Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPK

In response to foreign or endogenous stimuli, both microglia and astrocytes adopt an activated phenotype that promotes the release of pro-inflammatory mediators. This inflammatory mechanism, known as neuroinflammation, is essential in the defense against foreign invasion and in normal tissue repair;...

Descripción completa

Detalles Bibliográficos
Autores: Tomas-Hernandez, Sarah, Blanco, Jordi, Garcia Vallvé, Santiago, Pujadas, Gerard, Ojeda-Montes, María José, Gimeno, Aleix, Arola, Lluís, Minghetti, Luisa, Beltrán-Debón, Raúl, Mulero, Miquel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/53449
Acceso en línea:http://hdl.handle.net/10230/53449
http://dx.doi.org/10.3390/pharmaceutics13060806
Access Level:acceso abierto
Palabra clave:Gírgola de castanyer
Antiinflamatoris
Genètica
id ES_17f948dc6f9960fdc19a4cde455c9d8f
oai_identifier_str oai:recercat.cat:10230/53449
network_acronym_str ES
network_name_str España
repository_id_str
spelling Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPKTomas-Hernandez, SarahBlanco, JordiGarcia Vallvé, SantiagoPujadas, GerardOjeda-Montes, María JoséGimeno, AleixArola, LluísMinghetti, LuisaBeltrán-Debón, RaúlMulero, MiquelGírgola de castanyerAntiinflamatorisGenèticaIn response to foreign or endogenous stimuli, both microglia and astrocytes adopt an activated phenotype that promotes the release of pro-inflammatory mediators. This inflammatory mechanism, known as neuroinflammation, is essential in the defense against foreign invasion and in normal tissue repair; nevertheless, when constantly activated, this process can become detrimental through the release of neurotoxic factors that amplify underlying disease. In consequence, this study presents the anti-inflammatory and immunomodulatory properties of o-orsellinaldehyde, a natural compound found by an in silico approach in the Grifola frondosa mushroom, in astrocytes and microglia cells. For this purpose, primary microglia and astrocytes were isolated from mice brain and cultured in vitro. Subsequently, cells were exposed to LPS in the absence or presence of increasing concentrations of this natural compound. Specifically, the results shown that o-orsellinaldehyde strongly inhibits the LPS-induced inflammatory response in astrocytes and microglia by decreasing nitrite formation and downregulating iNOS and HO-1 expression. Furthermore, in microglia cells o-orsellinaldehyde inhibits NF-κB activation; and potently counteracts LPS-mediated p38 kinase and JNK phosphorylation (MAPK). In this regard, o-orsellinaldehyde treatment also induces a significant cell immunomodulation by repolarizing microglia toward the M2 anti-inflammatory phenotype. Altogether, these results could partially explain the reported beneficial effects of G. frondosa extracts on inflammatory conditions.Funding: A.G.’s contract was supported by grant 2015FI_B00655 from the Catalonia GovernmentMDPI202220222021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/53449http://dx.doi.org/10.3390/pharmaceutics13060806reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglés© 2021 by Sarah Tomas-Hernandez et al. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/534492026-05-29T05:05:01Z
dc.title.none.fl_str_mv Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPK
title Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPK
spellingShingle Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPK
Tomas-Hernandez, Sarah
Gírgola de castanyer
Antiinflamatoris
Genètica
title_short Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPK
title_full Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPK
title_fullStr Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPK
title_full_unstemmed Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPK
title_sort Anti-inflammatory and immunomodulatory effects of the grifola frondosa natural compound o-Orsellinaldehyde on LPS-Challenged murine primary glial cells. Roles of NF-κβ and MAPK
dc.creator.none.fl_str_mv Tomas-Hernandez, Sarah
Blanco, Jordi
Garcia Vallvé, Santiago
Pujadas, Gerard
Ojeda-Montes, María José
Gimeno, Aleix
Arola, Lluís
Minghetti, Luisa
Beltrán-Debón, Raúl
Mulero, Miquel
author Tomas-Hernandez, Sarah
author_facet Tomas-Hernandez, Sarah
Blanco, Jordi
Garcia Vallvé, Santiago
Pujadas, Gerard
Ojeda-Montes, María José
Gimeno, Aleix
Arola, Lluís
Minghetti, Luisa
Beltrán-Debón, Raúl
Mulero, Miquel
author_role author
author2 Blanco, Jordi
Garcia Vallvé, Santiago
Pujadas, Gerard
Ojeda-Montes, María José
Gimeno, Aleix
Arola, Lluís
Minghetti, Luisa
Beltrán-Debón, Raúl
Mulero, Miquel
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Gírgola de castanyer
Antiinflamatoris
Genètica
topic Gírgola de castanyer
Antiinflamatoris
Genètica
description In response to foreign or endogenous stimuli, both microglia and astrocytes adopt an activated phenotype that promotes the release of pro-inflammatory mediators. This inflammatory mechanism, known as neuroinflammation, is essential in the defense against foreign invasion and in normal tissue repair; nevertheless, when constantly activated, this process can become detrimental through the release of neurotoxic factors that amplify underlying disease. In consequence, this study presents the anti-inflammatory and immunomodulatory properties of o-orsellinaldehyde, a natural compound found by an in silico approach in the Grifola frondosa mushroom, in astrocytes and microglia cells. For this purpose, primary microglia and astrocytes were isolated from mice brain and cultured in vitro. Subsequently, cells were exposed to LPS in the absence or presence of increasing concentrations of this natural compound. Specifically, the results shown that o-orsellinaldehyde strongly inhibits the LPS-induced inflammatory response in astrocytes and microglia by decreasing nitrite formation and downregulating iNOS and HO-1 expression. Furthermore, in microglia cells o-orsellinaldehyde inhibits NF-κB activation; and potently counteracts LPS-mediated p38 kinase and JNK phosphorylation (MAPK). In this regard, o-orsellinaldehyde treatment also induces a significant cell immunomodulation by repolarizing microglia toward the M2 anti-inflammatory phenotype. Altogether, these results could partially explain the reported beneficial effects of G. frondosa extracts on inflammatory conditions.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/53449
http://dx.doi.org/10.3390/pharmaceutics13060806
url http://hdl.handle.net/10230/53449
http://dx.doi.org/10.3390/pharmaceutics13060806
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869403954662604800
score 15,812429

Similares en LA Referencia