Immunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25

Small ruminant lentiviruses (SRLV) are responsible for significant economic losses in sheep and goat farming; however, effective vaccination strategies remain unavailable. This study evaluated the immunogenicity, safety, and protective efficacy of a recombinant Sendai virus vector (SeV) expressing S...

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Autores: Gómez, Álex, Reina, R., Glaría Ezquer, Idoia, Moncayola, Irati, Echeverría Garín, Irache, Rodríguez Largo, Ana, Blas, Ignacio de, Pérez, Estela, Pérez, Marta María, Villanueva-Saz, Sergio, Lee, Benhur, Diego, Alicia de, Miguel, Ricardo de, Luján, Lluís
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/56067
Acceso en línea:https://hdl.handle.net/2454/56067
Access Level:acceso abierto
Palabra clave:Intranasal
Sendai
Small ruminant lentiviruses
Vaccine
Viral vector
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spelling Immunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25Gómez, ÁlexReina, R.Glaría Ezquer, IdoiaMoncayola, IratiEcheverría Garín, IracheRodríguez Largo, Ana Blas, Ignacio dePérez, EstelaPérez, Marta MaríaVillanueva-Saz, SergioLee, BenhurDiego, Alicia deMiguel, Ricardo deLuján, LluísIntranasalSendaiSmall ruminant lentivirusesVaccineViral vectorSmall ruminant lentiviruses (SRLV) are responsible for significant economic losses in sheep and goat farming; however, effective vaccination strategies remain unavailable. This study evaluated the immunogenicity, safety, and protective efficacy of a recombinant Sendai virus vector (SeV) expressing SRLV gag-P25 (rSeV-GFP-P25) in lambs. Twenty-one SRLV-negative lambs were divided into three groups and inoculated intranasally thrice with culture medium (group 1); SeV-GFP (group 2) or rSeV-GFP-P25 (group 3). Lambs were challenged with homologous SRLV at 16 weeks post-first immunization. Clinical and hematological parameters, antibody responses, SRLV viral loads in peripheral blood mononuclear cells (PBMCs) and target tissues, histopathological and histomorphometric analyses, assisted with artificial intelligence, of interstitial pneumonia were assessed. No clinicopathological alterations were observed, except for a transient temperature increase in group 3 post-first immunization. Group 2 showed mild SeV-neutralizing antibodies, while rSeV-GFP-P25 (group 3) induced negligible SRLV-specific antibody responses. Group 3 exhibited higher SRLV DNA copies in PBMCs but lower in most SRLV target tissues compared to control groups, with no SRLV DNA detected in spleen and bone marrow. Histomorphometry revealed reduced alveolar septal thickening in group 3, indicating partial protection against early SRLV-associated interstitial pneumonia. These results warrant further investigation into cellular immunity and long-term protection.This study was funded by a grant from the Ministerio de Ciencia, Innovación y Universidades, Government of Spain under Grant RTI2018-096172-B-C33. Gobierno de Navarra (PC24-ECTIVAC-002-017-018). Álex Gómez is a PhD candidate funded by the Gobierno de Aragón [ORDEN CUS/803/2021]. We are grateful to Centro de Investigación Biomédica de Aragón (CIBA) for the assistance in the scanning of histopahological samples.Taylor & FrancisAgronomía, Biotecnología y AlimentaciónAgronomia, Bioteknologia eta ElikaduraGobierno de Navarra / Nafarroako Gobernua2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2454/56067reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096172-B-C33info:eu-repo/grantAgreement/Gobierno de Navarra// PC24-ECTIVAC-002-017-018© 2025 CSIC. Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/560672026-06-17T12:41:47Z
dc.title.none.fl_str_mv Immunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25
title Immunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25
spellingShingle Immunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25
Gómez, Álex
Intranasal
Sendai
Small ruminant lentiviruses
Vaccine
Viral vector
title_short Immunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25
title_full Immunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25
title_fullStr Immunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25
title_full_unstemmed Immunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25
title_sort Immunogenicity, security and protection against small ruminant lentivirus (SRLV) challenge in sheep, induced by intranasal immunization with a recombinant Sendai virus vector expressing SRLV gag-P25
dc.creator.none.fl_str_mv Gómez, Álex
Reina, R.
Glaría Ezquer, Idoia
Moncayola, Irati
Echeverría Garín, Irache
Rodríguez Largo, Ana
Blas, Ignacio de
Pérez, Estela
Pérez, Marta María
Villanueva-Saz, Sergio
Lee, Benhur
Diego, Alicia de
Miguel, Ricardo de
Luján, Lluís
author Gómez, Álex
author_facet Gómez, Álex
Reina, R.
Glaría Ezquer, Idoia
Moncayola, Irati
Echeverría Garín, Irache
Rodríguez Largo, Ana
Blas, Ignacio de
Pérez, Estela
Pérez, Marta María
Villanueva-Saz, Sergio
Lee, Benhur
Diego, Alicia de
Miguel, Ricardo de
Luján, Lluís
author_role author
author2 Reina, R.
Glaría Ezquer, Idoia
Moncayola, Irati
Echeverría Garín, Irache
Rodríguez Largo, Ana
Blas, Ignacio de
Pérez, Estela
Pérez, Marta María
Villanueva-Saz, Sergio
Lee, Benhur
Diego, Alicia de
Miguel, Ricardo de
Luján, Lluís
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agronomía, Biotecnología y Alimentación
Agronomia, Bioteknologia eta Elikadura
Gobierno de Navarra / Nafarroako Gobernua
dc.subject.none.fl_str_mv Intranasal
Sendai
Small ruminant lentiviruses
Vaccine
Viral vector
topic Intranasal
Sendai
Small ruminant lentiviruses
Vaccine
Viral vector
description Small ruminant lentiviruses (SRLV) are responsible for significant economic losses in sheep and goat farming; however, effective vaccination strategies remain unavailable. This study evaluated the immunogenicity, safety, and protective efficacy of a recombinant Sendai virus vector (SeV) expressing SRLV gag-P25 (rSeV-GFP-P25) in lambs. Twenty-one SRLV-negative lambs were divided into three groups and inoculated intranasally thrice with culture medium (group 1); SeV-GFP (group 2) or rSeV-GFP-P25 (group 3). Lambs were challenged with homologous SRLV at 16 weeks post-first immunization. Clinical and hematological parameters, antibody responses, SRLV viral loads in peripheral blood mononuclear cells (PBMCs) and target tissues, histopathological and histomorphometric analyses, assisted with artificial intelligence, of interstitial pneumonia were assessed. No clinicopathological alterations were observed, except for a transient temperature increase in group 3 post-first immunization. Group 2 showed mild SeV-neutralizing antibodies, while rSeV-GFP-P25 (group 3) induced negligible SRLV-specific antibody responses. Group 3 exhibited higher SRLV DNA copies in PBMCs but lower in most SRLV target tissues compared to control groups, with no SRLV DNA detected in spleen and bone marrow. Histomorphometry revealed reduced alveolar septal thickening in group 3, indicating partial protection against early SRLV-associated interstitial pneumonia. These results warrant further investigation into cellular immunity and long-term protection.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/56067
url https://hdl.handle.net/2454/56067
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096172-B-C33
info:eu-repo/grantAgreement/Gobierno de Navarra// PC24-ECTIVAC-002-017-018
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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repository.mail.fl_str_mv
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