In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer Cells

Colorectal cancer is the third most common cancer in the world. Many efforts have focused on finding natural molecules with potential chemo-preventive activity due to their low toxicity compared to synthetic drugs. However, comprehensive information on the bioactive fractions and components is still...

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Detalles Bibliográficos
Autores: Jaramillo Carmona, Sara, Guillén Bejarano, Rafael, Jiménez Araujo, Ana José, Rodríguez Arcos, Rocío, López Martín, Sergio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/130542
Acceso en línea:https://hdl.handle.net/11441/130542
https://doi.org/10.1002/cbdv.201800282
Access Level:acceso abierto
Palabra clave:Asparagus
Biological activity
Colon cancer cells
Cytotoxicity
Saponins
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spelling In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer CellsJaramillo Carmona, SaraGuillén Bejarano, RafaelJiménez Araujo, Ana JoséRodríguez Arcos, RocíoLópez Martín, SergioAsparagusBiological activityColon cancer cellsCytotoxicitySaponinsColorectal cancer is the third most common cancer in the world. Many efforts have focused on finding natural molecules with potential chemo-preventive activity due to their low toxicity compared to synthetic drugs. However, comprehensive information on the bioactive fractions and components is still missing. In this study, we developed a method for the quantitative separation and isolation of saponins from asparagus genotypes consisting of an adsorption chromatography and subsequent liquid chromatographic separation on a reversed-phase column. The saponins isolated were tested for their cytotoxic activity against human colon cancer cell lines, which could develop cross-resistance to a wide variety of chemotherapeutic drugs. Our results showed that Huétor-Tájar asparagus saponins (HTSAP), mainly protodioscin and HTSAP-10 have higher cytotoxic activity than HTSAP-1, HTSAP-6, and HTSAP-8. This study links the potential anticancer effect of asparagus to specific saponins and unveils the triguero Huétor-Tájar asparagus as a nutraceutical particularly in colon cancer therapiesMinisterio de Economía y Competitividad JCI-2012-13084Wiley-BlackwellBiología Celular2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/130542https://doi.org/10.1002/cbdv.201800282reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésChemistry and Biodiversity, 15 (11), e1800282.JCI-2012-13084https://doi.org/10.1002/cbdv.201800282info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1305422026-06-17T12:51:07Z
dc.title.none.fl_str_mv In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer Cells
title In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer Cells
spellingShingle In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer Cells
Jaramillo Carmona, Sara
Asparagus
Biological activity
Colon cancer cells
Cytotoxicity
Saponins
title_short In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer Cells
title_full In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer Cells
title_fullStr In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer Cells
title_full_unstemmed In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer Cells
title_sort In Vitro Toxicity of Asparagus Saponins in Distinct Multidrug-Resistant Colon Cancer Cells
dc.creator.none.fl_str_mv Jaramillo Carmona, Sara
Guillén Bejarano, Rafael
Jiménez Araujo, Ana José
Rodríguez Arcos, Rocío
López Martín, Sergio
author Jaramillo Carmona, Sara
author_facet Jaramillo Carmona, Sara
Guillén Bejarano, Rafael
Jiménez Araujo, Ana José
Rodríguez Arcos, Rocío
López Martín, Sergio
author_role author
author2 Guillén Bejarano, Rafael
Jiménez Araujo, Ana José
Rodríguez Arcos, Rocío
López Martín, Sergio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Biología Celular
dc.subject.none.fl_str_mv Asparagus
Biological activity
Colon cancer cells
Cytotoxicity
Saponins
topic Asparagus
Biological activity
Colon cancer cells
Cytotoxicity
Saponins
description Colorectal cancer is the third most common cancer in the world. Many efforts have focused on finding natural molecules with potential chemo-preventive activity due to their low toxicity compared to synthetic drugs. However, comprehensive information on the bioactive fractions and components is still missing. In this study, we developed a method for the quantitative separation and isolation of saponins from asparagus genotypes consisting of an adsorption chromatography and subsequent liquid chromatographic separation on a reversed-phase column. The saponins isolated were tested for their cytotoxic activity against human colon cancer cell lines, which could develop cross-resistance to a wide variety of chemotherapeutic drugs. Our results showed that Huétor-Tájar asparagus saponins (HTSAP), mainly protodioscin and HTSAP-10 have higher cytotoxic activity than HTSAP-1, HTSAP-6, and HTSAP-8. This study links the potential anticancer effect of asparagus to specific saponins and unveils the triguero Huétor-Tájar asparagus as a nutraceutical particularly in colon cancer therapies
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/130542
https://doi.org/10.1002/cbdv.201800282
url https://hdl.handle.net/11441/130542
https://doi.org/10.1002/cbdv.201800282
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Chemistry and Biodiversity, 15 (11), e1800282.
JCI-2012-13084
https://doi.org/10.1002/cbdv.201800282
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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