Isolation Methods of Peripheral Blood Mononuclear Cells in Spanish Biobanks: An Overview

The Spanish Hematic Derivatives Group, consisting of 26 biobanks, was established in 2011. We describe here the viability results of our publically available standard operating procedure to freeze and thaw peripheral blood mononuclear cells (PBMCs). Our protocol maximizes PBMC viability while avoidi...

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Detalles Bibliográficos
Autores: Consuegra Fernández, Irene|||/items/d0c1cf15-72e2-457b-93e8-518f29dc336a, Rodríguez-Aierbe, Clara, Santiuste, Inés, Bosch, Anna, Martínez-Marín, Rosario, Fortuto, María Antonia, Díaz, Tatiana, Martí , Salvador, Muñoz-Fernández, María Ángeles
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universidad Alfonso X el Sabio
Repositorio:Repositorio Institucional de la Universidad Alfonso X el Sabio
Idioma:inglés
OAI Identifier:oai:archive.uax.com:20.500.12080/54005
Acceso en línea:https://hdl.handle.net/20.500.12080/54005
Access Level:acceso abierto
Palabra clave:SOP
Biobancos
Criopreservación
Descripción
Sumario:The Spanish Hematic Derivatives Group, consisting of 26 biobanks, was established in 2011. We describe here the viability results of our publically available standard operating procedure to freeze and thaw peripheral blood mononuclear cells (PBMCs). Our protocol maximizes PBMC viability while avoiding where possible inter biobank and intrabiobank assay variability.