impact at the molecular level in Arabidopsis plants that leads to induced resistance against Plectosphaerella cucumerina. Using transcriptional and metabolomics approaches, and loss-of-function mutants to analyse the molecular mechanisms underlying induced resistance against the necrotroph, we decipher the enhanced molecular responses in Systemin-treated plants following infection. Some protein complexes involved in the response to other damage signals, including the BAK1-BIK1 protein complex and heterotrimeric G proteins, as well as MPK activation, were among the early signalling events triggered by Systemin in Arabidopsis upon infection. Non-targeted analysis of the late responses underlying Systemin-Induced Resistance (Sys-IR) showed that phenolic and indolic compounds were the most representative groups in the Systemin metabolic fingerprint. Lack of flavonoids resulted in the impairment of Sys-IR. On the other hand, some indolic compounds showed a priming profile and were also essential for functional Sys-IR. Evidence presented here shows that plants can sense heterologous peptides from other species as danger signals driving the participation of common protein cascades activated in the PTI and promoting enhanced resistance against necrotrophic fungus.This work was supported by grant RTI2018-094350-B-C33 from the Spanish National R&D Plan of the Ministry of Science, Innovation and Universities (MICIU) and the European Regional Development Fund (ERDF). The Plan de Promoción de la investigación with the grant UJI-A2019-05 to JP and VP and the fellowship CDEIGENT/2018/015 from Generalitat Valenciana that granted JG. We also thank the SCIC of the Universitat Jaume I where the LC-MS analysis were performed and the grant of JPF, PREDOC/2018/20 from the “Plan Propio de Investigación Universitat Jaume I”.ElsevierMinisterio de Ciencia e Innovación (España)European CommissionGeneralitat ValencianaUniversidad Jaime IConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/279511reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094350-B-C33http://dx.doi.org/10.1016/j.plantsci.2022.111321Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2795112026-05-22T06:33:51Z
dc.title.none.fl_str_mv Tomato Systemin induces resistance against Plectosphaerella cucumerina in Arabidopsis through the induction of phenolic compounds and priming of tryptophan derivatives
title Tomato Systemin induces resistance against Plectosphaerella cucumerina in Arabidopsis through the induction of phenolic compounds and priming of tryptophan derivatives
spellingShingle Tomato Systemin induces resistance against Plectosphaerella cucumerina in Arabidopsis through the induction of phenolic compounds and priming of tryptophan derivatives
Pastor-Fernández, Julia
Arabidopsis
Indolic compounds
Induced resistance
Phytocytokines
Plectosphaerella cucumerina
Systemin
title_short Tomato Systemin induces resistance against Plectosphaerella cucumerina in Arabidopsis through the induction of phenolic compounds and priming of tryptophan derivatives
title_full Tomato Systemin induces resistance against Plectosphaerella cucumerina in Arabidopsis through the induction of phenolic compounds and priming of tryptophan derivatives
title_fullStr Tomato Systemin induces resistance against Plectosphaerella cucumerina in Arabidopsis through the induction of phenolic compounds and priming of tryptophan derivatives
title_full_unstemmed Tomato Systemin induces resistance against Plectosphaerella cucumerina in Arabidopsis through the induction of phenolic compounds and priming of tryptophan derivatives
title_sort Tomato Systemin induces resistance against Plectosphaerella cucumerina in Arabidopsis through the induction of phenolic compounds and priming of tryptophan derivatives
dc.creator.none.fl_str_mv Pastor-Fernández, Julia
Sánchez-Bel, P.
Gamir, Jordi
Pastor, Victoria
Sanmartín, N.
Cerezo García, Miguel
Andrés-Moreno, S.
Flors, Victor
author Pastor-Fernández, Julia
author_facet Pastor-Fernández, Julia
Sánchez-Bel, P.
Gamir, Jordi
Pastor, Victoria
Sanmartín, N.
Cerezo García, Miguel
Andrés-Moreno, S.
Flors, Victor
author_role author
author2 Sánchez-Bel, P.
Gamir, Jordi
Pastor, Victoria
Sanmartín, N.
Cerezo García, Miguel
Andrés-Moreno, S.
Flors, Victor
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
European Commission
Generalitat Valenciana
Universidad Jaime I
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Arabidopsis
Indolic compounds
Induced resistance
Phytocytokines
Plectosphaerella cucumerina
Systemin
topic Arabidopsis
Indolic compounds
Induced resistance
Phytocytokines
Plectosphaerella cucumerina
Systemin
description Phytocytokines are endogenous danger peptides that are actively released after a pest or pathogen attack, triggering an amplification of plant immune responses. Here, we found that Systemin, a peptide from tomato, has a substantial impact at the molecular level in Arabidopsis plants that leads to induced resistance against Plectosphaerella cucumerina. Using transcriptional and metabolomics approaches, and loss-of-function mutants to analyse the molecular mechanisms underlying induced resistance against the necrotroph, we decipher the enhanced molecular responses in Systemin-treated plants following infection. Some protein complexes involved in the response to other damage signals, including the BAK1-BIK1 protein complex and heterotrimeric G proteins, as well as MPK activation, were among the early signalling events triggered by Systemin in Arabidopsis upon infection. Non-targeted analysis of the late responses underlying Systemin-Induced Resistance (Sys-IR) showed that phenolic and indolic compounds were the most representative groups in the Systemin metabolic fingerprint. Lack of flavonoids resulted in the impairment of Sys-IR. On the other hand, some indolic compounds showed a priming profile and were also essential for functional Sys-IR. Evidence presented here shows that plants can sense heterologous peptides from other species as danger signals driving the participation of common protein cascades activated in the PTI and promoting enhanced resistance against necrotrophic fungus.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/279511
url http://hdl.handle.net/10261/279511
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094350-B-C33
http://dx.doi.org/10.1016/j.plantsci.2022.111321
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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