Immunophenotypic Analysis of Acute Megakaryoblastic Leukemia: A EuroFlow Study
Acute megakaryoblastic leukemia (AMKL) is a rare and heterogeneous subtype of acute myeloid leukemia (AML). We evaluated the immunophenotypic profile of 72 AMKL and 114 non-AMKL AML patients using the EuroFlow AML panel. Univariate and multivariate/multidimensional analyses were performed to identif...
| Autores: | , , , , , , , , , , , , , , , |
|---|---|
| Formato: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2022 |
| País: | España |
| Recursos: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/282906 |
| Acesso em linha: | http://hdl.handle.net/10261/282906 |
| Access Level: | acceso abierto |
| Palavra-chave: | EuroFlow Immunophenotyping Down syndrome Transient abnormal myelopoiesis AMKL |
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Immunophenotypic Analysis of Acute Megakaryoblastic Leukemia: A EuroFlow StudyBrouwer, NienkeMatarraz, SergioNierkens, StefanHofmans, MattiasNovákova, MichaelaSobral da Costa, E.Fernández, PaulaBras, A. E.Vieira de Mello, FabianaMejstrikova, EsterPhilippé, J.Grigore, Georgiana EmiliaPedreira, C. E.Dongen, J. J. M. vanOrfao, AlbertoVelden, Vincent H. J. van derEuroFlowImmunophenotypingDown syndromeTransient abnormal myelopoiesisAMKLAcute megakaryoblastic leukemia (AMKL) is a rare and heterogeneous subtype of acute myeloid leukemia (AML). We evaluated the immunophenotypic profile of 72 AMKL and 114 non-AMKL AML patients using the EuroFlow AML panel. Univariate and multivariate/multidimensional analyses were performed to identify most relevant markers contributing to the diagnosis of AMKL. AMKL patients were subdivided into transient abnormal myelopoiesis (TAM), myeloid leukemia associated with Down syndrome (ML-DS), AML—not otherwise specified with megakaryocytic differentiation (NOS-AMKL), and AMKL—other patients (AML patients with other WHO classification but with flowcytometric features of megakaryocytic differentiation). Flowcytometric analysis showed good discrimination between AMKL and non-AMKL patients based on differential expression of, in particular, CD42a.CD61, CD41, CD42b, HLADR, CD15 and CD13. Combining CD42a.CD61 (positive) and CD13 (negative) resulted in a sensitivity of 71% and a specificity of 99%. Within AMKL patients, TAM and ML-DS patients showed higher frequencies of immature CD34+/CD117+ leukemic cells as compared to NOS-AMKL and AMKL-Other patients. In addition, ML-DS patients showed a significantly higher expression of CD33, CD11b, CD38 and CD7 as compared to the other three subgroups, allowing for good distinction of these patients. Overall, our data show that the EuroFlow AML panel allows for straightforward diagnosis of AMKL and that ML-DS is associated with a unique immunophenotypic profile.The EuroFlow Consortium received support from the FP6-2004-LIFESCIHEALTH-5 program of the European Commission (grant LSHB-CT-2006-018708) as Specific Targeted Research Project (STREP). The EuroFlow Consortium is part of the European Scientific Foundation for HematoOncology (ESLHO), a Scientific Working Group (SWG) of the European Hematology Association (EHA). The work of C.E.P. and E.S.C. was partially supported by FAPERJ (Grant E26/200.840/2021- CNE; E26/210.379/2018 and E26/110.105/2014); CAPES-PROEX; and CNPq (Grant 306258/2019-6 and 303765/2018-6). M.N. and E.M. were supported by the Ministry of Health of the Czech Republic, grant number NU20J-07-00028. S.M. was supported by Acción Estratégica en Salud (AES) (Grant PI21_01115) and the grant of CIBERONC of the Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación, Madrid, Spain and FONDOS FEDER (no. CB16/12/00400).Multidisciplinary Digital Publishing InstituteEuropean CommissionEuropean Hematology AssociationConselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)Ministry of Health of the Czech RepublicInstituto de Salud Carlos IIIMinisterio de Ciencia e Innovación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/282906reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/cancers14061583http://dx.doi.org/10.3390/cancers14061583Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2829062026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
Immunophenotypic Analysis of Acute Megakaryoblastic Leukemia: A EuroFlow Study |
| title |
Immunophenotypic Analysis of Acute Megakaryoblastic Leukemia: A EuroFlow Study |
| spellingShingle |
Immunophenotypic Analysis of Acute Megakaryoblastic Leukemia: A EuroFlow Study Brouwer, Nienke EuroFlow Immunophenotyping Down syndrome Transient abnormal myelopoiesis AMKL |
| title_short |
Immunophenotypic Analysis of Acute Megakaryoblastic Leukemia: A EuroFlow Study |
| title_full |
Immunophenotypic Analysis of Acute Megakaryoblastic Leukemia: A EuroFlow Study |
| title_fullStr |
Immunophenotypic Analysis of Acute Megakaryoblastic Leukemia: A EuroFlow Study |
| title_full_unstemmed |
Immunophenotypic Analysis of Acute Megakaryoblastic Leukemia: A EuroFlow Study |
| title_sort |
Immunophenotypic Analysis of Acute Megakaryoblastic Leukemia: A EuroFlow Study |
| dc.creator.none.fl_str_mv |
Brouwer, Nienke Matarraz, Sergio Nierkens, Stefan Hofmans, Mattias Novákova, Michaela Sobral da Costa, E. Fernández, Paula Bras, A. E. Vieira de Mello, Fabiana Mejstrikova, Ester Philippé, J. Grigore, Georgiana Emilia Pedreira, C. E. Dongen, J. J. M. van Orfao, Alberto Velden, Vincent H. J. van der |
| author |
Brouwer, Nienke |
| author_facet |
Brouwer, Nienke Matarraz, Sergio Nierkens, Stefan Hofmans, Mattias Novákova, Michaela Sobral da Costa, E. Fernández, Paula Bras, A. E. Vieira de Mello, Fabiana Mejstrikova, Ester Philippé, J. Grigore, Georgiana Emilia Pedreira, C. E. Dongen, J. J. M. van Orfao, Alberto Velden, Vincent H. J. van der |
| author_role |
author |
| author2 |
Matarraz, Sergio Nierkens, Stefan Hofmans, Mattias Novákova, Michaela Sobral da Costa, E. Fernández, Paula Bras, A. E. Vieira de Mello, Fabiana Mejstrikova, Ester Philippé, J. Grigore, Georgiana Emilia Pedreira, C. E. Dongen, J. J. M. van Orfao, Alberto Velden, Vincent H. J. van der |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
European Commission European Hematology Association Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil) Ministry of Health of the Czech Republic Instituto de Salud Carlos III Ministerio de Ciencia e Innovación (España) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
EuroFlow Immunophenotyping Down syndrome Transient abnormal myelopoiesis AMKL |
| topic |
EuroFlow Immunophenotyping Down syndrome Transient abnormal myelopoiesis AMKL |
| description |
Acute megakaryoblastic leukemia (AMKL) is a rare and heterogeneous subtype of acute myeloid leukemia (AML). We evaluated the immunophenotypic profile of 72 AMKL and 114 non-AMKL AML patients using the EuroFlow AML panel. Univariate and multivariate/multidimensional analyses were performed to identify most relevant markers contributing to the diagnosis of AMKL. AMKL patients were subdivided into transient abnormal myelopoiesis (TAM), myeloid leukemia associated with Down syndrome (ML-DS), AML—not otherwise specified with megakaryocytic differentiation (NOS-AMKL), and AMKL—other patients (AML patients with other WHO classification but with flowcytometric features of megakaryocytic differentiation). Flowcytometric analysis showed good discrimination between AMKL and non-AMKL patients based on differential expression of, in particular, CD42a.CD61, CD41, CD42b, HLADR, CD15 and CD13. Combining CD42a.CD61 (positive) and CD13 (negative) resulted in a sensitivity of 71% and a specificity of 99%. Within AMKL patients, TAM and ML-DS patients showed higher frequencies of immature CD34+/CD117+ leukemic cells as compared to NOS-AMKL and AMKL-Other patients. In addition, ML-DS patients showed a significantly higher expression of CD33, CD11b, CD38 and CD7 as compared to the other three subgroups, allowing for good distinction of these patients. Overall, our data show that the EuroFlow AML panel allows for straightforward diagnosis of AMKL and that ML-DS is associated with a unique immunophenotypic profile. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2022 2022 2022 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/282906 |
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http://hdl.handle.net/10261/282906 |
| dc.language.none.fl_str_mv |
Inglés |
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Inglés |
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The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI 10.3390/cancers14061583 http://dx.doi.org/10.3390/cancers14061583 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Multidisciplinary Digital Publishing Institute |
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Multidisciplinary Digital Publishing Institute |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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