Molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease

The spleen is a highly perfused organ involved in the immunological control and elimination of vector-borne pathogens (VBP), which could have a fundamental role in the pathogenesis of splenic disease. This study aimed to evaluate certain VBP in samples from dogs with splenic lesions. Seventy-seven E...

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Autores: Movilla, Rebeca, Altet Sanahujes, Laura, Serrano, Lorena, Tabar, Maria Dolores, Roura, Xavier|||0000-0002-1098-2381
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:186179
Acceso en línea:https://ddd.uab.cat/record/186179
https://dx.doi.org/urn:doi:10.1186/s13071-017-2074-z
Access Level:acceso abierto
Palabra clave:Canine
Vector-borne disease
Spleen
Polymerase chain reaction
Hemangiosarcoma
Babesia canis
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spelling Molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic diseaseMovilla, RebecaAltet Sanahujes, LauraSerrano, LorenaTabar, Maria DoloresRoura, Xavier|||0000-0002-1098-2381CanineVector-borne diseaseSpleenPolymerase chain reactionHemangiosarcomaBabesia canisThe spleen is a highly perfused organ involved in the immunological control and elimination of vector-borne pathogens (VBP), which could have a fundamental role in the pathogenesis of splenic disease. This study aimed to evaluate certain VBP in samples from dogs with splenic lesions. Seventy-seven EDTA-blood and 64 splenic tissue samples were collected from 78 dogs with splenic disease in a Mediterranean area. Babesia spp., Bartonella spp., Ehrlichia/Anaplasma spp., Hepatozoon canis, Leishmania infantum, hemotropic Mycoplasma spp. and Rickettsia spp. were targeted using PCR assays. Sixty EDTA-blood samples from dogs without evidence of splenic lesions were included as a control group. More than half (51.56%) of the biopsies (33/64) were consistent with benign lesions and 48.43% (31/64) with malignancy, mostly hemangiosarcoma (25/31). PCR yielded positive results in 13 dogs with spleen alterations (16.67%), for Babesia canis (n = 3), Babesia gibsoni (n = 2), hemotropic Mycoplasma spp. (n = 2), Rickettsia massiliae (n = 1) and "Babesia vulpes" (n = 1), in blood; and for B. canis, B. gibsoni, Ehrlichia canis and L. infantum (n = 1 each), in spleen. Two control dogs (3.3%) were positive for B. gibsoni and H. canis (n = 1 each). Benign lesions were detected in the 61.54% of infected dogs (8/13); the remaining 38.46% were diagnosed with malignancies (5/13). Infection was significantly associated to the presence of splenic disease (P = 0.013). There was no difference in the prevalence of infection between dogs with benign and malignant splenic lesions (P = 0.69); however B. canis was more prevalent in dogs with hemangiosarcoma (P = 0.006). VBP infection could be involved in the pathogenesis of splenic disease. The immunological role of the spleen could predispose to alterations of this organ in infected dogs. Interestingly, all dogs with B. canis infection were diagnosed with hemangiosarcoma in the present survey. As previously reported, results support that VBP diagnosis could be improved by analysis of samples from different tissues. The sample size included here warrants further investigation. The online version of this article (doi:10.1186/s13071-017-2074-z) contains supplementary material, which is available to authorized users. 22017-01-0120172017-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/186179https://dx.doi.org/urn:doi:10.1186/s13071-017-2074-zreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1861792026-06-06T12:50:31Z
dc.title.none.fl_str_mv Molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease
title Molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease
spellingShingle Molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease
Movilla, Rebeca
Canine
Vector-borne disease
Spleen
Polymerase chain reaction
Hemangiosarcoma
Babesia canis
title_short Molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease
title_full Molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease
title_fullStr Molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease
title_full_unstemmed Molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease
title_sort Molecular detection of vector-borne pathogens in blood and splenic samples from dogs with splenic disease
dc.creator.none.fl_str_mv Movilla, Rebeca
Altet Sanahujes, Laura
Serrano, Lorena
Tabar, Maria Dolores
Roura, Xavier|||0000-0002-1098-2381
author Movilla, Rebeca
author_facet Movilla, Rebeca
Altet Sanahujes, Laura
Serrano, Lorena
Tabar, Maria Dolores
Roura, Xavier|||0000-0002-1098-2381
author_role author
author2 Altet Sanahujes, Laura
Serrano, Lorena
Tabar, Maria Dolores
Roura, Xavier|||0000-0002-1098-2381
author2_role author
author
author
author
dc.subject.none.fl_str_mv Canine
Vector-borne disease
Spleen
Polymerase chain reaction
Hemangiosarcoma
Babesia canis
topic Canine
Vector-borne disease
Spleen
Polymerase chain reaction
Hemangiosarcoma
Babesia canis
description The spleen is a highly perfused organ involved in the immunological control and elimination of vector-borne pathogens (VBP), which could have a fundamental role in the pathogenesis of splenic disease. This study aimed to evaluate certain VBP in samples from dogs with splenic lesions. Seventy-seven EDTA-blood and 64 splenic tissue samples were collected from 78 dogs with splenic disease in a Mediterranean area. Babesia spp., Bartonella spp., Ehrlichia/Anaplasma spp., Hepatozoon canis, Leishmania infantum, hemotropic Mycoplasma spp. and Rickettsia spp. were targeted using PCR assays. Sixty EDTA-blood samples from dogs without evidence of splenic lesions were included as a control group. More than half (51.56%) of the biopsies (33/64) were consistent with benign lesions and 48.43% (31/64) with malignancy, mostly hemangiosarcoma (25/31). PCR yielded positive results in 13 dogs with spleen alterations (16.67%), for Babesia canis (n = 3), Babesia gibsoni (n = 2), hemotropic Mycoplasma spp. (n = 2), Rickettsia massiliae (n = 1) and "Babesia vulpes" (n = 1), in blood; and for B. canis, B. gibsoni, Ehrlichia canis and L. infantum (n = 1 each), in spleen. Two control dogs (3.3%) were positive for B. gibsoni and H. canis (n = 1 each). Benign lesions were detected in the 61.54% of infected dogs (8/13); the remaining 38.46% were diagnosed with malignancies (5/13). Infection was significantly associated to the presence of splenic disease (P = 0.013). There was no difference in the prevalence of infection between dogs with benign and malignant splenic lesions (P = 0.69); however B. canis was more prevalent in dogs with hemangiosarcoma (P = 0.006). VBP infection could be involved in the pathogenesis of splenic disease. The immunological role of the spleen could predispose to alterations of this organ in infected dogs. Interestingly, all dogs with B. canis infection were diagnosed with hemangiosarcoma in the present survey. As previously reported, results support that VBP diagnosis could be improved by analysis of samples from different tissues. The sample size included here warrants further investigation. The online version of this article (doi:10.1186/s13071-017-2074-z) contains supplementary material, which is available to authorized users.
publishDate 2017
dc.date.none.fl_str_mv 2
2017-01-01
2017
2017-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/186179
https://dx.doi.org/urn:doi:10.1186/s13071-017-2074-z
url https://ddd.uab.cat/record/186179
https://dx.doi.org/urn:doi:10.1186/s13071-017-2074-z
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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