Association of circulating microRNAs with coronary artery disease and usefulness for reclassification of healthy individuals

Risk prediction tools cannot identify most individuals at high coronary artery disease (CAD) risk. Oxidized low-density lipoproteins (oxLDLs) and microRNAs are actively involved in atherosclerosis. Our aim was to examine the association of CAD and oxLDLs-induced microRNAs, and to assess the microRNA...

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Detalhes bibliográficos
Autores: Degano, Irene R.|||0000-0003-1914-6934, Camps-Vilaró, Anna|||0000-0001-9644-8421, Subirana, Isaac|||0000-0003-1676-0197, García-Mateo, Nadia, Cidad, Pilar, Muñoz Aguayo, Daniel|||0000-0001-8173-0168, Puigdecanet, Eulàlia|||0000-0001-6067-9332, Nonell, Lara|||0000-0002-8538-8213, Vila-Domènech, Joan Salvador|||0000-0002-0413-9291, Crepaldi, Felipe Mingorance|||0000-0002-8500-1162, Gonzalo Calvo, David de|||0000-0003-2240-3532, Llorente-Cortés, Vicenta|||0000-0002-0067-7201, Pérez-García, María Teresa|||0000-0001-8540-8117, Elosua, Roberto|||0000-0001-8235-0095, Fitó, Montserrat|||0000-0002-1817-483X, Marrugat, Jaume|||0000-0003-3320-554X
Tipo de documento: artigo
Data de publicação:2020
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:283557
Acesso em linha:https://ddd.uab.cat/record/283557
https://dx.doi.org/urn:doi:10.3390/jcm9051402
Access Level:Acceso aberto
Palavra-chave:Circulating biomarkers
Coronary artery disease
MicroRNAs
Myocardial infarction
Oxidized low-density lipoproteins
Descrição
Resumo:Risk prediction tools cannot identify most individuals at high coronary artery disease (CAD) risk. Oxidized low-density lipoproteins (oxLDLs) and microRNAs are actively involved in atherosclerosis. Our aim was to examine the association of CAD and oxLDLs-induced microRNAs, and to assess the microRNAs predictive capacity of future CAD events. Human endothelial and vascular smooth muscle cells were treated with oxidized/native low-density lipoproteins, and microRNA expression was analyzed. Differentially expressed and CAD-related miRNAs were examined in serum samples from (1) a case-control study with 476 myocardial infarction (MI) patients and 487 controls, and (2) a case-cohort study with 105 incident CAD cases and 455 randomly-selected cohort participants. MicroRNA expression was analyzed with custom OpenArray plates, log rank tests and Cox regression models. Twenty-one microRNAs, two previously undescribed (hsa-miR-193b-5p and hsa-miR-1229-5p), were up- or down-regulated upon cell treatment with oxLDLs. One of the 21, hsa-miR-122-5p, was also upregulated in MI cases (fold change = 4.85). Of the 28 CAD-related microRNAs tested, 11 were upregulated in MI cases -1 previously undescribed (hsa-miR-16-5p)-, and 1/11 was also associated with CAD incidence (adjusted hazard ratio = 0.55 (0.35-0.88)) and improved CAD risk reclassification, hsa-miR-143-3p. We identified 2 novel microRNAs modulated by oxLDLs in endothelial cells, 1 novel microRNA upregulated in AMI cases compared to controls, and one circulating microRNA that improved CAD risk classification.

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