Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensor

Ostreopsis cf. ovata is a benthic microalga distributed in tropical and temperate regions worldwide which produces palytoxins (PlTXs). Herein, an electrochemical biosensor for the detection of this toxic microalga is described. The detection strategy involves isothermal recombinase polymerase amplif...

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Detalles Bibliográficos
Autores: Toldrà, Anna, Alcaraz, Carles, Diogène, Jorge, O'Sullivan, Ciara K., Campàs, Mònica
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/510
Acceso en línea:http://hdl.handle.net/20.500.12327/510
https://doi.org/10.1016/j.scitotenv.2019.06.448
Access Level:acceso abierto
Palabra clave:63
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spelling Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensorToldrà, AnnaAlcaraz, CarlesDiogène, JorgeO'Sullivan, Ciara K.Campàs, Mònica63Ostreopsis cf. ovata is a benthic microalga distributed in tropical and temperate regions worldwide which produces palytoxins (PlTXs). Herein, an electrochemical biosensor for the detection of this toxic microalga is described. The detection strategy involves isothermal recombinase polymerase amplification (RPA) of the target using tailed primers and a sandwich hybridisation assay on maleimide-coated magnetic beads immobilised on electrode arrays. The biosensor attained a limit of detection of 9 pg/μL of O. cf. ovata DNA (which corresponds to ~640 cells/L), with no interferences from two non-target Ostreopsis species (O. cf. siamensis and O. fattorussoi). The biosensor was applied to the analysis of planktonic and benthic environmental samples. Electrochemical O. cf. ovata DNA quantifications demonstrated an excellent correlation with other molecular methods (qPCR and colorimetric assays) and allowed the construction of a predictive regression model to estimate O. cf. ovata cell abundances. This new technology offer great potential to improve research, monitoring and management of O. cf. ovata and harmful algal blooms.info:eu-repo/semantics/acceptedVersionElsevierProducció AnimalAigües Marines i Continentals2019info:eu-repo/semantics/article26http://hdl.handle.net/20.500.12327/510https://doi.org/10.1016/j.scitotenv.2019.06.448reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésScience of the Total EnvironmentMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2014-56024-C2-2-R/ES/Microsistemas para la detección rapida, fiable y rentable de microalgas toxicas in situ y a tiempo real/SEASENSINGMINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2017-87946-C2-2-R/ES/Dispositivos bioanaliticos para la detección rapida y rentable de gambierdiscus y ciguatoxinas/CIGUASENSINGAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/5102026-05-29T05:05:01Z
dc.title.none.fl_str_mv Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensor
title Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensor
spellingShingle Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensor
Toldrà, Anna
63
title_short Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensor
title_full Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensor
title_fullStr Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensor
title_full_unstemmed Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensor
title_sort Detection of Ostreopsis cf. ovata in environmental samples using an electrochemical DNA-based biosensor
dc.creator.none.fl_str_mv Toldrà, Anna
Alcaraz, Carles
Diogène, Jorge
O'Sullivan, Ciara K.
Campàs, Mònica
author Toldrà, Anna
author_facet Toldrà, Anna
Alcaraz, Carles
Diogène, Jorge
O'Sullivan, Ciara K.
Campàs, Mònica
author_role author
author2 Alcaraz, Carles
Diogène, Jorge
O'Sullivan, Ciara K.
Campàs, Mònica
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Aigües Marines i Continentals
dc.subject.none.fl_str_mv 63
topic 63
description Ostreopsis cf. ovata is a benthic microalga distributed in tropical and temperate regions worldwide which produces palytoxins (PlTXs). Herein, an electrochemical biosensor for the detection of this toxic microalga is described. The detection strategy involves isothermal recombinase polymerase amplification (RPA) of the target using tailed primers and a sandwich hybridisation assay on maleimide-coated magnetic beads immobilised on electrode arrays. The biosensor attained a limit of detection of 9 pg/μL of O. cf. ovata DNA (which corresponds to ~640 cells/L), with no interferences from two non-target Ostreopsis species (O. cf. siamensis and O. fattorussoi). The biosensor was applied to the analysis of planktonic and benthic environmental samples. Electrochemical O. cf. ovata DNA quantifications demonstrated an excellent correlation with other molecular methods (qPCR and colorimetric assays) and allowed the construction of a predictive regression model to estimate O. cf. ovata cell abundances. This new technology offer great potential to improve research, monitoring and management of O. cf. ovata and harmful algal blooms.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/510
https://doi.org/10.1016/j.scitotenv.2019.06.448
url http://hdl.handle.net/20.500.12327/510
https://doi.org/10.1016/j.scitotenv.2019.06.448
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Science of the Total Environment
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2014-56024-C2-2-R/ES/Microsistemas para la detección rapida, fiable y rentable de microalgas toxicas in situ y a tiempo real/SEASENSING
MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/BIO2017-87946-C2-2-R/ES/Dispositivos bioanaliticos para la detección rapida y rentable de gambierdiscus y ciguatoxinas/CIGUASENSING
dc.rights.none.fl_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 26
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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