The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery

ATP-Binding Cassette E (ABCE) proteins dissociate cytoplasmic ribosomes after translation terminates, and contribute to ribosome recycling, thus linking translation termination to initiation. This function has been demonstrated to be essential in animals, fungi, and archaea, but remains unexplored i...

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Authors: Navarro-Quiles, Carla, Mateo-Bonmatı, Eduardo, Candela, Héctor, Robles, Pedro, Martínez-Laborda, Antonio, Fernández, Yolanda, Simura, Jan, Ljung, Karin, Rubio, Vicente, Ponce, María Rosa, Micol, José Luis
Format: article
Publication Date:2022
Country:España
Institution:Universidad Miguel Hernández de Elche
Repository:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/38363
Online Access:https://hdl.handle.net/11000/38363
Access Level:Open access
Keyword:Arabidopsis ABCE2
ribosome recycling
translation machinery
venation pattern
auxin metabolism
CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
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spelling The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machineryNavarro-Quiles, CarlaMateo-Bonmatı, EduardoCandela, HéctorRobles, PedroMartínez-Laborda, AntonioFernández, YolandaSimura, JanLjung, KarinRubio, VicentePonce, María RosaMicol, José LuisArabidopsis ABCE2ribosome recyclingtranslation machineryvenation patternauxin metabolismCDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. CitologíaATP-Binding Cassette E (ABCE) proteins dissociate cytoplasmic ribosomes after translation terminates, and contribute to ribosome recycling, thus linking translation termination to initiation. This function has been demonstrated to be essential in animals, fungi, and archaea, but remains unexplored in plants. In most species, ABCE is encoded by a single-copy gene; by contrast, Arabidopsis thaliana has two ABCE paralogs, of which ABCE2 seems to conserve the ancestral function. We isolated apiculata7-1 (api7-1), the first viable, hypomorphic allele of ABCE2, which has a pleiotropic morphological phenotype reminiscent of mutations affecting ribosome biogenesis factors and ribosomal proteins. We also studied api7-2, a null, recessive lethal allele of ABCE2. Co-immunoprecipitation experiments showed that ABCE2 physically interacts with components of the translation machinery. An RNA-seq study of the api7-1 mutant showed increased responses to iron and sulfur starvation. We also found increased transcript levels of genes related to auxin signaling and metabolism. Our results support for the first time a conserved role for ABCE proteins in translation in plants, as previously shown for the animal, fungal, and archaeal lineages. In Arabidopsis, the ABCE2 protein seems important for general growth and vascular development, likely due to an indirect effect through auxin metabolismFrontiers MediaDepartamentos de la UMH::Biología Aplicada202520252022info:eu-repo/semantics/articleapplication/pdf17application/pdfhttps://hdl.handle.net/11000/38363reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheIngléshttps://doi.org/10.3389/fpls.2022.1009895info:eu-repo/semantics/openAccessAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/oai:dspace.umh.es:11000/383632026-05-27T13:36:21Z
dc.title.none.fl_str_mv The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery
title The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery
spellingShingle The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery
Navarro-Quiles, Carla
Arabidopsis ABCE2
ribosome recycling
translation machinery
venation pattern
auxin metabolism
CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
title_short The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery
title_full The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery
title_fullStr The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery
title_full_unstemmed The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery
title_sort The Arabidopsis ATP-Binding Cassette E protein ABCE2 is a conserved component of the translation machinery
dc.creator.none.fl_str_mv Navarro-Quiles, Carla
Mateo-Bonmatı, Eduardo
Candela, Héctor
Robles, Pedro
Martínez-Laborda, Antonio
Fernández, Yolanda
Simura, Jan
Ljung, Karin
Rubio, Vicente
Ponce, María Rosa
Micol, José Luis
author Navarro-Quiles, Carla
author_facet Navarro-Quiles, Carla
Mateo-Bonmatı, Eduardo
Candela, Héctor
Robles, Pedro
Martínez-Laborda, Antonio
Fernández, Yolanda
Simura, Jan
Ljung, Karin
Rubio, Vicente
Ponce, María Rosa
Micol, José Luis
author_role author
author2 Mateo-Bonmatı, Eduardo
Candela, Héctor
Robles, Pedro
Martínez-Laborda, Antonio
Fernández, Yolanda
Simura, Jan
Ljung, Karin
Rubio, Vicente
Ponce, María Rosa
Micol, José Luis
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Biología Aplicada
dc.subject.none.fl_str_mv Arabidopsis ABCE2
ribosome recycling
translation machinery
venation pattern
auxin metabolism
CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
topic Arabidopsis ABCE2
ribosome recycling
translation machinery
venation pattern
auxin metabolism
CDU::5 - Ciencias puras y naturales::57 - Biología::576 - Biología celular y subcelular. Citología
description ATP-Binding Cassette E (ABCE) proteins dissociate cytoplasmic ribosomes after translation terminates, and contribute to ribosome recycling, thus linking translation termination to initiation. This function has been demonstrated to be essential in animals, fungi, and archaea, but remains unexplored in plants. In most species, ABCE is encoded by a single-copy gene; by contrast, Arabidopsis thaliana has two ABCE paralogs, of which ABCE2 seems to conserve the ancestral function. We isolated apiculata7-1 (api7-1), the first viable, hypomorphic allele of ABCE2, which has a pleiotropic morphological phenotype reminiscent of mutations affecting ribosome biogenesis factors and ribosomal proteins. We also studied api7-2, a null, recessive lethal allele of ABCE2. Co-immunoprecipitation experiments showed that ABCE2 physically interacts with components of the translation machinery. An RNA-seq study of the api7-1 mutant showed increased responses to iron and sulfur starvation. We also found increased transcript levels of genes related to auxin signaling and metabolism. Our results support for the first time a conserved role for ABCE proteins in translation in plants, as previously shown for the animal, fungal, and archaeal lineages. In Arabidopsis, the ABCE2 protein seems important for general growth and vascular development, likely due to an indirect effect through auxin metabolism
publishDate 2022
dc.date.none.fl_str_mv 2022
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/11000/38363
url https://hdl.handle.net/11000/38363
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3389/fpls.2022.1009895
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.format.none.fl_str_mv application/pdf
17
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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