Genome-wide chromatin occupancy reveals a role for ASH2 in transcriptional pausing      

While it is widely acknowledged that the ubiquitin-proteasome system plays an important role in transcription, little is known concerning the mechanistic basis, in particular the spatial organization of proteasome-dependent proteolysis at the transcription site. Here, we show that proteasomal activi...

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Autores: Pérez-Lluch, Sílvia, Blanco García, Enrique, Carbonell Sanroma, Albert, Raha, D., Snyder, Michael P., Serras Rigalt, Florenci, Corominas, Montserrat (Corominas Guiu)
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2011
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/34231
Acceso en línea:https://hdl.handle.net/2445/34231
Access Level:acceso abierto
Palabra clave:Expressió gènica
Drosòfila
Mutació (Biologia)
Gene expression
Drosophila
Mutation (Biology)
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spelling Genome-wide chromatin occupancy reveals a role for ASH2 in transcriptional pausing      Pérez-Lluch, SílviaBlanco García, EnriqueCarbonell Sanroma, AlbertRaha, D.Snyder, Michael P.Serras Rigalt, FlorenciCorominas, Montserrat (Corominas Guiu)Expressió gènicaDrosòfilaMutació (Biologia)Gene expressionDrosophilaMutation (Biology)While it is widely acknowledged that the ubiquitin-proteasome system plays an important role in transcription, little is known concerning the mechanistic basis, in particular the spatial organization of proteasome-dependent proteolysis at the transcription site. Here, we show that proteasomal activity and tetraubiquitinated proteins concentrate to nucleoplasmic microenvironments in the euchromatin. Such proteolytic domains are immobile and distinctly positioned in relation to transcriptional processes. Analysis of gene arrays and early genes in Caenorhabditis elegans embryos reveals that proteasomes and proteasomal activity are distantly located relative to transcriptionally active genes. In contrast, transcriptional inhibition generally induces local overlap of proteolytic microdomains with components of the transcription machinery and degradation of RNA polymerase II. The results establish that spatial organization of proteasomal activity differs with respect to distinct phases of the transcription cycle in at least some genes, and thus might contribute to the plasticity of gene expression in response to environmental stimuli.Oxford University Press2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/34231Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1093/nar/gkq1322Nucleic Acids Research, 2011, vol. 39, num. 11, p. 4628-4639http://dx.doi.org/10.1093/nar/gkq1322cc-by-nc (c) Perez-Lluch, Silvia et al., 2011http://creativecommons.org/licenses/by-nc/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/342312026-05-27T06:46:51Z
dc.title.none.fl_str_mv Genome-wide chromatin occupancy reveals a role for ASH2 in transcriptional pausing      
title Genome-wide chromatin occupancy reveals a role for ASH2 in transcriptional pausing      
spellingShingle Genome-wide chromatin occupancy reveals a role for ASH2 in transcriptional pausing      
Pérez-Lluch, Sílvia
Expressió gènica
Drosòfila
Mutació (Biologia)
Gene expression
Drosophila
Mutation (Biology)
title_short Genome-wide chromatin occupancy reveals a role for ASH2 in transcriptional pausing      
title_full Genome-wide chromatin occupancy reveals a role for ASH2 in transcriptional pausing      
title_fullStr Genome-wide chromatin occupancy reveals a role for ASH2 in transcriptional pausing      
title_full_unstemmed Genome-wide chromatin occupancy reveals a role for ASH2 in transcriptional pausing      
title_sort Genome-wide chromatin occupancy reveals a role for ASH2 in transcriptional pausing      
dc.creator.none.fl_str_mv Pérez-Lluch, Sílvia
Blanco García, Enrique
Carbonell Sanroma, Albert
Raha, D.
Snyder, Michael P.
Serras Rigalt, Florenci
Corominas, Montserrat (Corominas Guiu)
author Pérez-Lluch, Sílvia
author_facet Pérez-Lluch, Sílvia
Blanco García, Enrique
Carbonell Sanroma, Albert
Raha, D.
Snyder, Michael P.
Serras Rigalt, Florenci
Corominas, Montserrat (Corominas Guiu)
author_role author
author2 Blanco García, Enrique
Carbonell Sanroma, Albert
Raha, D.
Snyder, Michael P.
Serras Rigalt, Florenci
Corominas, Montserrat (Corominas Guiu)
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Expressió gènica
Drosòfila
Mutació (Biologia)
Gene expression
Drosophila
Mutation (Biology)
topic Expressió gènica
Drosòfila
Mutació (Biologia)
Gene expression
Drosophila
Mutation (Biology)
description While it is widely acknowledged that the ubiquitin-proteasome system plays an important role in transcription, little is known concerning the mechanistic basis, in particular the spatial organization of proteasome-dependent proteolysis at the transcription site. Here, we show that proteasomal activity and tetraubiquitinated proteins concentrate to nucleoplasmic microenvironments in the euchromatin. Such proteolytic domains are immobile and distinctly positioned in relation to transcriptional processes. Analysis of gene arrays and early genes in Caenorhabditis elegans embryos reveals that proteasomes and proteasomal activity are distantly located relative to transcriptionally active genes. In contrast, transcriptional inhibition generally induces local overlap of proteolytic microdomains with components of the transcription machinery and degradation of RNA polymerase II. The results establish that spatial organization of proteasomal activity differs with respect to distinct phases of the transcription cycle in at least some genes, and thus might contribute to the plasticity of gene expression in response to environmental stimuli.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/34231
url https://hdl.handle.net/2445/34231
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1093/nar/gkq1322
Nucleic Acids Research, 2011, vol. 39, num. 11, p. 4628-4639
http://dx.doi.org/10.1093/nar/gkq1322
dc.rights.none.fl_str_mv cc-by-nc (c) Perez-Lluch, Silvia et al., 2011
http://creativecommons.org/licenses/by-nc/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc (c) Perez-Lluch, Silvia et al., 2011
http://creativecommons.org/licenses/by-nc/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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