Phage lytic proteins: a natural approach to agro-food safety

Sustainability is a leading trend in the context of food production. Additionally, consumers increasingly demand safer and less-processed products. Among the different technologies used to maintain the quality and extend the shelf-life of fresh and minimally-processed food, natural antimicrobial age...

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Detalles Bibliográficos
Autores: Agún, Seila, Fernández Llamas, Lucía, Rodríguez González, Ana, García Suárez, María Pilar
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/381904
Acceso en línea:http://hdl.handle.net/10261/381904
Access Level:acceso abierto
Palabra clave:Food safety
Endolysins
Bacteriophages
Antimicrobials
Foodborne pathogens
Regulatory burdens
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spelling Phage lytic proteins: a natural approach to agro-food safetyAgún, SeilaFernández Llamas, LucíaRodríguez González, AnaGarcía Suárez, María PilarFood safetyEndolysinsBacteriophagesAntimicrobialsFoodborne pathogensRegulatory burdensSustainability is a leading trend in the context of food production. Additionally, consumers increasingly demand safer and less-processed products. Among the different technologies used to maintain the quality and extend the shelf-life of fresh and minimally-processed food, natural antimicrobial agents offer a promising strategy to replace conventional compounds. In this regard, phage lytic proteins or lysins, such as endolysins and virion-associated peptidoglycan hydrolases (VAPGHs), have been proposed as a viable option for the avoidance and elimination of undesirable bacteria within the food production chain. Even when applied exogenously, these proteins can degrade the bacterial cell wall maintaining their lytic activity. This feature, alongside their modular structure, which can be exploited for bioengineering, provides significant biotechnological potential. However, despite the promising properties of lysins, the main obstacle for their commercialization is the limited legal information regulating their use. This challenge underscores the need to navigate complex regulatory pathways. The primary objective of this review is to address this crucial gap and summarize the many prospective applications of endolysins during the different stages of food production. By doing so, we aim to provide clarity and insight into the regulatory challenges that must be overcome for the successful utilization of lysins.This work was funded by grants PID2019-105311RB-I00 (MICIU/AEI/FEDER, UE, Spain) to P.G. and A.R., and AYUD/2021/52120 (Program of Science, Technology and Innovation 2021-2023 and FEDER EU, Principado de Asturias, Spain). S.A. has an FPI fellowship (Ministry of Science and Innovation, Spain).Peer reviewedSciopenTsinghua University PressAgencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Principado de AsturiasGarcía Suárez, María Pilar [0000-0003-1213-8165]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_dcae04bcPublisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/381904reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105311RB-I00https://doi.org/10.26599/FSHW.2024.9250128Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3819042026-05-22T06:33:51Z
dc.title.none.fl_str_mv Phage lytic proteins: a natural approach to agro-food safety
title Phage lytic proteins: a natural approach to agro-food safety
spellingShingle Phage lytic proteins: a natural approach to agro-food safety
Agún, Seila
Food safety
Endolysins
Bacteriophages
Antimicrobials
Foodborne pathogens
Regulatory burdens
title_short Phage lytic proteins: a natural approach to agro-food safety
title_full Phage lytic proteins: a natural approach to agro-food safety
title_fullStr Phage lytic proteins: a natural approach to agro-food safety
title_full_unstemmed Phage lytic proteins: a natural approach to agro-food safety
title_sort Phage lytic proteins: a natural approach to agro-food safety
dc.creator.none.fl_str_mv Agún, Seila
Fernández Llamas, Lucía
Rodríguez González, Ana
García Suárez, María Pilar
author Agún, Seila
author_facet Agún, Seila
Fernández Llamas, Lucía
Rodríguez González, Ana
García Suárez, María Pilar
author_role author
author2 Fernández Llamas, Lucía
Rodríguez González, Ana
García Suárez, María Pilar
author2_role author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Principado de Asturias
García Suárez, María Pilar [0000-0003-1213-8165]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Food safety
Endolysins
Bacteriophages
Antimicrobials
Foodborne pathogens
Regulatory burdens
topic Food safety
Endolysins
Bacteriophages
Antimicrobials
Foodborne pathogens
Regulatory burdens
description Sustainability is a leading trend in the context of food production. Additionally, consumers increasingly demand safer and less-processed products. Among the different technologies used to maintain the quality and extend the shelf-life of fresh and minimally-processed food, natural antimicrobial agents offer a promising strategy to replace conventional compounds. In this regard, phage lytic proteins or lysins, such as endolysins and virion-associated peptidoglycan hydrolases (VAPGHs), have been proposed as a viable option for the avoidance and elimination of undesirable bacteria within the food production chain. Even when applied exogenously, these proteins can degrade the bacterial cell wall maintaining their lytic activity. This feature, alongside their modular structure, which can be exploited for bioengineering, provides significant biotechnological potential. However, despite the promising properties of lysins, the main obstacle for their commercialization is the limited legal information regulating their use. This challenge underscores the need to navigate complex regulatory pathways. The primary objective of this review is to address this crucial gap and summarize the many prospective applications of endolysins during the different stages of food production. By doing so, we aim to provide clarity and insight into the regulatory challenges that must be overcome for the successful utilization of lysins.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_dcae04bc
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/381904
url http://hdl.handle.net/10261/381904
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105311RB-I00
https://doi.org/10.26599/FSHW.2024.9250128
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sciopen
Tsinghua University Press
publisher.none.fl_str_mv Sciopen
Tsinghua University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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