Impact or performed T-cell alloreactivity by means of donor-specific and panel of reactive T-cells (PRT) Elispot in kidney transplantation

Donor-specific (d-sp) interferon gamma enzyme-linked immunosorbent spot (d-sp ELISPOT)and Panel of reactive T-cell (PRT) ELISPOT assays have been developed to detect alloreactive memory T (Tmem) cells in order to estimate the risk of acute rejection after kidney transplantation. Adding IL15 to the P...

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Autores: Gandolfini, Ilaria, Crespo, Elena, Baweja, Mukta, Jarque, Marta, Donadei, Chiara, Luque, Sergio, Montero Pérez, Núria, Allesina, Anna, Perin, Laura, Maggiore, Umberto, Cravedi, Paolo, Bestard Matamoros, Oriol
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/130524
Acceso en línea:https://hdl.handle.net/2445/130524
Access Level:acceso abierto
Palabra clave:Trasplantament renal
Immunologia de la trasplantació
Malalties del ronyó
Kidney transplantation
Transplantation immunology
Kidney diseases
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spelling Impact or performed T-cell alloreactivity by means of donor-specific and panel of reactive T-cells (PRT) Elispot in kidney transplantationGandolfini, IlariaCrespo, ElenaBaweja, MuktaJarque, MartaDonadei, ChiaraLuque, SergioMontero Pérez, NúriaAllesina, AnnaPerin, LauraMaggiore, UmbertoCravedi, PaoloBestard Matamoros, OriolTrasplantament renalImmunologia de la trasplantacióMalalties del ronyóKidney transplantationTransplantation immunologyKidney diseasesDonor-specific (d-sp) interferon gamma enzyme-linked immunosorbent spot (d-sp ELISPOT)and Panel of reactive T-cell (PRT) ELISPOT assays have been developed to detect alloreactive memory T (Tmem) cells in order to estimate the risk of acute rejection after kidney transplantation. Adding IL15 to the PRT assay (PRT+IL15) may uncover the presence of pathogenic alloreactive CD28-Tmem. Face-to-face comparisons of these assays have not been done yet. We performed pre-transplant d-sp ELISPOT and PRT assays (±IL15, against six B-cell lines) in 168 consecutive kidney transplant recipients and evaluated the multivariable-adjusted associations with biopsy-proven acute rejection (BPAR), de novo donor-specific antibodies (DSA), and eGFR decline over a 48-month follow-up period. D-sp ELISPOT was positive in 81 (48%) subjects, while 71 (42%) and 81 (48%) subjects displayed positive PRT and PRT+IL15, respectively.Their median [interquartile range] numerical test result was 23 [6±65], 18 [8±37], and 26 [10±45] spots/3x105 PBMCs, respectively. The number of PRT spots were weakly correlated with those of d-sp ELISPOT, but highly correlated with PRT+IL15 (rho = 0.96, P<0.001). d-sp ELISPOT, but not PRT (±IL15) was independently associated with BPAR (adjusted Odds Ratio of BPAR associated with d-sp ELISPOT positivity: 4.20 [95%CI: 1.06 to 21.73; P = 0.041]). Unlike d-sp ELISPOT, median PRT and PRT+IL15 were independently associated with higher Δ3-48month eGFR decline post-transplantation (for both assays, about -3mL/min/1.73m2 per one standard deviation unit increase in the spot number). Pre-transplant T-cell immune-monitoring using d-sp ELISPOT and PRT assays identifies kidney transplant candidates at high risk of BPAR and worse kidney allograft progression.Public Library of Science (PLoS)2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/130524Articles publicats en revistes (Ciències Clíniques)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1371/journal.pone.0200696PLoS One, 2018, vol. 13, num. 7, p. e200696https://doi.org/10.1371/journal.pone.0200696cc-by (c) Gandolfini, Ilaria et al., 2018http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1305242026-05-27T06:46:51Z
dc.title.none.fl_str_mv Impact or performed T-cell alloreactivity by means of donor-specific and panel of reactive T-cells (PRT) Elispot in kidney transplantation
title Impact or performed T-cell alloreactivity by means of donor-specific and panel of reactive T-cells (PRT) Elispot in kidney transplantation
spellingShingle Impact or performed T-cell alloreactivity by means of donor-specific and panel of reactive T-cells (PRT) Elispot in kidney transplantation
Gandolfini, Ilaria
Trasplantament renal
Immunologia de la trasplantació
Malalties del ronyó
Kidney transplantation
Transplantation immunology
Kidney diseases
title_short Impact or performed T-cell alloreactivity by means of donor-specific and panel of reactive T-cells (PRT) Elispot in kidney transplantation
title_full Impact or performed T-cell alloreactivity by means of donor-specific and panel of reactive T-cells (PRT) Elispot in kidney transplantation
title_fullStr Impact or performed T-cell alloreactivity by means of donor-specific and panel of reactive T-cells (PRT) Elispot in kidney transplantation
title_full_unstemmed Impact or performed T-cell alloreactivity by means of donor-specific and panel of reactive T-cells (PRT) Elispot in kidney transplantation
title_sort Impact or performed T-cell alloreactivity by means of donor-specific and panel of reactive T-cells (PRT) Elispot in kidney transplantation
dc.creator.none.fl_str_mv Gandolfini, Ilaria
Crespo, Elena
Baweja, Mukta
Jarque, Marta
Donadei, Chiara
Luque, Sergio
Montero Pérez, Núria
Allesina, Anna
Perin, Laura
Maggiore, Umberto
Cravedi, Paolo
Bestard Matamoros, Oriol
author Gandolfini, Ilaria
author_facet Gandolfini, Ilaria
Crespo, Elena
Baweja, Mukta
Jarque, Marta
Donadei, Chiara
Luque, Sergio
Montero Pérez, Núria
Allesina, Anna
Perin, Laura
Maggiore, Umberto
Cravedi, Paolo
Bestard Matamoros, Oriol
author_role author
author2 Crespo, Elena
Baweja, Mukta
Jarque, Marta
Donadei, Chiara
Luque, Sergio
Montero Pérez, Núria
Allesina, Anna
Perin, Laura
Maggiore, Umberto
Cravedi, Paolo
Bestard Matamoros, Oriol
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Trasplantament renal
Immunologia de la trasplantació
Malalties del ronyó
Kidney transplantation
Transplantation immunology
Kidney diseases
topic Trasplantament renal
Immunologia de la trasplantació
Malalties del ronyó
Kidney transplantation
Transplantation immunology
Kidney diseases
description Donor-specific (d-sp) interferon gamma enzyme-linked immunosorbent spot (d-sp ELISPOT)and Panel of reactive T-cell (PRT) ELISPOT assays have been developed to detect alloreactive memory T (Tmem) cells in order to estimate the risk of acute rejection after kidney transplantation. Adding IL15 to the PRT assay (PRT+IL15) may uncover the presence of pathogenic alloreactive CD28-Tmem. Face-to-face comparisons of these assays have not been done yet. We performed pre-transplant d-sp ELISPOT and PRT assays (±IL15, against six B-cell lines) in 168 consecutive kidney transplant recipients and evaluated the multivariable-adjusted associations with biopsy-proven acute rejection (BPAR), de novo donor-specific antibodies (DSA), and eGFR decline over a 48-month follow-up period. D-sp ELISPOT was positive in 81 (48%) subjects, while 71 (42%) and 81 (48%) subjects displayed positive PRT and PRT+IL15, respectively.Their median [interquartile range] numerical test result was 23 [6±65], 18 [8±37], and 26 [10±45] spots/3x105 PBMCs, respectively. The number of PRT spots were weakly correlated with those of d-sp ELISPOT, but highly correlated with PRT+IL15 (rho = 0.96, P<0.001). d-sp ELISPOT, but not PRT (±IL15) was independently associated with BPAR (adjusted Odds Ratio of BPAR associated with d-sp ELISPOT positivity: 4.20 [95%CI: 1.06 to 21.73; P = 0.041]). Unlike d-sp ELISPOT, median PRT and PRT+IL15 were independently associated with higher Δ3-48month eGFR decline post-transplantation (for both assays, about -3mL/min/1.73m2 per one standard deviation unit increase in the spot number). Pre-transplant T-cell immune-monitoring using d-sp ELISPOT and PRT assays identifies kidney transplant candidates at high risk of BPAR and worse kidney allograft progression.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/130524
url https://hdl.handle.net/2445/130524
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0200696
PLoS One, 2018, vol. 13, num. 7, p. e200696
https://doi.org/10.1371/journal.pone.0200696
dc.rights.none.fl_str_mv cc-by (c) Gandolfini, Ilaria et al., 2018
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Gandolfini, Ilaria et al., 2018
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Ciències Clíniques)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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