Genotypic comparison of Pantoea agglomerans plant and clinical strains

Pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. However, commercial registration of P. agglomerans biocontrol products is hampered because this species is currently listed as...

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Autores: Rezzonico, Fabio, Smits, Theo H.M., Montesinos Seguí, Emilio, Frey, Jürg E., Duffy, Brion
Formato: artículo
Fecha de publicación:2009
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/3489
Acesso em linha:http://hdl.handle.net/10256/3489
Access Level:acceso abierto
Palavra-chave:Bacteris -- Control biològic
Plantes -- Malalties fúngiques
Virosis (Plantes)
Bacteria -- Biological control
Fungal diseases of plants
Virus diseases of plants
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spelling Genotypic comparison of Pantoea agglomerans plant and clinical strainsRezzonico, FabioSmits, Theo H.M.Montesinos Seguí, EmilioFrey, Jürg E.Duffy, BrionBacteris -- Control biològicPlantes -- Malalties fúngiquesVirosis (Plantes)Bacteria -- Biological controlFungal diseases of plantsVirus diseases of plantsPantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. However, commercial registration of P. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (BL2) organism due to clinical reports as an opportunistic human pathogen. This study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic markers using multi-locus phylogenetic analysis and fluorescent amplified fragment length polymorphisms (fAFLP) fingerprinting. Results: Majority of the clinical isolates from culture collections were found to be improperly designated as P. agglomerans after sequence analysis. The frequent taxonomic rearrangements underwent by the Enterobacter agglomerans/Erwinia herbicola complex may be a major problem in assessing clinical associations within P. agglomerans. In the P. agglomerans sensu stricto (in the stricter sense) group, there was no discrete clustering of clinical/biocontrol strains and no marker was identified that was uniquely associated to clinical strains. A putative biocontrol-specific fAFLP marker was identified only in biocontrol strains. The partial ORF located in this band corresponded to an ABC transporter that was found in all P. agglomerans strains. Conclusion: Taxonomic mischaracterization was identified as a major problem with P. agglomerans, and current techniques removed a majority of clinical strains from this species. Although clear discrimination between P. agglomerans plant and clinical strains was not obtained with phylogenetic analysis, a single marker characteristic of biocontrol strains was identified which may be of use in strain biosafety determinations. In addition, the lack of Koch's postulate fulfilment, rare retention of clinical strains for subsequent confirmation, and the polymicrobial nature of P. agglomerans clinical reports should be considered in biosafety assessment of beneficial strains in this speciesBioMed Central2009info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10256/3489http://hdl.handle.net/10256/3489BMC Microbiology, 2009, vol. 9, núm. 204Articles publicats (D-EQATA)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2180-9-204info:eu-repo/semantics/altIdentifier/issn/1471-2180Aquest document està subjecte a una llicència Creative Commons: Reconeixement (by)http://creativecommons.org/licenses/by/2.0/es/deed.cainfo:eu-repo/semantics/openAccessoai:recercat.cat:10256/34892026-05-29T05:05:01Z
dc.title.none.fl_str_mv Genotypic comparison of Pantoea agglomerans plant and clinical strains
title Genotypic comparison of Pantoea agglomerans plant and clinical strains
spellingShingle Genotypic comparison of Pantoea agglomerans plant and clinical strains
Rezzonico, Fabio
Bacteris -- Control biològic
Plantes -- Malalties fúngiques
Virosis (Plantes)
Bacteria -- Biological control
Fungal diseases of plants
Virus diseases of plants
title_short Genotypic comparison of Pantoea agglomerans plant and clinical strains
title_full Genotypic comparison of Pantoea agglomerans plant and clinical strains
title_fullStr Genotypic comparison of Pantoea agglomerans plant and clinical strains
title_full_unstemmed Genotypic comparison of Pantoea agglomerans plant and clinical strains
title_sort Genotypic comparison of Pantoea agglomerans plant and clinical strains
dc.creator.none.fl_str_mv Rezzonico, Fabio
Smits, Theo H.M.
Montesinos Seguí, Emilio
Frey, Jürg E.
Duffy, Brion
author Rezzonico, Fabio
author_facet Rezzonico, Fabio
Smits, Theo H.M.
Montesinos Seguí, Emilio
Frey, Jürg E.
Duffy, Brion
author_role author
author2 Smits, Theo H.M.
Montesinos Seguí, Emilio
Frey, Jürg E.
Duffy, Brion
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bacteris -- Control biològic
Plantes -- Malalties fúngiques
Virosis (Plantes)
Bacteria -- Biological control
Fungal diseases of plants
Virus diseases of plants
topic Bacteris -- Control biològic
Plantes -- Malalties fúngiques
Virosis (Plantes)
Bacteria -- Biological control
Fungal diseases of plants
Virus diseases of plants
description Pantoea agglomerans strains are among the most promising biocontrol agents for a variety of bacterial and fungal plant diseases, particularly fire blight of apple and pear. However, commercial registration of P. agglomerans biocontrol products is hampered because this species is currently listed as a biosafety level 2 (BL2) organism due to clinical reports as an opportunistic human pathogen. This study compares plant-origin and clinical strains in a search for discriminating genotypic/phenotypic markers using multi-locus phylogenetic analysis and fluorescent amplified fragment length polymorphisms (fAFLP) fingerprinting. Results: Majority of the clinical isolates from culture collections were found to be improperly designated as P. agglomerans after sequence analysis. The frequent taxonomic rearrangements underwent by the Enterobacter agglomerans/Erwinia herbicola complex may be a major problem in assessing clinical associations within P. agglomerans. In the P. agglomerans sensu stricto (in the stricter sense) group, there was no discrete clustering of clinical/biocontrol strains and no marker was identified that was uniquely associated to clinical strains. A putative biocontrol-specific fAFLP marker was identified only in biocontrol strains. The partial ORF located in this band corresponded to an ABC transporter that was found in all P. agglomerans strains. Conclusion: Taxonomic mischaracterization was identified as a major problem with P. agglomerans, and current techniques removed a majority of clinical strains from this species. Although clear discrimination between P. agglomerans plant and clinical strains was not obtained with phylogenetic analysis, a single marker characteristic of biocontrol strains was identified which may be of use in strain biosafety determinations. In addition, the lack of Koch's postulate fulfilment, rare retention of clinical strains for subsequent confirmation, and the polymicrobial nature of P. agglomerans clinical reports should be considered in biosafety assessment of beneficial strains in this species
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/3489
http://hdl.handle.net/10256/3489
url http://hdl.handle.net/10256/3489
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/1471-2180-9-204
info:eu-repo/semantics/altIdentifier/issn/1471-2180
dc.rights.none.fl_str_mv Aquest document està subjecte a una llicència Creative Commons: Reconeixement (by)
http://creativecommons.org/licenses/by/2.0/es/deed.ca
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Aquest document està subjecte a una llicència Creative Commons: Reconeixement (by)
http://creativecommons.org/licenses/by/2.0/es/deed.ca
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv BMC Microbiology, 2009, vol. 9, núm. 204
Articles publicats (D-EQATA)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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