yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets

© The Author(s).

Detalles Bibliográficos
Autores: Vázquez-Marín, Javier, Gutiérrez-Triana, José Arturo, Almuedo-Castillo, María, Buono, Lorena, Gómez-Skarmeta, José Luis, Mateo, Juan Luis, Wittbrodt, Joachim, Martínez-Morales, Juan Ramón
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/202571
Acceso en línea:http://hdl.handle.net/10261/202571
Access Level:acceso abierto
Palabra clave:Yap/Taz
Chromatin binding
Morphogenesis
Apoptosis
Transcriptional activation domain
DamID-seq
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repository_id_str
spelling yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targetsVázquez-Marín, JavierGutiérrez-Triana, José ArturoAlmuedo-Castillo, MaríaBuono, LorenaGómez-Skarmeta, José LuisMateo, Juan LuisWittbrodt, JoachimMartínez-Morales, Juan RamónYap/TazChromatin bindingMorphogenesisApoptosisTranscriptional activation domainDamID-seq© The Author(s).Yap1/Taz are well-known Hippo effectors triggering complex transcriptional programs controlling growth, survival and cancer progression. Here, we describe yap1b, a new Yap1/Taz family member with a unique transcriptional activation domain that cannot be phosphorylated by Src/Yes kinases. We show that yap1b evolved specifically in euteleosts (i.e. including medaka but not zebrafish) by duplication and adaptation of yap1. Using DamID-seq, we generated maps of chromatin occupancy for Yap1, Taz (Wwtr1) and Yap1b in gastrulating zebrafish and medaka embryos. Our comparative analyses uncover the genetic programs controlled by Yap family proteins during early embryogenesis, and show largely overlapping targets for Yap1 and Yap1b. CRISPR/Cas9-induced mutation of yap1b in medaka does not result in an overt phenotype during embryogenesis or adulthood. However, yap1b mutation strongly enhances the embryonic malformations observed in yap1 mutants. Thus yap1−/−; yap1b−/− double mutants display more severe body flattening, eye misshaping and increased apoptosis than yap1−/− single mutants, thus revealing overlapping gene functions. Our results indicate that, despite its divergent transactivation domain, Yap1b cooperates with Yap1 to regulate cell survival and tissue morphogenesis during early development.This work was supported by grants from the Ministerio de Economía, Industria y Competitividad de Espanña (BFU2014-53765-P, BFU2016- 81887-REDT, BFU2017-86339-P and MDM-2016-0687 to J.R.M.-M.).Company of BiologistsMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/202571reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-53765-PBFU2017-86339-P/AEI/10.13039/501100011033info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-81887-REDTinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BFU2017-86339-Pinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MDM-2016-0687BFU2017-86339-P/AEI/10.13039/501100011033http://dx.doi.org/10.1242/dev.173286Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2025712026-05-22T06:33:51Z
dc.title.none.fl_str_mv yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets
title yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets
spellingShingle yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets
Vázquez-Marín, Javier
Yap/Taz
Chromatin binding
Morphogenesis
Apoptosis
Transcriptional activation domain
DamID-seq
title_short yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets
title_full yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets
title_fullStr yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets
title_full_unstemmed yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets
title_sort yap1b, a divergent Yap/Taz family member, cooperates with yap1 in survival and morphogenesis via common transcriptional targets
dc.creator.none.fl_str_mv Vázquez-Marín, Javier
Gutiérrez-Triana, José Arturo
Almuedo-Castillo, María
Buono, Lorena
Gómez-Skarmeta, José Luis
Mateo, Juan Luis
Wittbrodt, Joachim
Martínez-Morales, Juan Ramón
author Vázquez-Marín, Javier
author_facet Vázquez-Marín, Javier
Gutiérrez-Triana, José Arturo
Almuedo-Castillo, María
Buono, Lorena
Gómez-Skarmeta, José Luis
Mateo, Juan Luis
Wittbrodt, Joachim
Martínez-Morales, Juan Ramón
author_role author
author2 Gutiérrez-Triana, José Arturo
Almuedo-Castillo, María
Buono, Lorena
Gómez-Skarmeta, José Luis
Mateo, Juan Luis
Wittbrodt, Joachim
Martínez-Morales, Juan Ramón
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Yap/Taz
Chromatin binding
Morphogenesis
Apoptosis
Transcriptional activation domain
DamID-seq
topic Yap/Taz
Chromatin binding
Morphogenesis
Apoptosis
Transcriptional activation domain
DamID-seq
description © The Author(s).
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/202571
url http://hdl.handle.net/10261/202571
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-53765-P
BFU2017-86339-P/AEI/10.13039/501100011033
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2016-81887-REDT
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BFU2017-86339-P
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/MDM-2016-0687
BFU2017-86339-P/AEI/10.13039/501100011033
http://dx.doi.org/10.1242/dev.173286
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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