In vitro digestibility of dietary proteins and in vitro DIAAS analytical workflow based on the INFOGEST static protocol and its validation with in vivo data

The FAO recommends the digestible indispensable amino acid score (DIAAS) to determine protein quality in foods, preferably tested in vivo. Here, the INFOGEST in vitro digestion protocol was applied and supplemented with an analytical workflow allowing the assessment of protein digestibility and DIAA...

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Detalles Bibliográficos
Autores: Sousa, Raquel, Recio, Isidra, Heimo, Dominique, Dubois, Sébastien, Moughan, Paul J., Hodgkinson, Suzanne M., Portmann, Reto, Egger, Lotti
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/309554
Acceso en línea:http://hdl.handle.net/10261/309554
Access Level:acceso abierto
Palabra clave:Digestibility
In vitro DIAAS
Total amino acids
In vitro digestion
Liquid chromatography–mass spectrometry
Protein hydrolysis
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spelling In vitro digestibility of dietary proteins and in vitro DIAAS analytical workflow based on the INFOGEST static protocol and its validation with in vivo dataSousa, RaquelRecio, IsidraHeimo, DominiqueDubois, SébastienMoughan, Paul J.Hodgkinson, Suzanne M.Portmann, RetoEgger, LottiDigestibilityIn vitro DIAASTotal amino acidsIn vitro digestionLiquid chromatography–mass spectrometryProtein hydrolysisThe FAO recommends the digestible indispensable amino acid score (DIAAS) to determine protein quality in foods, preferably tested in vivo. Here, the INFOGEST in vitro digestion protocol was applied and supplemented with an analytical workflow allowing the assessment of protein digestibility and DIAAS calculation. The protocol was applied to selected samples WPI, zein, collagen, black beans, pigeon peas, All-Bran®, and peanuts. The total protein digestibility, digestibility of individual amino acids (AA), and DIAAS values were established and compared with in vivo data for the same substrates. Total protein digestibility (total Nitrogen, r = 0.7, P < 0.05; primary amines (OPA), r = 0.6, P < 0.02; total AA, r = 0.6, P < 0.02) and digestibility of individual AA (r = 0.6, P < 0.0001) were in good agreement, between in vitro and in vivo, with a mean difference of 1.2 %. In vitro DIAAS was highly correlated with DIAAS obtained from in vivo true ileal digestibility values (r = 0.96, R2 = 0.89, P < 0.0001) with a mean difference of 0.1 %.IR acknowledges the project PID2019-107663RB-I00 funded by MCIN/AEI/10.13039/501100011033.Peer reviewedElsevierMinisterio de Ciencia, Innovación y Universidades (España)Ministerio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/309554reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107663RB-I00Food Chemistryhttps://doi.org/10.1016/j.foodchem.2022.134720Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3095542026-05-22T06:33:51Z
dc.title.none.fl_str_mv In vitro digestibility of dietary proteins and in vitro DIAAS analytical workflow based on the INFOGEST static protocol and its validation with in vivo data
title In vitro digestibility of dietary proteins and in vitro DIAAS analytical workflow based on the INFOGEST static protocol and its validation with in vivo data
spellingShingle In vitro digestibility of dietary proteins and in vitro DIAAS analytical workflow based on the INFOGEST static protocol and its validation with in vivo data
Sousa, Raquel
Digestibility
In vitro DIAAS
Total amino acids
In vitro digestion
Liquid chromatography–mass spectrometry
Protein hydrolysis
title_short In vitro digestibility of dietary proteins and in vitro DIAAS analytical workflow based on the INFOGEST static protocol and its validation with in vivo data
title_full In vitro digestibility of dietary proteins and in vitro DIAAS analytical workflow based on the INFOGEST static protocol and its validation with in vivo data
title_fullStr In vitro digestibility of dietary proteins and in vitro DIAAS analytical workflow based on the INFOGEST static protocol and its validation with in vivo data
title_full_unstemmed In vitro digestibility of dietary proteins and in vitro DIAAS analytical workflow based on the INFOGEST static protocol and its validation with in vivo data
title_sort In vitro digestibility of dietary proteins and in vitro DIAAS analytical workflow based on the INFOGEST static protocol and its validation with in vivo data
dc.creator.none.fl_str_mv Sousa, Raquel
Recio, Isidra
Heimo, Dominique
Dubois, Sébastien
Moughan, Paul J.
Hodgkinson, Suzanne M.
Portmann, Reto
Egger, Lotti
author Sousa, Raquel
author_facet Sousa, Raquel
Recio, Isidra
Heimo, Dominique
Dubois, Sébastien
Moughan, Paul J.
Hodgkinson, Suzanne M.
Portmann, Reto
Egger, Lotti
author_role author
author2 Recio, Isidra
Heimo, Dominique
Dubois, Sébastien
Moughan, Paul J.
Hodgkinson, Suzanne M.
Portmann, Reto
Egger, Lotti
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Digestibility
In vitro DIAAS
Total amino acids
In vitro digestion
Liquid chromatography–mass spectrometry
Protein hydrolysis
topic Digestibility
In vitro DIAAS
Total amino acids
In vitro digestion
Liquid chromatography–mass spectrometry
Protein hydrolysis
description The FAO recommends the digestible indispensable amino acid score (DIAAS) to determine protein quality in foods, preferably tested in vivo. Here, the INFOGEST in vitro digestion protocol was applied and supplemented with an analytical workflow allowing the assessment of protein digestibility and DIAAS calculation. The protocol was applied to selected samples WPI, zein, collagen, black beans, pigeon peas, All-Bran®, and peanuts. The total protein digestibility, digestibility of individual amino acids (AA), and DIAAS values were established and compared with in vivo data for the same substrates. Total protein digestibility (total Nitrogen, r = 0.7, P < 0.05; primary amines (OPA), r = 0.6, P < 0.02; total AA, r = 0.6, P < 0.02) and digestibility of individual AA (r = 0.6, P < 0.0001) were in good agreement, between in vitro and in vivo, with a mean difference of 1.2 %. In vitro DIAAS was highly correlated with DIAAS obtained from in vivo true ileal digestibility values (r = 0.96, R2 = 0.89, P < 0.0001) with a mean difference of 0.1 %.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/309554
url http://hdl.handle.net/10261/309554
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107663RB-I00
Food Chemistry
https://doi.org/10.1016/j.foodchem.2022.134720
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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