Association between long-term air pollution exposure and DNA methylation: The REGICOR study
INTRODUCTION: Limited evidence suggests that epigenetic mechanisms may partially mediate the adverse effects of air pollution on health. Our aims were to identify new genomic loci showing differential DNA methylation associated with long-term exposure to air pollution and to replicate loci previousl...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:10230/43157 |
| Acceso en línea: | http://hdl.handle.net/10230/43157 http://dx.doi.org/10.1016/j.envres.2019.108550 |
| Access Level: | acceso abierto |
| Palabra clave: | Air pollution DNA methylation Epigenome-wide association study |
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Association between long-term air pollution exposure and DNA methylation: The REGICOR studySayols, SergiFernández Sanlés, Alba, 1988-Prats Uribe, AlbertSubirana Cachinero, IsaacPlusquin, MichelleKünzli, NinoMarrugat, Jaume, 1954-Basagaña Flores, XavierElosua Llanos, RobertoAir pollutionDNA methylationEpigenome-wide association studyINTRODUCTION: Limited evidence suggests that epigenetic mechanisms may partially mediate the adverse effects of air pollution on health. Our aims were to identify new genomic loci showing differential DNA methylation associated with long-term exposure to air pollution and to replicate loci previously identified in other studies. METHODS: A two-stage epigenome-wide association study was designed: 630 individuals from the REGICOR study were included in the discovery and 454 participants of the EPIC-Italy study in the validation stage. DNA methylation was assessed using the Infinium HumanMethylation450 BeadChip. NOX, NO2, PM10, PM2.5, PMcoarse, traffic intensity and traffic load exposure were measured according to the ESCAPE protocol. A systematic review was undertaken to identify those cytosine-phosphate-guanine (CpGs) associated with air pollution in previous studies and we screened for them in the discovery study. RESULTS: In the discovery stage of the epigenome-wide association study, 81 unique CpGs were associated with air pollution (p-value <10-5) but none of them were validated in the replication sample. Furthermore, we identified 15 CpGs in the systematic review showing differential methylation with a p-value fulfilling the Bonferroni criteria and 1673 CpGs fulfilling the false discovery rate criteria, all of which were related to PM2.5 or NO2. None of them was replicated in the discovery study, in which the top hits were located in an intergenic region on chromosome 1 (cg10893043, p-value = 6.79·10-5) and in the LRRC45 and PXK genes (cg05088605, p-value = 2.15·10-04; cg16560256, p-value = 2.23·10-04). CONCLUSIONS: Neither new genomic loci associated with long-term air pollution were identified, nor previously identified loci were replicated. Continued efforts to test this potential association are warranted.This work was supported by grants from the Catalan Agència de Gestió d’Ajuts Universitaris de Recerca (2017-SGR-222); Departament de Salut, Generalitat de Catalunya (PERIS) (SLT002/0088); Spanish Ministry of Economy through the Instituto de Salud Carlos III-FEDER (FIS PI18/00017, PI15/00051, PI12/00232, CIBERCV, CIBERESP, Red de Investigación Cardiovascular RD12/0042). S.S-B. was funded by contracts from Instituto de Salud Carlos III-FEDER (IFI14/00007). A.F-S. was funded by the Spanish Ministry of Economy and Competitiveness (BES-2014-069718).Elsevier20192019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/43157http://dx.doi.org/10.1016/j.envres.2019.108550reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésEnvironmental Research. 2019;176:108550info:eu-repo/grantAgreement/ES/1PE/BES2014-069718© Elsevier http://dx.doi.org/10.1016/j.envres.2019.108550info:eu-repo/semantics/openAccessoai:recercat.cat:10230/431572026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Association between long-term air pollution exposure and DNA methylation: The REGICOR study |
| title |
Association between long-term air pollution exposure and DNA methylation: The REGICOR study |
| spellingShingle |
Association between long-term air pollution exposure and DNA methylation: The REGICOR study Sayols, Sergi Air pollution DNA methylation Epigenome-wide association study |
| title_short |
Association between long-term air pollution exposure and DNA methylation: The REGICOR study |
| title_full |
Association between long-term air pollution exposure and DNA methylation: The REGICOR study |
| title_fullStr |
Association between long-term air pollution exposure and DNA methylation: The REGICOR study |
| title_full_unstemmed |
Association between long-term air pollution exposure and DNA methylation: The REGICOR study |
| title_sort |
Association between long-term air pollution exposure and DNA methylation: The REGICOR study |
| dc.creator.none.fl_str_mv |
Sayols, Sergi Fernández Sanlés, Alba, 1988- Prats Uribe, Albert Subirana Cachinero, Isaac Plusquin, Michelle Künzli, Nino Marrugat, Jaume, 1954- Basagaña Flores, Xavier Elosua Llanos, Roberto |
| author |
Sayols, Sergi |
| author_facet |
Sayols, Sergi Fernández Sanlés, Alba, 1988- Prats Uribe, Albert Subirana Cachinero, Isaac Plusquin, Michelle Künzli, Nino Marrugat, Jaume, 1954- Basagaña Flores, Xavier Elosua Llanos, Roberto |
| author_role |
author |
| author2 |
Fernández Sanlés, Alba, 1988- Prats Uribe, Albert Subirana Cachinero, Isaac Plusquin, Michelle Künzli, Nino Marrugat, Jaume, 1954- Basagaña Flores, Xavier Elosua Llanos, Roberto |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Air pollution DNA methylation Epigenome-wide association study |
| topic |
Air pollution DNA methylation Epigenome-wide association study |
| description |
INTRODUCTION: Limited evidence suggests that epigenetic mechanisms may partially mediate the adverse effects of air pollution on health. Our aims were to identify new genomic loci showing differential DNA methylation associated with long-term exposure to air pollution and to replicate loci previously identified in other studies. METHODS: A two-stage epigenome-wide association study was designed: 630 individuals from the REGICOR study were included in the discovery and 454 participants of the EPIC-Italy study in the validation stage. DNA methylation was assessed using the Infinium HumanMethylation450 BeadChip. NOX, NO2, PM10, PM2.5, PMcoarse, traffic intensity and traffic load exposure were measured according to the ESCAPE protocol. A systematic review was undertaken to identify those cytosine-phosphate-guanine (CpGs) associated with air pollution in previous studies and we screened for them in the discovery study. RESULTS: In the discovery stage of the epigenome-wide association study, 81 unique CpGs were associated with air pollution (p-value <10-5) but none of them were validated in the replication sample. Furthermore, we identified 15 CpGs in the systematic review showing differential methylation with a p-value fulfilling the Bonferroni criteria and 1673 CpGs fulfilling the false discovery rate criteria, all of which were related to PM2.5 or NO2. None of them was replicated in the discovery study, in which the top hits were located in an intergenic region on chromosome 1 (cg10893043, p-value = 6.79·10-5) and in the LRRC45 and PXK genes (cg05088605, p-value = 2.15·10-04; cg16560256, p-value = 2.23·10-04). CONCLUSIONS: Neither new genomic loci associated with long-term air pollution were identified, nor previously identified loci were replicated. Continued efforts to test this potential association are warranted. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2019 2019 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
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article |
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acceptedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10230/43157 http://dx.doi.org/10.1016/j.envres.2019.108550 |
| url |
http://hdl.handle.net/10230/43157 http://dx.doi.org/10.1016/j.envres.2019.108550 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Environmental Research. 2019;176:108550 info:eu-repo/grantAgreement/ES/1PE/BES2014-069718 |
| dc.rights.none.fl_str_mv |
© Elsevier http://dx.doi.org/10.1016/j.envres.2019.108550 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
© Elsevier http://dx.doi.org/10.1016/j.envres.2019.108550 |
| eu_rights_str_mv |
openAccess |
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application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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