Genetic analysis of laminin A reveals diverse functions during morphogenesis in Drosophila

In order to dissect the functions of laminin A in vivo, we have undertaken a molecular and genetic characterization of the laminin A subunit (lamA) gene in Drosophila. Sequence analysis predicts a multidomain structure similar to mammalian homologs. We generated a series of complete and partial loss...

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Detalles Bibliográficos
Autores: Henchcliffe, Claire, García-Alonso, Luis, Tang, Joyce, Goodman, Corey S.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1993
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/308251
Acceso en línea:http://hdl.handle.net/10261/308251
Access Level:acceso abierto
Palabra clave:laminin A
Drosophila genetics
Cell fate
Limb structure
Wing structure
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spelling Genetic analysis of laminin A reveals diverse functions during morphogenesis in DrosophilaHenchcliffe, ClaireGarcía-Alonso, LuisTang, JoyceGoodman, Corey S.laminin ADrosophila geneticsCell fateLimb structureWing structureIn order to dissect the functions of laminin A in vivo, we have undertaken a molecular and genetic characterization of the laminin A subunit (lamA) gene in Drosophila. Sequence analysis predicts a multidomain structure similar to mammalian homologs. We generated a series of complete and partial loss-of-function mutant alleles of the lamA gene; complete loss-of-function mutations lead to late embryonic lethality. Certain combinations of partial loss-of-function lamA alleles give rise to escaper adults, which have rough eyes associated with changes in cell fate and pattern, misshapen legs and defects in wing structure. These phenotypes suggest that laminin A has diverse functions during morphogenesis in Drosophila.C. H. was a Lucille P. Markey Visiting Fellow and this work was supported in part by a grant from the Lucille P. Markey Charitable Trust. L. G-A. was an EMBO Postdoctoral Fellow and a HHMI Fellow. C. S. G. is an Investigator with the Howard Hughes Medical Institute.Peer reviewedCompany of BiologistsEMBOHoward Hughes Medical Institute202320231993info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/308251reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésDevelopmenthttps://doi.org/10.1242/dev.118.2.325Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3082512026-05-22T06:33:51Z
dc.title.none.fl_str_mv Genetic analysis of laminin A reveals diverse functions during morphogenesis in Drosophila
title Genetic analysis of laminin A reveals diverse functions during morphogenesis in Drosophila
spellingShingle Genetic analysis of laminin A reveals diverse functions during morphogenesis in Drosophila
Henchcliffe, Claire
laminin A
Drosophila genetics
Cell fate
Limb structure
Wing structure
title_short Genetic analysis of laminin A reveals diverse functions during morphogenesis in Drosophila
title_full Genetic analysis of laminin A reveals diverse functions during morphogenesis in Drosophila
title_fullStr Genetic analysis of laminin A reveals diverse functions during morphogenesis in Drosophila
title_full_unstemmed Genetic analysis of laminin A reveals diverse functions during morphogenesis in Drosophila
title_sort Genetic analysis of laminin A reveals diverse functions during morphogenesis in Drosophila
dc.creator.none.fl_str_mv Henchcliffe, Claire
García-Alonso, Luis
Tang, Joyce
Goodman, Corey S.
author Henchcliffe, Claire
author_facet Henchcliffe, Claire
García-Alonso, Luis
Tang, Joyce
Goodman, Corey S.
author_role author
author2 García-Alonso, Luis
Tang, Joyce
Goodman, Corey S.
author2_role author
author
author
dc.contributor.none.fl_str_mv EMBO
Howard Hughes Medical Institute
dc.subject.none.fl_str_mv laminin A
Drosophila genetics
Cell fate
Limb structure
Wing structure
topic laminin A
Drosophila genetics
Cell fate
Limb structure
Wing structure
description In order to dissect the functions of laminin A in vivo, we have undertaken a molecular and genetic characterization of the laminin A subunit (lamA) gene in Drosophila. Sequence analysis predicts a multidomain structure similar to mammalian homologs. We generated a series of complete and partial loss-of-function mutant alleles of the lamA gene; complete loss-of-function mutations lead to late embryonic lethality. Certain combinations of partial loss-of-function lamA alleles give rise to escaper adults, which have rough eyes associated with changes in cell fate and pattern, misshapen legs and defects in wing structure. These phenotypes suggest that laminin A has diverse functions during morphogenesis in Drosophila.
publishDate 1993
dc.date.none.fl_str_mv 1993
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/308251
url http://hdl.handle.net/10261/308251
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Development
https://doi.org/10.1242/dev.118.2.325
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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