Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum)

Ghambarali, Saeed; Abdollahi, Mohammad Reza; Zolnorian, Haidar; Moosavi, Sayyed Saeed; Seguí-Simarro, Jose M.|||0000-0001-7672-4169

Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum)

[EN] Synthetic seed technology is a convenient alternative to conventional multiplication in potato. In this work, we studied and optimized the process of alginate encapsulation of axillary buds derived from potato minituber sprouts (PMSs). We assayed different concentrations of sodium alginate, CaC...

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Detalhes bibliográficos
Autores:	Ghambarali, Saeed, Abdollahi, Mohammad Reza, Zolnorian, Haidar, Moosavi, Sayyed Saeed, Seguí-Simarro, Jose M.\|\|\|0000-0001-7672-4169
Formato:	artículo
Fecha de publicación:	2016
País:	España
Recursos:	Universitat Politècnica de València (UPV)
Repositorio:	RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:	inglés
OAI Identifier:	oai:riunet.upv.es:10251/97042
Acesso em linha:	https://riunet.upv.es/handle/10251/97042
Access Level:	acceso abierto
Palavra-chave:	Artificial seed Alginate Coco peat 24-Epibrassinolide Germplasm storage Synseed GENETICA

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spelling	Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum)Ghambarali, SaeedAbdollahi, Mohammad RezaZolnorian, HaidarMoosavi, Sayyed SaeedSeguí-Simarro, Jose M.\|\|\|0000-0001-7672-4169Artificial seedAlginateCoco peat24-EpibrassinolideGermplasm storageSynseedGENETICA[EN] Synthetic seed technology is a convenient alternative to conventional multiplication in potato. In this work, we studied and optimized the process of alginate encapsulation of axillary buds derived from potato minituber sprouts (PMSs). We assayed different concentrations of sodium alginate, CaCl2 and matrix culture media, different explant sizes, different concentrations of 24-epibrassinolide (EBr) applied at different stages of the encapsulation process, different planting substrates and different cold storage periods in order to determine the best conditions for encapsulation in cvs. Sante and Agria. The conditions that resulted in the greatest regrowth rates and speed in both cultivars involved the choice of 2 3 mmlong buds, a 2 days pre-culture of buds in culture medium supplemented with 10-6 M EBr, encapsulation in 3 % sodium alginate with 1 % CaCl2 and full-strength MS culture medium, regrowth in solid MS culture medium and then transfer to coco peat (coir fiber pith) for conversion into plantlets. We also found that buds encapsulated under these conditions maintained the initial viability rates for up to 120 days in Sante and 90 days in Agria , although regrowth speed decreased after 60 days in both cultivars. We demonstrate the possibility of producing synthetic seeds efficiently using axillary buds derived from PMSs.Springer-VerlagDepartamento de BiotecnologíaInstituto Universitario de Conservación y Mejora de la Agrodiversidad ValencianaEscuela Técnica Superior de Ingeniería Agronómica y del Medio NaturalRepositorio Institucional de la Universitat Politècnica de València Riunet20162016-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://riunet.upv.es/handle/10251/97042reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Reserva de todos los derechoshttp://rightsstatements.org/vocab/InC/1.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/970422026-06-13T07:49:27Z
dc.title.none.fl_str_mv	Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum)
title	Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum)
spellingShingle	Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum) Ghambarali, Saeed Artificial seed Alginate Coco peat 24-Epibrassinolide Germplasm storage Synseed GENETICA
title_short	Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum)
title_full	Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum)
title_fullStr	Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum)
title_full_unstemmed	Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum)
title_sort	Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum)
dc.creator.none.fl_str_mv	Ghambarali, Saeed Abdollahi, Mohammad Reza Zolnorian, Haidar Moosavi, Sayyed Saeed Seguí-Simarro, Jose M.\|\|\|0000-0001-7672-4169
author	Ghambarali, Saeed
author_facet	Ghambarali, Saeed Abdollahi, Mohammad Reza Zolnorian, Haidar Moosavi, Sayyed Saeed Seguí-Simarro, Jose M.\|\|\|0000-0001-7672-4169
author_role	author
author2	Abdollahi, Mohammad Reza Zolnorian, Haidar Moosavi, Sayyed Saeed Seguí-Simarro, Jose M.\|\|\|0000-0001-7672-4169
author2_role	author author author author
dc.contributor.none.fl_str_mv	Departamento de Biotecnología Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv	Artificial seed Alginate Coco peat 24-Epibrassinolide Germplasm storage Synseed GENETICA
topic	Artificial seed Alginate Coco peat 24-Epibrassinolide Germplasm storage Synseed GENETICA
description	[EN] Synthetic seed technology is a convenient alternative to conventional multiplication in potato. In this work, we studied and optimized the process of alginate encapsulation of axillary buds derived from potato minituber sprouts (PMSs). We assayed different concentrations of sodium alginate, CaCl2 and matrix culture media, different explant sizes, different concentrations of 24-epibrassinolide (EBr) applied at different stages of the encapsulation process, different planting substrates and different cold storage periods in order to determine the best conditions for encapsulation in cvs. Sante and Agria. The conditions that resulted in the greatest regrowth rates and speed in both cultivars involved the choice of 2 3 mmlong buds, a 2 days pre-culture of buds in culture medium supplemented with 10-6 M EBr, encapsulation in 3 % sodium alginate with 1 % CaCl2 and full-strength MS culture medium, regrowth in solid MS culture medium and then transfer to coco peat (coir fiber pith) for conversion into plantlets. We also found that buds encapsulated under these conditions maintained the initial viability rates for up to 120 days in Sante and 90 days in Agria , although regrowth speed decreased after 60 days in both cultivars. We demonstrate the possibility of producing synthetic seeds efficiently using axillary buds derived from PMSs.
publishDate	2016
dc.date.none.fl_str_mv	2016 2016-01-01
dc.type.none.fl_str_mv	journal article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://riunet.upv.es/handle/10251/97042
url	https://riunet.upv.es/handle/10251/97042
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 Reserva de todos los derechos http://rightsstatements.org/vocab/InC/1.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf application/pdf
dc.publisher.none.fl_str_mv	Springer-Verlag
publisher.none.fl_str_mv	Springer-Verlag
dc.source.none.fl_str_mv	reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname:Universitat Politècnica de València (UPV)
instname_str	Universitat Politècnica de València (UPV)
reponame_str	RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection	RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score	15,301629

Optimization of the conditions for production of synthetic seeds by encapsulation of axillary buds derived from minituber sprouts in potato (Solanum tuberosum)

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