Activation of r20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium

In the human pathogen Mycoplasma genitalium, homologous recombination is under the control of r, an alternative sigma factor that boosts the generation of genetic and antigenic diversity in the population. Under laboratory growth conditions, r activation is rare and the factors governing its intermi...

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Autores: Torres Puig, Sergi|||0000-0002-8976-6488, Martínez Torró, Carlos, Granero Moya, Ignasi, Querol Murillo, Enrique|||0000-0002-3658-3434, Piñol Ribas, Jaume|||0000-0002-9055-8934, Pich, Òscar M.
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:225204
Acceso en línea:https://ddd.uab.cat/record/225204
https://dx.doi.org/urn:doi:10.1093/dnares/dsy011
Access Level:acceso abierto
Palabra clave:Mycoplasma
Sigma factor
Homologous recombination
Antigenic variation
Horizontal gene transferS
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spelling Activation of r20-dependent recombination and horizontal gene transfer in Mycoplasma genitaliumTorres Puig, Sergi|||0000-0002-8976-6488Martínez Torró, CarlosGranero Moya, IgnasiQuerol Murillo, Enrique|||0000-0002-3658-3434Piñol Ribas, Jaume|||0000-0002-9055-8934Pich, Òscar M.MycoplasmaSigma factorHomologous recombinationAntigenic variationHorizontal gene transferSIn the human pathogen Mycoplasma genitalium, homologous recombination is under the control of r, an alternative sigma factor that boosts the generation of genetic and antigenic diversity in the population. Under laboratory growth conditions, r activation is rare and the factors governing its intermittent activity are unknown. Two r-regulated genes, rrlA and rrlB, showed to be important for recombination of homologous DNA sequences in this bacterium. Herein, we demonstrate that rrlA and rrlB code for two small proteins that participate in a feed-forward loop essential for r function. In addition, we identify novel genes regulated by r and show that several non-coding regions, which function as a reservoir for the generation of antigenic diversity, are also activated by this alternative sigma factor. Finally, we reveal that M. genitalium cells can transfer DNA horizontally by a novel mechanism that requires RecA and is facilitated by r overexpression. This DNA transfer system is arguably fundamental for persistence of M. genitalium within the host since it could facilitate a rapid dissemination of successful antigenic variants within the population. Overall, these findings impose a novel conception of genome evolution, genetic variation and survival of M. genitalium within the host 22018-01-0120182018-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/225204https://dx.doi.org/urn:doi:10.1093/dnares/dsy011reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 BIO2013-48704-RMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 BFU2013-50176-EXPopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2252042026-06-06T12:50:31Z
dc.title.none.fl_str_mv Activation of r20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium
title Activation of r20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium
spellingShingle Activation of r20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium
Torres Puig, Sergi|||0000-0002-8976-6488
Mycoplasma
Sigma factor
Homologous recombination
Antigenic variation
Horizontal gene transferS
title_short Activation of r20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium
title_full Activation of r20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium
title_fullStr Activation of r20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium
title_full_unstemmed Activation of r20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium
title_sort Activation of r20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium
dc.creator.none.fl_str_mv Torres Puig, Sergi|||0000-0002-8976-6488
Martínez Torró, Carlos
Granero Moya, Ignasi
Querol Murillo, Enrique|||0000-0002-3658-3434
Piñol Ribas, Jaume|||0000-0002-9055-8934
Pich, Òscar M.
author Torres Puig, Sergi|||0000-0002-8976-6488
author_facet Torres Puig, Sergi|||0000-0002-8976-6488
Martínez Torró, Carlos
Granero Moya, Ignasi
Querol Murillo, Enrique|||0000-0002-3658-3434
Piñol Ribas, Jaume|||0000-0002-9055-8934
Pich, Òscar M.
author_role author
author2 Martínez Torró, Carlos
Granero Moya, Ignasi
Querol Murillo, Enrique|||0000-0002-3658-3434
Piñol Ribas, Jaume|||0000-0002-9055-8934
Pich, Òscar M.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Mycoplasma
Sigma factor
Homologous recombination
Antigenic variation
Horizontal gene transferS
topic Mycoplasma
Sigma factor
Homologous recombination
Antigenic variation
Horizontal gene transferS
description In the human pathogen Mycoplasma genitalium, homologous recombination is under the control of r, an alternative sigma factor that boosts the generation of genetic and antigenic diversity in the population. Under laboratory growth conditions, r activation is rare and the factors governing its intermittent activity are unknown. Two r-regulated genes, rrlA and rrlB, showed to be important for recombination of homologous DNA sequences in this bacterium. Herein, we demonstrate that rrlA and rrlB code for two small proteins that participate in a feed-forward loop essential for r function. In addition, we identify novel genes regulated by r and show that several non-coding regions, which function as a reservoir for the generation of antigenic diversity, are also activated by this alternative sigma factor. Finally, we reveal that M. genitalium cells can transfer DNA horizontally by a novel mechanism that requires RecA and is facilitated by r overexpression. This DNA transfer system is arguably fundamental for persistence of M. genitalium within the host since it could facilitate a rapid dissemination of successful antigenic variants within the population. Overall, these findings impose a novel conception of genome evolution, genetic variation and survival of M. genitalium within the host
publishDate 2018
dc.date.none.fl_str_mv 2
2018-01-01
2018
2018-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/225204
https://dx.doi.org/urn:doi:10.1093/dnares/dsy011
url https://ddd.uab.cat/record/225204
https://dx.doi.org/urn:doi:10.1093/dnares/dsy011
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BIO2013-48704-R
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 BFU2013-50176-EXP
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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