Biorefinery of the relevant brown algae Saccharina latissima and Ascophyllum nodosum towards novel, sustainable and functional ingredients

This doctoral thesis aims to provide fundamental and practical knowledge on the valorization of brown seaweeds. It has sought to understand the relationship between the structure and functionality of their polysaccharides and proteins, considering their functional, technological and bioactive proper...

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Author: Bojorges, Hylenne
Format: doctoral thesis
Publication Date:2024
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/387021
Online Access:http://hdl.handle.net/10261/387021
Access Level:Open access
Keyword:Polysaccharides
Algal proteins
Macroalgae
Brown seaweed
Waste streams
Valorizarion
Functional properties
Bioactive properties
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
Objetivo 12: Garantizar modalidades de consumo y producción sostenibles
Objetivo 13: Adoptar medidas urgentes para combatir el cambio climático y sus efectos
Objetivo 14: Conservar y utilizar sosteniblemente los océanos, los mares y los recursos marinos
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oai_identifier_str oai:digital.csic.es:10261/387021
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Biorefinery of the relevant brown algae Saccharina latissima and Ascophyllum nodosum towards novel, sustainable and functional ingredients
title Biorefinery of the relevant brown algae Saccharina latissima and Ascophyllum nodosum towards novel, sustainable and functional ingredients
spellingShingle Biorefinery of the relevant brown algae Saccharina latissima and Ascophyllum nodosum towards novel, sustainable and functional ingredients
Bojorges, Hylenne
Polysaccharides
Algal proteins
Macroalgae
Brown seaweed
Waste streams
Valorizarion
Functional properties
Bioactive properties
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
Objetivo 12: Garantizar modalidades de consumo y producción sostenibles
Objetivo 13: Adoptar medidas urgentes para combatir el cambio climático y sus efectos
Objetivo 14: Conservar y utilizar sosteniblemente los océanos, los mares y los recursos marinos
title_short Biorefinery of the relevant brown algae Saccharina latissima and Ascophyllum nodosum towards novel, sustainable and functional ingredients
title_full Biorefinery of the relevant brown algae Saccharina latissima and Ascophyllum nodosum towards novel, sustainable and functional ingredients
title_fullStr Biorefinery of the relevant brown algae Saccharina latissima and Ascophyllum nodosum towards novel, sustainable and functional ingredients
title_full_unstemmed Biorefinery of the relevant brown algae Saccharina latissima and Ascophyllum nodosum towards novel, sustainable and functional ingredients
title_sort Biorefinery of the relevant brown algae Saccharina latissima and Ascophyllum nodosum towards novel, sustainable and functional ingredients
dc.creator.none.fl_str_mv Bojorges, Hylenne
author Bojorges, Hylenne
author_facet Bojorges, Hylenne
author_role author
dc.contributor.none.fl_str_mv López-Rubio, Amparo
Fabra, María José
Martínez-Abad, Antonio
Generalitat Valenciana
Bojorges, Hylenne [0000-0002-8811-8665]
López-Rubio, Amparo [0000-0001-6469-9402]
Fabra, María José [0000-0001-6726-4455]
Martínez-Abad, Antonio [0000-0002-6656-2917]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Polysaccharides
Algal proteins
Macroalgae
Brown seaweed
Waste streams
Valorizarion
Functional properties
Bioactive properties
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
Objetivo 12: Garantizar modalidades de consumo y producción sostenibles
Objetivo 13: Adoptar medidas urgentes para combatir el cambio climático y sus efectos
Objetivo 14: Conservar y utilizar sosteniblemente los océanos, los mares y los recursos marinos
topic Polysaccharides
Algal proteins
Macroalgae
Brown seaweed
Waste streams
Valorizarion
Functional properties
Bioactive properties
http://metadata.un.org/sdg/12
Ensure sustainable consumption and production patterns
Objetivo 12: Garantizar modalidades de consumo y producción sostenibles
Objetivo 13: Adoptar medidas urgentes para combatir el cambio climático y sus efectos
Objetivo 14: Conservar y utilizar sosteniblemente los océanos, los mares y los recursos marinos
description This doctoral thesis aims to provide fundamental and practical knowledge on the valorization of brown seaweeds. It has sought to understand the relationship between the structure and functionality of their polysaccharides and proteins, considering their functional, technological and bioactive properties. All this has been approached from a biorefinery perspective, focused on circular economy and sustainability. First, an optimization for extracting valuable compounds from brown seaweeds was carried out using various innovative technologies. A high-pressure pre-treatment of brown seaweeds (Ascophyllum nodosum and Saccharina latissima) to extract alginate was investigated and compared with the conventional extraction method. The purpose was to understand how initial differences in algal cell wall composition and structure affected the extraction of this polysaccharide and its technological properties, such as its ability as emulsifier and gelling agent. Likewise, the extraction of protein-polysaccharide conjugates was examined by sequential application of several extraction methods, as well as their functional properties (water, oil retention activity and emulsifying capacity) and bioactive properties (antioxidant activity and antihypertensive capacity). In these studies, differences were observed in the cell wall architecture of the algae, belonging to different orders, such as Fucales and Laminariales, which directly influenced the extracted compounds and their structural and technological properties. However, despite these differences, the results showed the potential of the extracted compounds as ingredients in the food and pharmaceutical industries. The second section of this thesis explored the valorization of by-products generated during alginate extraction. An exhaustive study of all the fractions and by-products produced in this process was carried out to identify the most promising fractions for valorization. The results showed that solid wastes had the most significant potential due to their physicochemical composition. Therefore, possible strategies to valorize these wastes were suggested, highlighting their high potential as bioactive, texturizing, or nutritional value-added ingredients for food, feed, cosmetic, and pharmaceutical applications. Besides, from this work, sequential and combined extraction by different methods was investigated to recover as much intracellular protein as possible from these solid wastes. It was found that the protein fraction of the extracts contained a high percentage of essential amino acids, adequate functional properties, and an interesting antihypertensive activity. Finally, the third part of this thesis focused on generating knowledge through the structural elucidation of alginate. For this purpose, a rapid method was developed to determine the M/G ratio of alginate, which showed values close to the reference method (1H NMR), demonstrating its accuracy. Overall, this PhD thesis represents a significant advance in generating knowledge and valorizing algal biomass and its residues into high-value compounds. Furthermore, these approaches are potentially applicable to other algal biomasses.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
http://purl.org/coar/resource_type/c_db06
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/387021
url http://hdl.handle.net/10261/387021
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404049815633920
spelling Biorefinery of the relevant brown algae Saccharina latissima and Ascophyllum nodosum towards novel, sustainable and functional ingredientsBojorges, HylennePolysaccharidesAlgal proteinsMacroalgaeBrown seaweedWaste streamsValorizarionFunctional propertiesBioactive propertieshttp://metadata.un.org/sdg/12Ensure sustainable consumption and production patternsObjetivo 12: Garantizar modalidades de consumo y producción sosteniblesObjetivo 13: Adoptar medidas urgentes para combatir el cambio climático y sus efectosObjetivo 14: Conservar y utilizar sosteniblemente los océanos, los mares y los recursos marinosThis doctoral thesis aims to provide fundamental and practical knowledge on the valorization of brown seaweeds. It has sought to understand the relationship between the structure and functionality of their polysaccharides and proteins, considering their functional, technological and bioactive properties. All this has been approached from a biorefinery perspective, focused on circular economy and sustainability. First, an optimization for extracting valuable compounds from brown seaweeds was carried out using various innovative technologies. A high-pressure pre-treatment of brown seaweeds (Ascophyllum nodosum and Saccharina latissima) to extract alginate was investigated and compared with the conventional extraction method. The purpose was to understand how initial differences in algal cell wall composition and structure affected the extraction of this polysaccharide and its technological properties, such as its ability as emulsifier and gelling agent. Likewise, the extraction of protein-polysaccharide conjugates was examined by sequential application of several extraction methods, as well as their functional properties (water, oil retention activity and emulsifying capacity) and bioactive properties (antioxidant activity and antihypertensive capacity). In these studies, differences were observed in the cell wall architecture of the algae, belonging to different orders, such as Fucales and Laminariales, which directly influenced the extracted compounds and their structural and technological properties. However, despite these differences, the results showed the potential of the extracted compounds as ingredients in the food and pharmaceutical industries. The second section of this thesis explored the valorization of by-products generated during alginate extraction. An exhaustive study of all the fractions and by-products produced in this process was carried out to identify the most promising fractions for valorization. The results showed that solid wastes had the most significant potential due to their physicochemical composition. Therefore, possible strategies to valorize these wastes were suggested, highlighting their high potential as bioactive, texturizing, or nutritional value-added ingredients for food, feed, cosmetic, and pharmaceutical applications. Besides, from this work, sequential and combined extraction by different methods was investigated to recover as much intracellular protein as possible from these solid wastes. It was found that the protein fraction of the extracts contained a high percentage of essential amino acids, adequate functional properties, and an interesting antihypertensive activity. Finally, the third part of this thesis focused on generating knowledge through the structural elucidation of alginate. For this purpose, a rapid method was developed to determine the M/G ratio of alginate, which showed values close to the reference method (1H NMR), demonstrating its accuracy. Overall, this PhD thesis represents a significant advance in generating knowledge and valorizing algal biomass and its residues into high-value compounds. Furthermore, these approaches are potentially applicable to other algal biomasses.Generalitat Valenciana for the award of a Santiago Grisolía grant (GRISOLIAP/2019/007).Peer reviewedLópez-Rubio, AmparoFabra, María JoséMartínez-Abad, AntonioGeneralitat ValencianaBojorges, Hylenne [0000-0002-8811-8665]López-Rubio, Amparo [0000-0001-6469-9402]Fabra, María José [0000-0001-6726-4455]Martínez-Abad, Antonio [0000-0002-6656-2917]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/doctoralThesishttp://purl.org/coar/resource_type/c_db06http://hdl.handle.net/10261/387021reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3870212026-05-22T06:33:51Z
score 15,811543

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