An immobilized and highly stabilized self-sufficientmonooxygenase as biocatalyst for oxidative biotransformations
Background: the requirement for expensive cofactors that must be efficiently recycled is one of the major factors hindering the wide implementation of industrial biocatalytic oxidation processes. In this research, a sustainable approach based on immobilized self-sufficient Baeyer-Villiger monooxygen...
| Autores: | , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2018 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:196152 |
| Acceso en línea: | https://ddd.uab.cat/record/196152 https://dx.doi.org/urn:doi:10.1002/jctb.5450 |
| Access Level: | acceso abierto |
| Palabra clave: | Cyclohexanone monooxygenase (CHMO) Phosphite dehydrogenase (PTDH) Multi-enzymatic reactions Biocatalyst immobilization Biocatalyst reutilization Cofactor recycling |
| Sumario: | Background: the requirement for expensive cofactors that must be efficiently recycled is one of the major factors hindering the wide implementation of industrial biocatalytic oxidation processes. In this research, a sustainable approach based on immobilized self-sufficient Baeyer-Villiger monooxygenases is discussed. - Results: a bifunctional biocatalyst composed of an NADPH-dependent cyclohexanone monooxygenase (CHMO) fused to an NADP+-accepting phosphite dehydrogenase (PTDH) catalyzes ϵ-caprolactone synthesis from cyclohexanone, using phosphite as a cheap sacrificial substrate for cofactor regeneration. Several immobilized derivatives of the fused enzyme have been prepared with high immobilization yield (97%); the one obtained by affinity adsorption on Co-IDA (Co: cobalt chelated; IDA: iminodiacetic acid) support has shown to be highly stable affording average yields of 80% after 18 reaction cycles. - Conclusions -the immobilized self-sufficient monooxygenase has demonstrated to be able to perform Baeyer-Villiger oxidation with efficient cofactor recovery and biocatalyst recycling. The proposed biocatalytic process offers access to valuable molecules with high atom economy and limited waste generation. |
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