Short communication. An improved intersubspecific genetic map in Lens including functional markers
[EN] A previous Lensgenetic map was improved by adding 31 molecular genetic markers, reaching a total of 190 mar-kers with undistorted segregation. Data were obtained from the segregational analysis of 113 F2plants generated froma single hybrid of Lens culinarisssp. culinaris×L. c.ssp. orientalis. T...
| Autores: | , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2013 |
| País: | España |
| Institución: | Universidad de León |
| Repositorio: | BULERIA. Repositorio Institucional de la Universidad de León |
| OAI Identifier: | oai:buleria.unileon.es:10612/26125 |
| Acceso en línea: | https://sjar.revistas.csic.es/index.php/sjar/article/view/3283 https://hdl.handle.net/10612/26125 |
| Access Level: | acceso abierto |
| Palabra clave: | Botánica Genética Lens culinaris Lens orientalis Lentil Linkage map TFL1 2417 Biología Vegetal (Botánica) 2417.14 Genética Vegetal |
| Sumario: | [EN] A previous Lensgenetic map was improved by adding 31 molecular genetic markers, reaching a total of 190 mar-kers with undistorted segregation. Data were obtained from the segregational analysis of 113 F2plants generated froma single hybrid of Lens culinarisssp. culinaris×L. c.ssp. orientalis. The added markers are predominantly codomi-nant (15 SSRs, five CAPSs, four presence-absence polymorphisms, three length polymorphisms, two RAPDs, andtwo SRAPs). At a LOD score of 3.0, the 190 markers were grouped into eight linkage groups (LG) covering 2,234.4cM, with an average distance between markers of 12.28 cM. This linkage map has reduced the numbers of linkagegroups from ten in the previous map to eight. Most of the added markers must be functional markers since primerswere mostly designed to amplify transcribed sequences. Some of the amplicons were sequenced to test if they werefunctional markers. One of the sequences showed homology with the Pisum TFL1agene, involved in the transitionfrom vegetative to flowering stages. This lentil gene was located in the LG 1 thanks to the presence of a polymorphicmicrosatellite in the first intron of the gene. Since L. culinarisssp. orientalisis the primary source of additional ge-netic variability for lentil, this improved map could help in the use of such variability in lentil breeding programs |
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