Elovl2-Ablation leads to mitochondrial membrane fatty acid remodeling and reduced efficiency in mouse liver mitochondria

The fatty acid elongase elongation of very long-chain fatty acids protein 2 (ELOVL2) controls the elongation of polyunsaturated fatty acids (PUFA) producing precursors for omega-3, docosahexaenoic acid (DHA), and omega-6, docosapentaenoic acid (DPAn-6) in vivo. Expectedly, Elovl2-ablation drasticall...

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Autores: Gómez Rodríguez, Alexia, Talamonti, Emanuela, Naudí, Alba, Kalinovich, Anastasia V., Pauter, Anna M., Barja de Quiroga, Gustavo, Bengtsson, Tore, Jacobsson, Anders, Pamplona, Reinald, Shabalina, Irina G.
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/71806
Acceso en línea:https://hdl.handle.net/20.500.14352/71806
Access Level:acceso abierto
Palabra clave:591.1
599.32
Docosahexaenoic acid (DHA) deficiency
Mitochondrial function
Polyunsaturated fatty acids
Membrane permeabilization
Oxidative damage markers
Adenine nucleotide translocase
Fisiología animal (Biología)
Mamíferos
2401.13 Fisiología Animal
2401.18 Mamíferos
id ES_17f33ce6eb38dc0ebb4aecb758c8d4b5
oai_identifier_str oai:docta.ucm.es:20.500.14352/71806
network_acronym_str ES
network_name_str España
repository_id_str
spelling Elovl2-Ablation leads to mitochondrial membrane fatty acid remodeling and reduced efficiency in mouse liver mitochondriaGómez Rodríguez, AlexiaTalamonti, EmanuelaNaudí, AlbaKalinovich, Anastasia V.Pauter, Anna M.Barja de Quiroga, GustavoBengtsson, ToreJacobsson, AndersPamplona, ReinaldShabalina, Irina G.591.1599.32Docosahexaenoic acid (DHA) deficiencyMitochondrial functionPolyunsaturated fatty acidsMembrane permeabilizationOxidative damage markersAdenine nucleotide translocaseFisiología animal (Biología)Mamíferos2401.13 Fisiología Animal2401.18 MamíferosThe fatty acid elongase elongation of very long-chain fatty acids protein 2 (ELOVL2) controls the elongation of polyunsaturated fatty acids (PUFA) producing precursors for omega-3, docosahexaenoic acid (DHA), and omega-6, docosapentaenoic acid (DPAn-6) in vivo. Expectedly, Elovl2-ablation drastically reduced the DHA and DPAn-6 in liver mitochondrial membranes. Unexpectedly, however, total PUFAs levels decreased further than could be explained by Elovl2 ablation. The lipid peroxidation process was not involved in PUFAs reduction since malondialdehyde-lysine (MDAL) and other oxidative stress biomarkers were not enhanced. The content of mitochondrial respiratory chain proteins remained unchanged. Still, membrane remodeling was associated with the high voltage-dependent anion channel (VDAC) and adenine nucleotide translocase 2 (ANT2), a possible reflection of the increased demand on phospholipid transport to the mitochondria. Mitochondrial function was impaired despite preserved content of the respiratory chain proteins and the absence of oxidative damage. Oligomycin-insensitive oxygen consumption increased, and coefficients of respiratory control were reduced by 50%. The mitochondria became very sensitive to fatty acid-induced uncoupling and permeabilization, where ANT2 is involved. Mitochondrial volume and number of peroxisomes increased as revealed by transmission electron microscopy. In conclusion, the results imply that endogenous DHA production is vital for the normal function of mouse liver mitochondria and could be relevant not only for mice but also for human metabolismMDPIUniversidad Complutense de Madrid20222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/71806reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/718062026-06-02T12:44:21Z
dc.title.none.fl_str_mv Elovl2-Ablation leads to mitochondrial membrane fatty acid remodeling and reduced efficiency in mouse liver mitochondria
title Elovl2-Ablation leads to mitochondrial membrane fatty acid remodeling and reduced efficiency in mouse liver mitochondria
spellingShingle Elovl2-Ablation leads to mitochondrial membrane fatty acid remodeling and reduced efficiency in mouse liver mitochondria
Gómez Rodríguez, Alexia
591.1
599.32
Docosahexaenoic acid (DHA) deficiency
Mitochondrial function
Polyunsaturated fatty acids
Membrane permeabilization
Oxidative damage markers
Adenine nucleotide translocase
Fisiología animal (Biología)
Mamíferos
2401.13 Fisiología Animal
2401.18 Mamíferos
title_short Elovl2-Ablation leads to mitochondrial membrane fatty acid remodeling and reduced efficiency in mouse liver mitochondria
title_full Elovl2-Ablation leads to mitochondrial membrane fatty acid remodeling and reduced efficiency in mouse liver mitochondria
title_fullStr Elovl2-Ablation leads to mitochondrial membrane fatty acid remodeling and reduced efficiency in mouse liver mitochondria
title_full_unstemmed Elovl2-Ablation leads to mitochondrial membrane fatty acid remodeling and reduced efficiency in mouse liver mitochondria
title_sort Elovl2-Ablation leads to mitochondrial membrane fatty acid remodeling and reduced efficiency in mouse liver mitochondria
dc.creator.none.fl_str_mv Gómez Rodríguez, Alexia
Talamonti, Emanuela
Naudí, Alba
Kalinovich, Anastasia V.
Pauter, Anna M.
Barja de Quiroga, Gustavo
Bengtsson, Tore
Jacobsson, Anders
Pamplona, Reinald
Shabalina, Irina G.
author Gómez Rodríguez, Alexia
author_facet Gómez Rodríguez, Alexia
Talamonti, Emanuela
Naudí, Alba
Kalinovich, Anastasia V.
Pauter, Anna M.
Barja de Quiroga, Gustavo
Bengtsson, Tore
Jacobsson, Anders
Pamplona, Reinald
Shabalina, Irina G.
author_role author
author2 Talamonti, Emanuela
Naudí, Alba
Kalinovich, Anastasia V.
Pauter, Anna M.
Barja de Quiroga, Gustavo
Bengtsson, Tore
Jacobsson, Anders
Pamplona, Reinald
Shabalina, Irina G.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 591.1
599.32
Docosahexaenoic acid (DHA) deficiency
Mitochondrial function
Polyunsaturated fatty acids
Membrane permeabilization
Oxidative damage markers
Adenine nucleotide translocase
Fisiología animal (Biología)
Mamíferos
2401.13 Fisiología Animal
2401.18 Mamíferos
topic 591.1
599.32
Docosahexaenoic acid (DHA) deficiency
Mitochondrial function
Polyunsaturated fatty acids
Membrane permeabilization
Oxidative damage markers
Adenine nucleotide translocase
Fisiología animal (Biología)
Mamíferos
2401.13 Fisiología Animal
2401.18 Mamíferos
description The fatty acid elongase elongation of very long-chain fatty acids protein 2 (ELOVL2) controls the elongation of polyunsaturated fatty acids (PUFA) producing precursors for omega-3, docosahexaenoic acid (DHA), and omega-6, docosapentaenoic acid (DPAn-6) in vivo. Expectedly, Elovl2-ablation drastically reduced the DHA and DPAn-6 in liver mitochondrial membranes. Unexpectedly, however, total PUFAs levels decreased further than could be explained by Elovl2 ablation. The lipid peroxidation process was not involved in PUFAs reduction since malondialdehyde-lysine (MDAL) and other oxidative stress biomarkers were not enhanced. The content of mitochondrial respiratory chain proteins remained unchanged. Still, membrane remodeling was associated with the high voltage-dependent anion channel (VDAC) and adenine nucleotide translocase 2 (ANT2), a possible reflection of the increased demand on phospholipid transport to the mitochondria. Mitochondrial function was impaired despite preserved content of the respiratory chain proteins and the absence of oxidative damage. Oligomycin-insensitive oxygen consumption increased, and coefficients of respiratory control were reduced by 50%. The mitochondria became very sensitive to fatty acid-induced uncoupling and permeabilization, where ANT2 is involved. Mitochondrial volume and number of peroxisomes increased as revealed by transmission electron microscopy. In conclusion, the results imply that endogenous DHA production is vital for the normal function of mouse liver mitochondria and could be relevant not only for mice but also for human metabolism
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01
2022
2022-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/71806
url https://hdl.handle.net/20.500.14352/71806
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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