The therapeutic potential of fungal ribotoxins

Ribotoxins constitute a family of toxic extracellular fungal RNases that exert a highly specific activity on a conserved region of the larger molecule of rRNA, known as the sarcin–ricin loop. This cleavage of a single phosphodiester bond inactivates the ribosome and leads to protein synthesis inhibi...

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Authors: Carreras Sangrà, Nelson, Álvarez García, Elisa, Herrero Galán, Elías, Tomé, Jaime, Lacadena García-Gallo, Francisco Javier, Alegre Cebollada, Jorge, Oñaderra, Mercedes, Gavilanes, José G., Martínez Del Pozo, Álvaro
Format: article
Publication Date:2008
Country:España
Institution:Universidad Complutense de Madrid (UCM)
Repository:Docta Complutense
Language:English
OAI Identifier:oai:docta.ucm.es:20.500.14352/52957
Online Access:https://hdl.handle.net/20.500.14352/52957
Access Level:Open access
Keyword:Asp f 1
Fungal allergy
Immunotoxin
Restrictocin
RNase
Sarcin
Farmacología (Farmacia)
Medicamentos
Bioquímica (Biología)
Biotecnología
Biología molecular (Biología)
3209 Farmacología
2302 Bioquímica
3399 Otras Especialidades Tecnológicas
2415 Biología Molecular
id ES_19706b8a34e42f20cecc79ecf5d18aa2
oai_identifier_str oai:docta.ucm.es:20.500.14352/52957
network_acronym_str ES
network_name_str España
repository_id_str
spelling The therapeutic potential of fungal ribotoxinsCarreras Sangrà, NelsonÁlvarez García, ElisaHerrero Galán, ElíasTomé, JaimeLacadena García-Gallo, Francisco JavierAlegre Cebollada, JorgeOñaderra, MercedesGavilanes, José G.Martínez Del Pozo, ÁlvaroAsp f 1Fungal allergyImmunotoxinRestrictocinRNaseSarcinFarmacología (Farmacia)MedicamentosBioquímica (Biología)BiotecnologíaBiología molecular (Biología)3209 Farmacología2302 Bioquímica3399 Otras Especialidades Tecnológicas2415 Biología MolecularRibotoxins constitute a family of toxic extracellular fungal RNases that exert a highly specific activity on a conserved region of the larger molecule of rRNA, known as the sarcin–ricin loop. This cleavage of a single phosphodiester bond inactivates the ribosome and leads to protein synthesis inhibition and cell death. In addition to this ribonucleolytic activity, ribotoxins can cross lipid membranes in the absence of any known protein receptor. This ability is due to their capacity to interact with acid phospholipid-containing membranes. Both activities together explain their cytotoxic character, being rather specific when assayed against some transformed cell lines. The determination of high-resolution structures of some ribotoxins, the characterization of a large number of mutants, and the use of lipid model vesicles and transformed cell lines have been the tools used for the study of their mechanism of action at the molecular level. The present knowledge suggests that wild-type ribotoxins or some modified variants might be used in human therapies. Production of hypoallergenic mutants and immunotoxins designed against specific tumors stand out as feasible alternatives to treat some human pathology in the mid-term future.Bentham Science Publishers Ltd.Universidad Complutense de Madrid20082008-01-0120082008-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/52957reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/529572026-06-02T12:44:21Z
dc.title.none.fl_str_mv The therapeutic potential of fungal ribotoxins
title The therapeutic potential of fungal ribotoxins
spellingShingle The therapeutic potential of fungal ribotoxins
Carreras Sangrà, Nelson
Asp f 1
Fungal allergy
Immunotoxin
Restrictocin
RNase
Sarcin
Farmacología (Farmacia)
Medicamentos
Bioquímica (Biología)
Biotecnología
Biología molecular (Biología)
3209 Farmacología
2302 Bioquímica
3399 Otras Especialidades Tecnológicas
2415 Biología Molecular
title_short The therapeutic potential of fungal ribotoxins
title_full The therapeutic potential of fungal ribotoxins
title_fullStr The therapeutic potential of fungal ribotoxins
title_full_unstemmed The therapeutic potential of fungal ribotoxins
title_sort The therapeutic potential of fungal ribotoxins
dc.creator.none.fl_str_mv Carreras Sangrà, Nelson
Álvarez García, Elisa
Herrero Galán, Elías
Tomé, Jaime
Lacadena García-Gallo, Francisco Javier
Alegre Cebollada, Jorge
Oñaderra, Mercedes
Gavilanes, José G.
Martínez Del Pozo, Álvaro
author Carreras Sangrà, Nelson
author_facet Carreras Sangrà, Nelson
Álvarez García, Elisa
Herrero Galán, Elías
Tomé, Jaime
Lacadena García-Gallo, Francisco Javier
Alegre Cebollada, Jorge
Oñaderra, Mercedes
Gavilanes, José G.
Martínez Del Pozo, Álvaro
author_role author
author2 Álvarez García, Elisa
Herrero Galán, Elías
Tomé, Jaime
Lacadena García-Gallo, Francisco Javier
Alegre Cebollada, Jorge
Oñaderra, Mercedes
Gavilanes, José G.
Martínez Del Pozo, Álvaro
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Asp f 1
Fungal allergy
Immunotoxin
Restrictocin
RNase
Sarcin
Farmacología (Farmacia)
Medicamentos
Bioquímica (Biología)
Biotecnología
Biología molecular (Biología)
3209 Farmacología
2302 Bioquímica
3399 Otras Especialidades Tecnológicas
2415 Biología Molecular
topic Asp f 1
Fungal allergy
Immunotoxin
Restrictocin
RNase
Sarcin
Farmacología (Farmacia)
Medicamentos
Bioquímica (Biología)
Biotecnología
Biología molecular (Biología)
3209 Farmacología
2302 Bioquímica
3399 Otras Especialidades Tecnológicas
2415 Biología Molecular
description Ribotoxins constitute a family of toxic extracellular fungal RNases that exert a highly specific activity on a conserved region of the larger molecule of rRNA, known as the sarcin–ricin loop. This cleavage of a single phosphodiester bond inactivates the ribosome and leads to protein synthesis inhibition and cell death. In addition to this ribonucleolytic activity, ribotoxins can cross lipid membranes in the absence of any known protein receptor. This ability is due to their capacity to interact with acid phospholipid-containing membranes. Both activities together explain their cytotoxic character, being rather specific when assayed against some transformed cell lines. The determination of high-resolution structures of some ribotoxins, the characterization of a large number of mutants, and the use of lipid model vesicles and transformed cell lines have been the tools used for the study of their mechanism of action at the molecular level. The present knowledge suggests that wild-type ribotoxins or some modified variants might be used in human therapies. Production of hypoallergenic mutants and immunotoxins designed against specific tumors stand out as feasible alternatives to treat some human pathology in the mid-term future.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01
2008
2008-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/52957
url https://hdl.handle.net/20.500.14352/52957
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Bentham Science Publishers Ltd.
publisher.none.fl_str_mv Bentham Science Publishers Ltd.
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869404046405664768
score 15,301603

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