qPCR as a powerful tool for microbial food spoilage quantification: significance for food quality

The use of real time quantitative PCR (qPCR) has recently been extended to food science. The literature has mainly focused on its use in ensuring food safety. However, it offers a number of advantages with respect to the quantification of non-pathogenic food spoilage microorganisms. Indeed, qPCR may...

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Detalles Bibliográficos
Autores: Martínez Álvarez, Noelia, Martín, María Cruz, Fresno Herrero, Ana, Fernández García, María, Álvarez González, Miguel Ángel, Ladero Losada, Víctor
Tipo de recurso: artículo
Fecha de publicación:2011
País:España
Institución:Universidad de Santiago de Compostela (USC)
Repositorio:Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela
Idioma:inglés
OAI Identifier:oai:minerva.usc.gal:10347/45528
Acceso en línea:https://hdl.handle.net/10347/45528
Access Level:acceso abierto
Palabra clave:qPCR
Food
Quality
Microorganisms
Spoilage
Microbial quantification
3309 Tecnología de los alimentos
2414 Microbiología
Descripción
Sumario:The use of real time quantitative PCR (qPCR) has recently been extended to food science. The literature has mainly focused on its use in ensuring food safety. However, it offers a number of advantages with respect to the quantification of non-pathogenic food spoilage microorganisms. Indeed, qPCR may have a promising future in improving the quality of food products. The present review examines the use of qPCR in this area, the basis of the technique, the requirements that must be met for optimal qPCR assays to be performed, and the advantages it offers over other techniques